Rabbit Polyclonal Notch2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NOTCH2 aa 2400 to C-terminus.
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- Bioengineered 12:4032-40432021Circular RNA FAT atypical cadherin 1 (circFAT1)/microRNA-525-5p/spindle and kinetochore-associated complex subunit 1 (SKA1) axis regulates oxaliplatin resistance in breast cancer by activating the notch and Wnt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Yao,Xiaoqin Li,Lihua Cheng,Xiuhua Wu,Bobo WuPubMed 34288822
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NOTCH2 aa 2400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q04721
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed:21378985, PubMed:21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed:29149593). Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
Alternative names
Neurogenic locus notch homolog protein 2, Notch 2, hN2, NOTCH2
Recommended products
Rabbit Polyclonal Notch2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NOTCH2 aa 2400 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NOTCH2 aa 2400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q04721
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab245325 was affinity purified using an epitope specific to Notch2 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Notch2 also known as neurogenic locus notch homolog protein 2 is a transmembrane receptor protein that plays a critical role in cell differentiation and development. It is part of the Notch signaling pathway which is highly conserved across species. The protein weighs approximately 270 kilodaltons and is primarily found in the kidney liver and various other tissues. Notch2 activation occurs through interactions with specific ligands leading to the release of its intracellular domain which enters the nucleus to regulate gene expression.
- Biological function summary
Notch2 modulates important developmental processes including lineage specification and cell fate determination. This protein is vital in maintaining the homeostasis of several organs and tissues and it does this by being part of the Notch signaling complex. Additionally Notch2 contributes to hematopoiesis influencing the balance between different blood cell types. Researchers have also noted its role in maintaining stem cell populations and facilitating the proper function of the immune system.
- Pathways
Notch2 integrates into the canonical Notch signaling pathway which impacts cellular communication and gene transcription. This pathway interlinks with the Wnt signaling pathway influencing tissue regeneration and cancer development. Notch2 itself associates with proteins like Jagged1 and Delta-like ligands which are imperative to the signaling cascade. Cross-talk between these pathways highlights its importance in ensuring precise regulation of cellular functions and maintaining tissue integrity.
- Associated diseases and disorders
Notch2 has links to Alagille syndrome and certain malignancies like breast cancer. Mutations or dysregulation in the Notch2 signaling pathway can lead to these conditions. In Alagille syndrome for instance the interplay between Notch2 and Jagged1 mutations alters bile duct development leading to liver and heart problems. Also its role in various cancers suggests that altered Notch2 signaling contributes to uncontrolled cell proliferation and tumor progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunoprecipitation - Anti-Notch2 antibody (ab245325)
Notch2 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab245325 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab245325 at 1 μg/ml.
Lane 1: ab245325 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa wholoe cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-Notch2 antibody (ab245325)
Developed using the ECL technique.
Predicted band size: 265 kDa
Exposure time: 30s
Western blot - Anti-Notch2 antibody (ab245325)
All lanes: Western blot - Anti-Notch2 antibody (ab245325) at 0.4 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Predicted band size: 265 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab245325)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Notch2 using ab245325 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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