AB308184

Anti-Norovirus VP1 antibody [HL1672]

Recombinant
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Rabbit Recombinant Monoclonal CAPSD antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Norovirus samples.

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Supplier Data

Western blot - Anti-Norovirus VP1 antibody [HL1672] (AB308184)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Norovirus VP1 antibody [HL1672] (ab308184) diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

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Western blot - Anti-Norovirus VP1 antibody [HL1672] (ab308184) at 1/5000 dilution

Lane 1:

Non-transfected (–) HEK-293T whole cell lysate at 30 µg

Lanes 2 - 3:

Transfected (+) HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56.6 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1672

Isotype

IgG

Carrier free

Yes

Reacts with

Norovirus

Applications

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Norovirus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Norovirus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Norovirus Capsid protein VP1 also known as the Norwalk Capsid protein is a major structural component of noroviruses. The mass of this protein is approximately 58 kilodaltons. It is expressed within the viral particles forming the outer shell or capsid which plays an important role in protection and infectivity of the virus. VP1 is also the main target for capsid ELISA tests which are employed for virus detection.

Biological function summary

Norovirus Capsid protein VP1 assembles into icosahedral particles forming the structure that encases the viral RNA. It works as part of a complex with other molecular groups to facilitate the virus’s entry into host cells by binding to histo-blood group antigens found on epithelial cells of the digestive tract. This protein’s structure allows it to adapt and evade the host immune response which plays role in the virus's infectivity.

Pathways

Norovirus Capsid protein VP1 involves in pathways related to viral replication and immune evasion. Inside the host it participates in interactions that inhibit immune signaling pathways. These interactions often involve proteins like the Golgi-associated mechanics to promote efficient assembly and release of viral particles. VP1's interaction with cellular machinery ensures ongoing propagation of the virus.

The Norovirus Capsid protein VP1 directly connects to gastroenteritis a leading cause of acute viral gastroenteritis. It links to disease progression by aiding viral invasion and persistence in the gastrointestinal tract. The VP1's interactions with the immune system and histo-blood group antigens contribute to high transmissibility and variability of infection severity among different individuals.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Norovirus Capsid protein VP1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Norovirus VP1 antibody [HL1672]

Western blot - Anti-Norovirus VP1 antibody [HL1672] (AB308184)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Proteins & Peptides

Assay Kits

Primary Antibodies

Product promise