AB245699

Anti-Notch2 antibody [B9]

Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Notch2 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Notch2.

View Alternative Names

Neurogenic locus notch homolog protein 2, Notch 2, hN2, NOTCH2

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Notch2 antibody [B9] (AB245699)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Notch2 antibody [B9] (AB245699)

PFA fixed, 0.15% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Notch2 using ab245699 at 10 μg/ml, 1 hr (green) followed by Alexa Fluor^® 488 secondary antibody (1 μg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Mouse Notch2. The exact immunogen used to generate this antibody is proprietary information.

O35516

Specificity

An engineered ScFv fragment antibody that binds to both mouse mNRR2 and human hNRR2 but not NRR1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Notch2 also known as neurogenic locus notch homolog protein 2 is a transmembrane receptor protein that plays a critical role in cell differentiation and development. It is part of the Notch signaling pathway which is highly conserved across species. The protein weighs approximately 270 kilodaltons and is primarily found in the kidney liver and various other tissues. Notch2 activation occurs through interactions with specific ligands leading to the release of its intracellular domain which enters the nucleus to regulate gene expression.

Biological function summary

Notch2 modulates important developmental processes including lineage specification and cell fate determination. This protein is vital in maintaining the homeostasis of several organs and tissues and it does this by being part of the Notch signaling complex. Additionally Notch2 contributes to hematopoiesis influencing the balance between different blood cell types. Researchers have also noted its role in maintaining stem cell populations and facilitating the proper function of the immune system.

Pathways

Notch2 integrates into the canonical Notch signaling pathway which impacts cellular communication and gene transcription. This pathway interlinks with the Wnt signaling pathway influencing tissue regeneration and cancer development. Notch2 itself associates with proteins like Jagged1 and Delta-like ligands which are imperative to the signaling cascade. Cross-talk between these pathways highlights its importance in ensuring precise regulation of cellular functions and maintaining tissue integrity.

Notch2 has links to Alagille syndrome and certain malignancies like breast cancer. Mutations or dysregulation in the Notch2 signaling pathway can lead to these conditions. In Alagille syndrome for instance the interplay between Notch2 and Jagged1 mutations alters bile duct development leading to liver and heart problems. Also its role in various cancers suggests that altered Notch2 signaling contributes to uncontrolled cell proliferation and tumor progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed : 21378985, PubMed : 21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed : 29149593). Positively regulates self-renewal of liver cancer cells (PubMed : 25985737).

See full target information NOTCH2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 20:491-502 PubMed38819062

2024

Overexpression of low-density lipoprotein receptor prevents neurotoxic polarization of astrocytes via inhibiting NLRP3 inflammasome activation in experimental ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Feng,Juanji Li,Tingting Liu,Shiqi Huang,Xiangliang Chen,Shen Liu,Junshan Zhou,Hongdong Zhao,Ye Hong

Oncology research 30:77-87 PubMed37305324

2023

IGF2BP3-induced activation of EIF5B contributes to progression of hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyin Li,Qian Wang,Hongfeng Liang,Shisheng Chen,Haiwen Chen,Yaoyong Lu,Changfu Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Notch2 antibody [B9]

Immunocytochemistry/ Immunofluorescence - Anti-Notch2 antibody [B9] (AB245699)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise