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AB322541

Anti-Notch1 antibody [EPR25682-42] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Notch1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

TAN1, NOTCH1, Neurogenic locus notch homolog protein 1, Notch 1, hN1, Translocation-associated notch protein TAN-1

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Sandwich ELISA - Anti-Notch1 antibody [EPR25682-42] - BSA and Azide free (Detector) (AB322541)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Notch1 antibody [EPR25682-42] - BSA and Azide free (Detector) (AB322541)

Sandwich ELISA of ab322561 with the capture antibody ab322540 dilution at 2 ug/mL and detector antibody ab322541 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-Notch1 antibody [EPR25682-42] - BSA and Azide free (Detector) (AB322541)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Notch1 antibody [EPR25682-42] - BSA and Azide free (Detector) (AB322541)

Interpolated concentration of native Notch1 was measured at different sample concentrations. Undiluted samples are as follows : human plasma (EDTA) 1.25%. Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Notch1 antibody [EPR25682-42] - BSA and Azide free (Detector) (AB322541)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Notch1 antibody [EPR25682-42] - BSA and Azide free (Detector) (AB322541)

Interpolated concentration of native Notch1 was measured at different sample concentrations. Undiluted samples are as follows : Hela cell extract (250 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Notch1 antibody [EPR25682-42] - BSA and Azide free (Detector) (AB322541)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Notch1 antibody [EPR25682-42] - BSA and Azide free (Detector) (AB322541)

Interpolated concentration of native Notch1 was measured at different sample concentrations. Undiluted sample is as follows : MOLT-4 supernatant 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25682-42

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab322541 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Notch1. The recommended pair for sandwich ELISA is:

Capture: ab322540, Human Notch1 Capture Antibody (unconjugated)

Detector: ab322541, Human Notch1 Detector Antibody (unconjugated)

The reference range value is 7.81-1000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
See full target information NOTCH1
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Complementary Products

Assay Kits

AB155437

Human Notch-1 ELISA Kit (TAN-1)

Sandwich ELISA - Human Notch-1 ELISA Kit (TAN-1) (AB155437)

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Product promise

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