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AB102087

Anti-NOSIP antibody

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(1 Publication)

Rabbit Polyclonal NOSIP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NOSIP aa 1-300.

View Alternative Names

CGI-25, NOSIP, Nitric oxide synthase-interacting protein, E3 ubiquitin-protein ligase NOSIP, RING-type E3 ubiquitin transferase NOSIP, eNOS-interacting protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOSIP antibody (AB102087)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOSIP antibody (AB102087)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labelling NOSIP protein at cytoplasm and nucleus with ab102087 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOSIP antibody (AB102087)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOSIP antibody (AB102087)

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ab102087 at 1/100 dilution.

Western blot - Anti-NOSIP antibody (AB102087)
  • WB

Unknown

Western blot - Anti-NOSIP antibody (AB102087)

12% SDS PAGE

All lanes:

Western blot - Anti-NOSIP antibody (ab102087) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NOSIP aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9Y314

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Complementary Products

Primary Antibodies

AB199956

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RabMAb|Recombinant

Western blot - Anti-eNOS antibody [EPR19296] (AB199956)

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Primary Antibodies

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Anti-Neurofilament heavy polypeptide antibody [EPR20020]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

  • 0
  • 0
Secondary Antibodies

AB150155

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

  • 5
  • 2
Primary Antibodies

AB199116

Anti-CAMTA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMTA1 antibody (AB199116)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOSIP also known as Nitric Oxide Synthase Interacting Protein is a protein involved in the regulation of nitric oxide synthase activity. The protein has a molecular mass of approximately 30 kDa. It expresses mainly in tissues like the brain heart and skeletal muscle. NOSIP's primary role involves interacting with endothelial nitric oxide synthase (eNOS) which is integral to various cellular processes.
Biological function summary

NOSIP influences nitric oxide production by modulating eNOS activity. It forms a complex with eNOS which affects the localization and activity of this enzyme. This interaction can lead to changes in endothelial function and vascular tone. NOSIP acts as an essential regulator of intracellular nitric oxide signaling impacting processes like vasodilation and blood pressure regulation.

Pathways

NOSIP plays a role in the nitric oxide signaling pathway and interacts with proteins like eNOS. This pathway is important for regulating vascular homeostasis and immune response. NOSIP influences eNOS phosphorylation states modulating its activity and affecting nitric oxide availability. By mediating eNOS activity NOSIP helps maintain balance in processes related to vascular and immune system functions.

NOSIP is associated with cardiovascular diseases and neurodegenerative disorders. Changes in its function or expression can impact nitric oxide production contributing to hypertension or atherosclerosis. Through its interaction with eNOS NOSIP may indirectly influence neurodegenerative conditions like Alzheimer's disease by affecting vascular health. Understanding these connections offers insights into potential therapeutic targets for managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed : 11149895, PubMed : 15548660, PubMed : 16135813).
See full target information NOSIP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 9:2535-2547 PubMed28560003

2017

Glutamine with probiotics attenuates intestinal inflammation and oxidative stress in a rat burn injury model through altered gene aberrant methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Yu Gong,Zhi-Qiang Yuan,Zhi-Wei Dong,Yi-Zhi Peng
View all publications
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Product promise

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