Anti-Notch1 intracellular domain antibody

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Notch1 intracellular domain often called NICD plays a significant role in cell signaling. NICD is approximately 120 kDa in mass and is primarily expressed in various tissues especially during development. It also shows expression in multiple cell types including T-cells in the immune system. The Notch1 protein undergoes proteolytic cleavage to release the NICD which then translocates to the nucleus to influence gene expression.

Biological function summary

The Notch1 intracellular domain acts as a transcriptional regulator within the Notch signaling pathway. It forms a complex with other proteins including the transcription factors RBPJ (Recombination Signal Binding Protein for Immunoglobulin Kappa J Region) and MAML (Mastermind-like) to modulate the transcription of target genes. This domain influences cell fate decisions differentiation and proliferation in many developmental processes. The Notch signaling pathway is often described as a communication system between adjacent cells conferring the ability to respond appropriately to developmental and environmental cues.

Pathways

The Notch1 intracellular domain has a critical function in the Notch signaling pathway and is inherently involved in the regulation of the canonical Wnt pathway. The NICD regulates interaction with the signaling protein β-catenin linking it to non-canonical Wnt signaling as well. Notch1 interacts closely with Delta-like and Jagged proteins which act as ligands to initiate the signaling cascade across cell membranes. The interactions among these components ensure precise control over cell differentiation and proliferation.

Associated diseases and disorders

The Notch1 intracellular domain holds significance in the progression of cancers such as T-cell acute lymphoblastic leukemia (T-ALL) where aberrant Notch1 signaling leads to uncontrolled cell proliferation. In addition NICD has a connection to Alagille syndrome a genetic disorder affecting the liver heart and other organs which involves mutations in Jagged1. The links between NICD and these conditions highlight the importance of controlled Notch1 activity in maintaining tissue homeostasis and preventing pathological changes.