AB245325

Anti-Notch2 antibody

Name: Anti-Notch2 antibody (ab245325) | Abcam
Brand: Abcam Limited
SKU: AB245325

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(4 Publications)

Rabbit Polyclonal Notch2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NOTCH2 aa 2400 to C-terminus.

View Alternative Names

Neurogenic locus notch homolog protein 2, Notch 2, hN2, NOTCH2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (AB245325)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (AB245325)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Notch2 using ab245325 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Supplier Data

Immunoprecipitation - Anti-Notch2 antibody (AB245325)

Notch2 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab245325 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab245325 at 1 μg/ml.

Lane 1 : ab245325 IP in HeLa whole cell lysate.

Lane 2 : Control IgG in HeLa wholoe cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Notch2 antibody (ab245325)

Predicted band size: 265 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-Notch2 antibody (AB245325)

All lanes:

Western blot - Anti-Notch2 antibody (ab245325) at 0.4 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 265 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human NOTCH2 aa 2400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q04721

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245325 was affinity purified using an epitope specific to Notch2 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Notch2 also known as neurogenic locus notch homolog protein 2 is a transmembrane receptor protein that plays a critical role in cell differentiation and development. It is part of the Notch signaling pathway which is highly conserved across species. The protein weighs approximately 270 kilodaltons and is primarily found in the kidney liver and various other tissues. Notch2 activation occurs through interactions with specific ligands leading to the release of its intracellular domain which enters the nucleus to regulate gene expression.

Biological function summary

Notch2 modulates important developmental processes including lineage specification and cell fate determination. This protein is vital in maintaining the homeostasis of several organs and tissues and it does this by being part of the Notch signaling complex. Additionally Notch2 contributes to hematopoiesis influencing the balance between different blood cell types. Researchers have also noted its role in maintaining stem cell populations and facilitating the proper function of the immune system.

Pathways

Notch2 integrates into the canonical Notch signaling pathway which impacts cellular communication and gene transcription. This pathway interlinks with the Wnt signaling pathway influencing tissue regeneration and cancer development. Notch2 itself associates with proteins like Jagged1 and Delta-like ligands which are imperative to the signaling cascade. Cross-talk between these pathways highlights its importance in ensuring precise regulation of cellular functions and maintaining tissue integrity.

Notch2 has links to Alagille syndrome and certain malignancies like breast cancer. Mutations or dysregulation in the Notch2 signaling pathway can lead to these conditions. In Alagille syndrome for instance the interplay between Notch2 and Jagged1 mutations alters bile duct development leading to liver and heart problems. Also its role in various cancers suggests that altered Notch2 signaling contributes to uncontrolled cell proliferation and tumor progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed : 21378985, PubMed : 21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed : 29149593). Positively regulates self-renewal of liver cancer cells (PubMed : 25985737).

See full target information NOTCH2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 55:443-456 PubMed36797542

2023

Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Dan Li,Wei Xing,Shao-Chuan Wang,You-Bin Li,Hao Jiang,Han-Xuan Zheng,Xiao-Ming Li,Jing Yang,De-Bin Guo,Xiao-Yu Xie,Ren-Qing Jiang,Chao Fan,Lei Li,Xiang Xu,Jun Fei

Journal of assisted reproduction and genetics 40:201-210 PubMed36471201

2022

CircSTAM inhibits migration and invasion of trophoblast cells by regulating miR-148a-5p/PTEN axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingfeng Chen,Jinyu Yan,Haiyan Zhang,Jia Xu,Xiaopei Chen

PeerJ 10:e13402 PubMed35646490

2022

Down-regulated NEDD4L facilitates tumor progression through activating Notch signaling in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Lin,Xuan Wu,Yuanxue Jiang,Caijiu Deng,Xi Luo,Jianjun Han,Jiazhu Hu,Xiaolong Cao

Bioengineered 12:4032-4043 PubMed34288822

2021

Circular RNA FAT atypical cadherin 1 (circFAT1)/microRNA-525-5p/spindle and kinetochore-associated complex subunit 1 (SKA1) axis regulates oxaliplatin resistance in breast cancer by activating the notch and Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Yao,Xiaoqin Li,Lihua Cheng,Xiuhua Wu,Bobo Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Notch2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (AB245325)

Immunoprecipitation - Anti-Notch2 antibody (AB245325)

Western blot - Anti-Notch2 antibody (AB245325)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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