Rabbit Polyclonal NOSIP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NOSIP aa 1-300.
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Publications
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- American journal of translational research 9:2535-25472017Glutamine with probiotics attenuates intestinal inflammation and oxidative stress in a rat burn injury model through altered gene aberrant methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen-Yu Gong et. al.PubMed 28560003
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NOSIP aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y314
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0) |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Target data
Function
E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).
Alternative names
CGI-25, NOSIP, Nitric oxide synthase-interacting protein, E3 ubiquitin-protein ligase NOSIP, RING-type E3 ubiquitin transferase NOSIP, eNOS-interacting protein
Recommended products
Rabbit Polyclonal NOSIP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NOSIP aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NOSIP aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y314
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NOSIP also known as Nitric Oxide Synthase Interacting Protein is a protein involved in the regulation of nitric oxide synthase activity. The protein has a molecular mass of approximately 30 kDa. It expresses mainly in tissues like the brain heart and skeletal muscle. NOSIP's primary role involves interacting with endothelial nitric oxide synthase (eNOS) which is integral to various cellular processes.
- Biological function summary
NOSIP influences nitric oxide production by modulating eNOS activity. It forms a complex with eNOS which affects the localization and activity of this enzyme. This interaction can lead to changes in endothelial function and vascular tone. NOSIP acts as an essential regulator of intracellular nitric oxide signaling impacting processes like vasodilation and blood pressure regulation.
- Pathways
NOSIP plays a role in the nitric oxide signaling pathway and interacts with proteins like eNOS. This pathway is important for regulating vascular homeostasis and immune response. NOSIP influences eNOS phosphorylation states modulating its activity and affecting nitric oxide availability. By mediating eNOS activity NOSIP helps maintain balance in processes related to vascular and immune system functions.
- Associated diseases and disorders
NOSIP is associated with cardiovascular diseases and neurodegenerative disorders. Changes in its function or expression can impact nitric oxide production contributing to hypertension or atherosclerosis. Through its interaction with eNOS NOSIP may indirectly influence neurodegenerative conditions like Alzheimer's disease by affecting vascular health. Understanding these connections offers insights into potential therapeutic targets for managing these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NOSIP antibody (ab102087)
12% SDS PAGE
All lanes: Western blot - Anti-NOSIP antibody (ab102087) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOSIP antibody (ab102087)
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ab102087 at 1/100 dilution.
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