Rabbit Polyclonal Notch2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 65 publications. Immunogen corresponding to Synthetic Peptide within Human NOTCH2 aa 1700-1750.
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- Environmental toxicology 38:1628-16402023Di-(2-ethylhexyl) phthalate (DEHP) promoted hepatic lipid accumulation by activating Notch signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuezhu Zhang,Honghao Qian,Jia Wang,Ying Zhu,Xiaohan Miao,Xu Li,Jianli Yin,Ruxuan Zhang,Jiaming Ye,Chuanyi Huo,Weisen Zhao,Lin YePubMed 36988346
- International journal of molecular sciences 23:2022Differences in Immunohistochemical and Ultrastructural Features between Podocytes and Parietal Epithelial Cells (PECs) Are Observed in Developing, Healthy Postnatal, and Pathologically Changed Human Kidneys.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarin Ogorevc,Ivona Kosovic,Natalija Filipovic,Ivana Bocina,Marija Juric,Benjamin Benzon,Snjezana Mardesic,Katarina Vukojevic,Marijan Saraga,Boris Kablar,Mirna Saraga-BabicPubMed 35886848
- Methods in molecular biology (Clifton, N.J.) 2472:173-1852022Immunolocalization of Notch Signaling in Mouse Preimplantation Embryos.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisabete Silva,Patrícia Diniz,Alexandre Trindade,Mariana Batista,Ana Torres,António Duarte,Luís Lopes-da-CostaPubMed 35674900
- Nature communications 13:15372022GIT1 protects against breast cancer growth through negative regulation of Notch.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSongbai Zhang,Ayako Miyakawa,Malin Wickström,Cecilia Dyberg,Lauri Louhivuori,Manuel Varas-Godoy,Kati Kemppainen,Shigeaki Kanatani,Dagmara Kaczynska,Ivar Dehnisch Ellström,Lotta Elfman,Pauliina Kronqvist,Heli Repo,Katsuhiko Mikoshiba,Cecilia Sahlgren,John Inge Johnsen,Per UhlénPubMed 35318302
- Translational lung cancer research 10:4161-41732022alteration in -mutated lung adenocarcinoma leads to histological small-cell carcinoma transformation under EGFR-TKI treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHayato Koba,Hideharu Kimura,Taro Yoneda,Naohiko Ogawa,Kota Tanimura,Yuichi Tambo,Takashi Sone,Kazuyoshi Hosomichi,Atsushi Tajima,Kazuo KasaharaPubMed 35004247
- Cell death and differentiation 29:366-3802021Gm364 coordinates MIB2/DLL3/Notch2 to regulate female fertility through AKT activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang-Jian Chen,Na-Na Zhang,Chun-Xiang Zhou,Zhi-Xia Yang,Yan-Ru Li,Teng Zhang,Cong-Rong Li,Xin Wang,Yang Wang,Zi-Bin Wang,Zheng-Rong Xia,Zhen-Bo Wang,Cui-Lian Zhang,Yi-Chun Guan,Qing-Yuan Sun,Dong ZhangPubMed 34635817
- Arteriosclerosis, thrombosis, and vascular biology 41:e427-e4392021Fibrous Caps in Atherosclerosis Form by Notch-Dependent Mechanisms Common to Arterial Media Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos J Martos-Rodríguez,Julián Albarrán-Juárez,Daniel Morales-Cano,Ainoa Caballero,Donal MacGrogan,José Luis de la Pompa,Laura Carramolino,Jacob F BentzonPubMed 34261328
- Respiratory research 22:442021LncRNA SNHG16 promotes pulmonary fibrosis by targeting miR-455-3p to regulate the Notch2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPanpan Liu,Lei Zhao,Yuxia Gu,Meilan Zhang,Hongchang Gao,Yingxia MengPubMed 33549106
- Aging 13:2941-29582021Up-regulation of miR-204 inhibits proliferation, invasion and apoptosis of gallbladder cancer cells by targeting Notch2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaohua Zhang et. al.PubMed 33460397
- Cell cycle (Georgetown, Tex.) 19:3622-36312020DCAF13 promotes triple-negative breast cancer metastasis by mediating DTX3 mRNA degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiazhe Liu et. al.PubMed 33300431
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 98.6% Whole serum, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NOTCH2 aa 1700-1750. The exact immunogen used to generate this antibody is proprietary information. Database link Q04721
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed:21378985, PubMed:21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed:29149593). Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
Alternative names
Neurogenic locus notch homolog protein 2, Notch 2, hN2, NOTCH2
Recommended products
Rabbit Polyclonal Notch2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 65 publications. Immunogen corresponding to Synthetic Peptide within Human NOTCH2 aa 1700-1750.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 98.6% Whole serum, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NOTCH2 aa 1700-1750. The exact immunogen used to generate this antibody is proprietary information. Database link Q04721
- Purity
- Whole antiserum
- Specificity
The Notch2 protein has many isoforms. The immunogenic sequence for the anti-Notch2 antibody is identical to the same region in full length (260+ kDa) as well as the cleaved forms ~82 kDa each (Notch 2 extracellular truncation & Notch 2 intracellular domain) and the post translationally modified forms thereof.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Notch2 also known as neurogenic locus notch homolog protein 2 is a transmembrane receptor protein that plays a critical role in cell differentiation and development. It is part of the Notch signaling pathway which is highly conserved across species. The protein weighs approximately 270 kilodaltons and is primarily found in the kidney liver and various other tissues. Notch2 activation occurs through interactions with specific ligands leading to the release of its intracellular domain which enters the nucleus to regulate gene expression.
- Biological function summary
Notch2 modulates important developmental processes including lineage specification and cell fate determination. This protein is vital in maintaining the homeostasis of several organs and tissues and it does this by being part of the Notch signaling complex. Additionally Notch2 contributes to hematopoiesis influencing the balance between different blood cell types. Researchers have also noted its role in maintaining stem cell populations and facilitating the proper function of the immune system.
- Pathways
Notch2 integrates into the canonical Notch signaling pathway which impacts cellular communication and gene transcription. This pathway interlinks with the Wnt signaling pathway influencing tissue regeneration and cancer development. Notch2 itself associates with proteins like Jagged1 and Delta-like ligands which are imperative to the signaling cascade. Cross-talk between these pathways highlights its importance in ensuring precise regulation of cellular functions and maintaining tissue integrity.
- Associated diseases and disorders
Notch2 has links to Alagille syndrome and certain malignancies like breast cancer. Mutations or dysregulation in the Notch2 signaling pathway can lead to these conditions. In Alagille syndrome for instance the interplay between Notch2 and Jagged1 mutations alters bile duct development leading to liver and heart problems. Also its role in various cancers suggests that altered Notch2 signaling contributes to uncontrolled cell proliferation and tumor progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Image from Murta, Daniel et al., PLoS ONE 8.8 (2013): e72767. Fig 2J. doi: 10.1371/journal.pone.0072767 P. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is expressed in germ cells entering meiosis.
Image from Murta, Daniel et al., PLoS ONE 8.8 (2013): e72767. Fig 2J. doi: 10.1371/journal.pone.0072767 P.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is ubiquitously expressed in germ cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Notch2 with ab8926 at 1/500 dilution. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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