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Rabbit Polyclonal Notch2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 65 publications. Immunogen corresponding to Synthetic Peptide within Human NOTCH2 aa 1700-1750.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (AB8926), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (AB8926), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (AB8926), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human NOTCH2 aa 1700-1750. The exact immunogen used to generate this antibody is proprietary information. Database link Q04721

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Expected
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

1/400 - 1/2000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed:21378985, PubMed:21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed:29149593). Positively regulates self-renewal of liver cancer cells (PubMed:25985737).

Alternative names

Recommended products

Rabbit Polyclonal Notch2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 65 publications. Immunogen corresponding to Synthetic Peptide within Human NOTCH2 aa 1700-1750.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human NOTCH2 aa 1700-1750. The exact immunogen used to generate this antibody is proprietary information. Database link Q04721
Purity

Whole antiserum

Specificity

The Notch2 protein has many isoforms. The immunogenic sequence for the anti-Notch2 antibody is identical to the same region in full length (260+ kDa) as well as the cleaved forms ~82 kDa each (Notch 2 extracellular truncation & Notch 2 intracellular domain) and the post translationally modified forms thereof.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926), expandable thumbnail
    Image from Murta, Daniel et al., PLoS ONE 8.8 (2013): e72767. Fig 2J. doi: 10.1371/journal.pone.0072767 P. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is expressed in germ cells entering meiosis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926), expandable thumbnail
    Image from Murta, Daniel et al., PLoS ONE 8.8 (2013): e72767. Fig 2J. doi: 10.1371/journal.pone.0072767 P.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is ubiquitously expressed in germ cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Notch2 with ab8926 at 1/500 dilution. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

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Product protocols

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