Skip to main content

Rabbit Recombinant Monoclonal Notch1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (AB246693), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (AB246693), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (AB246693), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (AB246693), expandable thumbnail
  • Western blot - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (AB246693), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Tested
Not recommended
Expected
Expected
Expected
Cow
Predicted
Not recommended
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

5 products for Alternative Version

Target data

Function

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).

Alternative names

TAN1, NOTCH1, Neurogenic locus notch homolog protein 1, Notch 1, hN1, Translocation-associated notch protein TAN-1

Recommended products

Rabbit Recombinant Monoclonal Notch1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP1238Y
Purification technique
Affinity purification Protein A
Concentration
Loading...
Purification notes

Endotoxin level is less than 1 EU/ml as determined by the TAL test.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab246693 is the carrier-free version of Anti-Notch1 antibody [EP1238Y] ab52627.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Notch1 also known as Notch-1 is a transmembrane receptor involved in cell fate decisions. It belongs to the Notch protein family and has a molecular weight of around 270 kDa. Notch1 is expressed widely particularly in tissues such as blood cells and neuronal tissue. The receptor consists of extracellular EGF-like repeats a transmembrane domain and an intracellular domain that translocates to the nucleus upon activation. Notch1 plays a critical role in intercellular communication through ligand engagement which then triggers a series of proteolytic cleavages that release the Notch intracellular domain (NICD) for nuclear translocation.

Biological function summary

Notch1 is essential in various cellular processes including differentiation proliferation and apoptosis. It functions as part of a complex signaling pathway that regulates these processes. Notch1 interacts with other elements like Jagged and Delta/Serrate/LAG-2 (DSL) family ligands to control gene expression patterns that determine cell lineage outcomes. This interaction affects the development of many systems such as the immune and nervous systems. Consequently Notch1 significantly influences the formation of organs and the maintenance of stem cell populations.

Pathways

Notch1 plays a significant role in both the Notch signaling and the Wnt signaling pathways. In the Notch signaling pathway Notch1 upon ligand binding partners with CSL (CBF1/RBP-Jκ in mammals) and Mastermind-like proteins for transcriptional regulation. This pathway interlinks with the Wnt pathway that involves proteins like β-catenin affecting the regulation of gene transcription. The interplay between Notch1 and these pathways is fundamental in determining outcomes in cell proliferation and differentiation emphasizing the interconnected nature of signaling networks.

Associated diseases and disorders

Notch1 is associated with T-cell acute lymphoblastic leukemia and breast cancer. Altered Notch1 signaling often through gain-of-function mutations can drive oncogenesis by disrupting normal cell differentiation and promoting uncontrolled proliferation. In T-cell acute lymphoblastic leukemia aberrant activation of Notch1 leads to increased expression of target genes working closely with related proteins like c-Myc. In breast cancer dysregulation of Notch1 signaling may facilitate tumor growth and metastasis indicating its role as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693)

    Immunohistochemical staining of paraffin-embedded human brain with purified Anti-Notch1 antibody [EP1238Y] ab52627 at a dilution of 1/150.

    A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693)

    Verification of gene expression array data by immunohistochemical analysis of Notch 1 expression in subcutaneous tumors and lung metastases from a human melanoma (MeWo) xenograft experiment in mice.

    Immunohistochemical staining for Notch1 expression (Anti-Notch1 antibody [EP1238Y] ab52627, red) in subcutaneous tumors and lung metastases (both panels) of MeWo (Human malignant melanoma cell line) cells.

    All scale bars: 50 μm.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693)

    Immunofluorescent staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells fixed with 4% PFA using purified Anti-Notch1 antibody [EP1238Y] ab52627 at a dilution of 1/150.

    An Alexa Fluor® 555 goat anti-rabbit was used as the secondary and the sample was stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 after Anti-Notch1 antibody [EP1238Y] ab52627 as the negative control and is shown in the bottom right hand panel.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).

  • Flow Cytometry (Intracellular) - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693)

    Intracellular Flow Cytometry analysis of permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (2% PFA, pink) withpurified Anti-Notch1 antibody [EP1238Y] ab52627 at a 1/200 dilution, or negative control rabbit monoclonal IgG (green). The secondary antibody was FITC goat anti-rabbit.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).

  • Western blot - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693), expandable thumbnail

    Western blot - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Notch1 antibody [EP1238Y] ab52627).

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-Notch1 antibody [EP1238Y] ab52627 observed at 110-120 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    Anti-Notch1 antibody [EP1238Y] ab52627 was shown to react with Notch1 in wild-type HAP1 cells in western blot. Loss of signal was observed when NOTCH1 knockout sample was used. Wild-type and NOTCH1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3pc milk before incubation with Anti-Notch1 antibody [EP1238Y] ab52627 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Notch1 antibody [EP1238Y] (Anti-Notch1 antibody [EP1238Y] ab52627) at 1 µg/mL

    Lane 1: Wild-type HAP1 cell lysate at 40 µg

    Lane 2: NOTCH1 knockout HAP1 cell lysate at 40 µg

    Lane 3: MOLT-4 cell lysate at 20 µg

    Lane 4: HepG2 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 272 kDa

    Observed band size: 110-120 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693)

    Immunohistochemical staining of paraffin-embedded human brain with unpurified Anti-Notch1 antibody [EP1238Y] ab52627 at a dilution of 1/100.

    A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y] - Low endotoxin, Azide free (ab246693)

    Immunofluorescent staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells fixed with 4% PFA using unpurified Anti-Notch1 antibody [EP1238Y] ab52627 at a dilution of 1/100.

    An Alexa Fluor® 555 goat anti-rabbit was used as the secondary and the sample was stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Notch1 antibody [EP1238Y] ab52627).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com