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Rabbit Recombinant Monoclonal Notch1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 2 publications.

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Images

Western blot - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (AB221603), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (AB221603), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (AB221603), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (AB221603), expandable thumbnail
  • Western blot - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (AB221603), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Expected
Not recommended
Tested
Expected
Expected
Cow
Predicted
Not recommended
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

7 products for Alternative Version

Target data

Function

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).

Alternative names

TAN1, NOTCH1, Neurogenic locus notch homolog protein 1, Notch 1, hN1, Translocation-associated notch protein TAN-1

Recommended products

Rabbit Recombinant Monoclonal Notch1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP1238Y
Purification technique
Affinity purification Protein A
Specificity

80% identities with Notch 2 and 81% with Notch 3

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab221603 is the carrier-free version of Anti-Notch1 antibody [EP1238Y] ab52627.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Notch1 also known as Notch-1 is a transmembrane receptor involved in cell fate decisions. It belongs to the Notch protein family and has a molecular weight of around 270 kDa. Notch1 is expressed widely particularly in tissues such as blood cells and neuronal tissue. The receptor consists of extracellular EGF-like repeats a transmembrane domain and an intracellular domain that translocates to the nucleus upon activation. Notch1 plays a critical role in intercellular communication through ligand engagement which then triggers a series of proteolytic cleavages that release the Notch intracellular domain (NICD) for nuclear translocation.

Biological function summary

Notch1 is essential in various cellular processes including differentiation proliferation and apoptosis. It functions as part of a complex signaling pathway that regulates these processes. Notch1 interacts with other elements like Jagged and Delta/Serrate/LAG-2 (DSL) family ligands to control gene expression patterns that determine cell lineage outcomes. This interaction affects the development of many systems such as the immune and nervous systems. Consequently Notch1 significantly influences the formation of organs and the maintenance of stem cell populations.

Pathways

Notch1 plays a significant role in both the Notch signaling and the Wnt signaling pathways. In the Notch signaling pathway Notch1 upon ligand binding partners with CSL (CBF1/RBP-Jκ in mammals) and Mastermind-like proteins for transcriptional regulation. This pathway interlinks with the Wnt pathway that involves proteins like β-catenin affecting the regulation of gene transcription. The interplay between Notch1 and these pathways is fundamental in determining outcomes in cell proliferation and differentiation emphasizing the interconnected nature of signaling networks.

Associated diseases and disorders

Notch1 is associated with T-cell acute lymphoblastic leukemia and breast cancer. Altered Notch1 signaling often through gain-of-function mutations can drive oncogenesis by disrupting normal cell differentiation and promoting uncontrolled proliferation. In T-cell acute lymphoblastic leukemia aberrant activation of Notch1 leads to increased expression of target genes working closely with related proteins like c-Myc. In breast cancer dysregulation of Notch1 signaling may facilitate tumor growth and metastasis indicating its role as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603), expandable thumbnail

    Western blot - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Notch1 antibody [EP1238Y] ab52627).

    Lanes 1- 2: Merged signal (red and green). Green - Anti-Notch1 antibody [EP1238Y] ab52627 observed at 110-120 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    Anti-Notch1 antibody [EP1238Y] ab52627 was shown to react with Notch1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human NOTCH1 knockout HeLa cell line ab261762 (knockout cell lysate Human NOTCH1 knockout HeLa cell lysate ab257006) was used. Wild-type HeLa and NOTCH1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-Notch1 antibody [EP1238Y] ab52627 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Notch1 antibody [EP1238Y] (Anti-Notch1 antibody [EP1238Y] ab52627) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human NOTCH1 knockout HeLa cell line (Human NOTCH1 knockout HeLa cell line ab261762)

    Lane 2: NOTCH1 knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 272 kDa

    Observed band size: 110-120 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603)

    Immunohistochemical staining of paraffin-embedded human brain with purified Anti-Notch1 antibody [EP1238Y] ab52627 at a dilution of 1/150. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).

  • Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603)

    Immunofluorescent staining of HeLa cells fixed with 4% PFA using purified Anti-Notch1 antibody [EP1238Y] ab52627 at a dilution of 1/150. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary and the sample was stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 after Anti-Notch1 antibody [EP1238Y] ab52627 as the negative control and is shown in the bottom right hand panel.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).

  • Flow Cytometry (Intracellular) - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603)

    Intracellular Flow Cytometry analysis of permeabilized HeLa cells (2% PFA, pink) with purified Anti-Notch1 antibody [EP1238Y] ab52627 at a 1/200 dilution, or negative control rabbit monoclonal IgG (green). The secondary antibody was FITC goat anti-rabbit.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).

  • Western blot - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603), expandable thumbnail

    Western blot - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Notch1 antibody [EP1238Y] ab52627).

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-Notch1 antibody [EP1238Y] ab52627 observed at 110-120 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    Anti-Notch1 antibody [EP1238Y] ab52627 was shown to react with Notch1 in wild-type HAP1 cells in western blot. Loss of signal was observed when NOTCH1 knockout sample was used. Wild-type and NOTCH1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with Anti-Notch1 antibody [EP1238Y] ab52627 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Notch1 antibody [EP1238Y] (Anti-Notch1 antibody [EP1238Y] ab52627) at 1 µg/mL

    Lane 1: Wild-type HAP1 cell lysate at 40 µg

    Lane 2: NOTCH1 knockout HAP1 cell lysate at 40 µg

    Lane 3: MOLT-4 cell lysate at 20 µg

    Lane 4: HepG2 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 272 kDa

    Observed band size: 110-120 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603)

    Immunohistochemical staining of paraffin-embedded human brain with unpurified Anti-Notch1 antibody [EP1238Y] ab52627 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).

  • Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603)

    Immunofluorescent staining of HeLa cells fixed with 4% PFA using unpurified Anti-Notch1 antibody [EP1238Y] ab52627 at a dilution of 1/100. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary and the sample was stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).

  • Western blot - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603), expandable thumbnail

    Western blot - Anti-Notch1 antibody [EP1238Y] - BSA and Azide free (ab221603)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).
    Western blot: Anti-NOTCH1 antibody [EP1238Y] (Anti-Notch1 antibody [EP1238Y] ab52627) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-Notch1 antibody [EP1238Y] ab52627 was shown to bind specifically to NOTCH1. A band was observed at 110-120 kDa in wild-type HL-60 cell lysates with no signal observed at this size in NOTCH1 knockout cell line. To generate this image, wild-type and NOTCH1 knockout HL-60 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Notch1 antibody [EP1238Y] (Anti-Notch1 antibody [EP1238Y] ab52627) at 1/1000 dilution

    Lane 1: Wild-type HL-60 cell lysate at 20 µg

    Lane 2: NOTCH1 knockout HL-60 cell lysate at 20 µg

    Lane 3: Wild-type HAP1 cell lysate at 20 µg

    Lane 4: NOTCH1 knockout HAP1 cell lysate at 20 µg

    Lane 5: MOLT-4 cell lysate at 20 µg

    Lane 6: HepG2 cell lysate at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 110-120 kDa

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