AB8387

Anti-Notch1 intracellular domain antibody

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(5 Publications)

Rabbit Polyclonal Notch1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

TAN1, NOTCH1, Neurogenic locus notch homolog protein 1, Notch 1, hN1, Translocation-associated notch protein TAN-1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 intracellular domain antibody (AB8387)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 intracellular domain antibody (AB8387)

ab8387 staining Notch1 in Human brain cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde. Samples were incubated with primary antibody (1/500). Nuclear counter stain using hematoxylin (purple).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Notch1 antibody is directed against human NOTCH 1. No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Notch1 intracellular domain often called NICD plays a significant role in cell signaling. NICD is approximately 120 kDa in mass and is primarily expressed in various tissues especially during development. It also shows expression in multiple cell types including T-cells in the immune system. The Notch1 protein undergoes proteolytic cleavage to release the NICD which then translocates to the nucleus to influence gene expression.

Biological function summary

The Notch1 intracellular domain acts as a transcriptional regulator within the Notch signaling pathway. It forms a complex with other proteins including the transcription factors RBPJ (Recombination Signal Binding Protein for Immunoglobulin Kappa J Region) and MAML (Mastermind-like) to modulate the transcription of target genes. This domain influences cell fate decisions differentiation and proliferation in many developmental processes. The Notch signaling pathway is often described as a communication system between adjacent cells conferring the ability to respond appropriately to developmental and environmental cues.

Pathways

The Notch1 intracellular domain has a critical function in the Notch signaling pathway and is inherently involved in the regulation of the canonical Wnt pathway. The NICD regulates interaction with the signaling protein β-catenin linking it to non-canonical Wnt signaling as well. Notch1 interacts closely with Delta-like and Jagged proteins which act as ligands to initiate the signaling cascade across cell membranes. The interactions among these components ensure precise control over cell differentiation and proliferation.

The Notch1 intracellular domain holds significance in the progression of cancers such as T-cell acute lymphoblastic leukemia (T-ALL) where aberrant Notch1 signaling leads to uncontrolled cell proliferation. In addition NICD has a connection to Alagille syndrome a genetic disorder affecting the liver heart and other organs which involves mutations in Jagged1. The links between NICD and these conditions highlight the importance of controlled Notch1 activity in maintaining tissue homeostasis and preventing pathological changes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).

See full target information NOTCH1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 12:1365705 PubMed38572484

2024

A maternal dorsoventral prepattern revealed by an asymmetric distribution of ventralizing molecules before fertilization in .

Applications

Unspecified application

Species

Unspecified reactive species

Aitana M Castro Colabianchi,Nicolás G González Pérez,Lucía F Franchini,Silvia L López

Cell 176:1379-1392.e14 PubMed30773315

2019

Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating TAZ Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Xia,Daniel Gütl,Vanessa Zheden,Carl-Philipp Heisenberg

Development (Cambridge, England) 145: PubMed29866901

2018

Notch1 is asymmetrically distributed from the beginning of embryogenesis and controls the ventral center.

Applications

Unspecified application

Species

Unspecified reactive species

Aitana M Castro Colabianchi,Diego R Revinski,Paula I Encinas,María Verónica Baez,Renato J Monti,Mateo Rodríguez Abinal,Laurent Kodjabachian,Lucía F Franchini,Silvia L López

Clinical cancer research : an official journal of 17:5686-94 PubMed21771876

2011

Notch1 expression, which is related to p65 Status, is an independent predictor of prognosis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dake Chu,Yi Zhou,Zixi Zhang,Yuming Li,Jipeng Li,Jianyong Zheng,Hongwei Zhang,Qingchuan Zhao,Weizhong Wang,Rui'an Wang,Gang Ji

Cell cycle (Georgetown, Tex.) 6:418-29 PubMed17329972

2007

Notch, Myc and breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Argiris Efstratiadis,Matthias Szabolcs,Apostolos Klinakis

View all publications

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Product promise

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Anti-Notch1 intracellular domain antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch1 intracellular domain antibody (AB8387)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise