Rabbit Polyclonal Notch2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 65 publications. Immunogen corresponding to Synthetic Peptide within Human NOTCH2 aa 1700-1750.
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- Environmental toxicology 38:1628-16402023Di-(2-ethylhexyl) phthalate (DEHP) promoted hepatic lipid accumulation by activating Notch signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuezhu Zhang,Honghao Qian,Jia Wang,Ying Zhu,Xiaohan Miao,Xu Li,Jianli Yin,Ruxuan Zhang,Jiaming Ye,Chuanyi Huo,Weisen Zhao,Lin YePubMed 36988346
- International journal of molecular sciences 23:2022Differences in Immunohistochemical and Ultrastructural Features between Podocytes and Parietal Epithelial Cells (PECs) Are Observed in Developing, Healthy Postnatal, and Pathologically Changed Human Kidneys.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarin Ogorevc,Ivona Kosovic,Natalija Filipovic,Ivana Bocina,Marija Juric,Benjamin Benzon,Snjezana Mardesic,Katarina Vukojevic,Marijan Saraga,Boris Kablar,Mirna Saraga-BabicPubMed 35886848
- Methods in molecular biology (Clifton, N.J.) 2472:173-1852022Immunolocalization of Notch Signaling in Mouse Preimplantation Embryos.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisabete Silva,Patrícia Diniz,Alexandre Trindade,Mariana Batista,Ana Torres,António Duarte,Luís Lopes-da-CostaPubMed 35674900
- Nature communications 13:15372022GIT1 protects against breast cancer growth through negative regulation of Notch.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSongbai Zhang,Ayako Miyakawa,Malin Wickström,Cecilia Dyberg,Lauri Louhivuori,Manuel Varas-Godoy,Kati Kemppainen,Shigeaki Kanatani,Dagmara Kaczynska,Ivar Dehnisch Ellström,Lotta Elfman,Pauliina Kronqvist,Heli Repo,Katsuhiko Mikoshiba,Cecilia Sahlgren,John Inge Johnsen,Per UhlénPubMed 35318302
- Translational lung cancer research 10:4161-41732022alteration in -mutated lung adenocarcinoma leads to histological small-cell carcinoma transformation under EGFR-TKI treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHayato Koba,Hideharu Kimura,Taro Yoneda,Naohiko Ogawa,Kota Tanimura,Yuichi Tambo,Takashi Sone,Kazuyoshi Hosomichi,Atsushi Tajima,Kazuo KasaharaPubMed 35004247
- Cell death and differentiation 29:366-3802021Gm364 coordinates MIB2/DLL3/Notch2 to regulate female fertility through AKT activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang-Jian Chen,Na-Na Zhang,Chun-Xiang Zhou,Zhi-Xia Yang,Yan-Ru Li,Teng Zhang,Cong-Rong Li,Xin Wang,Yang Wang,Zi-Bin Wang,Zheng-Rong Xia,Zhen-Bo Wang,Cui-Lian Zhang,Yi-Chun Guan,Qing-Yuan Sun,Dong ZhangPubMed 34635817
- Arteriosclerosis, thrombosis, and vascular biology 41:e427-e4392021Fibrous Caps in Atherosclerosis Form by Notch-Dependent Mechanisms Common to Arterial Media Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos J Martos-Rodríguez,Julián Albarrán-Juárez,Daniel Morales-Cano,Ainoa Caballero,Donal MacGrogan,José Luis de la Pompa,Laura Carramolino,Jacob F BentzonPubMed 34261328
- Respiratory research 22:442021LncRNA SNHG16 promotes pulmonary fibrosis by targeting miR-455-3p to regulate the Notch2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPanpan Liu,Lei Zhao,Yuxia Gu,Meilan Zhang,Hongchang Gao,Yingxia MengPubMed 33549106
- Aging 13:2941-29582021Up-regulation of miR-204 inhibits proliferation, invasion and apoptosis of gallbladder cancer cells by targeting Notch2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaohua Zhang et. al.PubMed 33460397
- Cell cycle (Georgetown, Tex.) 19:3622-36312020DCAF13 promotes triple-negative breast cancer metastasis by mediating DTX3 mRNA degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiazhe Liu et. al.PubMed 33300431
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human NOTCH2 aa 1700-1750. The exact immunogen used to generate this antibody is proprietary information. Database link Q04721
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed:21378985, PubMed:21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed:29149593). Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
Alternative names
Neurogenic locus notch homolog protein 2, Notch 2, hN2, NOTCH2
Recommended products
Rabbit Polyclonal Notch2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 65 publications. Immunogen corresponding to Synthetic Peptide within Human NOTCH2 aa 1700-1750.
Alternative names
Neurogenic locus notch homolog protein 2, Notch 2, hN2, NOTCH2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human NOTCH2 aa 1700-1750. The exact immunogen used to generate this antibody is proprietary information. Database link Q04721
- Purity
Whole antiserum
- Specificity
The Notch2 protein has many isoforms. The immunogenic sequence for the anti-Notch2 antibody is identical to the same region in full length (260+ kDa) as well as the cleaved forms ~82 kDa each (Notch 2 extracellular truncation & Notch 2 intracellular domain) and the post translationally modified forms thereof.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Image from Murta, Daniel et al., PLoS ONE 8.8 (2013): e72767. Fig 2J. doi: 10.1371/journal.pone.0072767 P. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is expressed in germ cells entering meiosis.
Image from Murta, Daniel et al., PLoS ONE 8.8 (2013): e72767. Fig 2J. doi: 10.1371/journal.pone.0072767 P.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is ubiquitously expressed in germ cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Notch2 with ab8926 at 1/500 dilution. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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