Rabbit Recombinant Monoclonal Notch2 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.
Images
![Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (AB307701), expandable thumbnail](https://content.abcam.com/products/images/notch2-antibody-epr26111-69-bsa-and-azide-free-ab307701--western-blot-img407422.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (AB307701)")
![Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (AB307701), expandable thumbnail](https://content.abcam.com/products/images/notch2-antibody-epr26111-69-bsa-and-azide-free-ab307701--western-blot-img407421.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (AB307701)")
![Dot Blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (AB307701), expandable thumbnail](https://content.abcam.com/products/images/notch2-antibody-epr26111-69-bsa-and-azide-free-ab307701--dot-blot-img407112.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (AB307701)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Expected | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
Target data
Function
Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed:21378985, PubMed:21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed:29149593). Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
Alternative names
Neurogenic locus notch homolog protein 2, Notch 2, hN2, NOTCH2
Recommended products
Rabbit Recombinant Monoclonal Notch2 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26111-69
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Notch2 also known as neurogenic locus notch homolog protein 2 is a transmembrane receptor protein that plays a critical role in cell differentiation and development. It is part of the Notch signaling pathway which is highly conserved across species. The protein weighs approximately 270 kilodaltons and is primarily found in the kidney liver and various other tissues. Notch2 activation occurs through interactions with specific ligands leading to the release of its intracellular domain which enters the nucleus to regulate gene expression.
- Biological function summary
Notch2 modulates important developmental processes including lineage specification and cell fate determination. This protein is vital in maintaining the homeostasis of several organs and tissues and it does this by being part of the Notch signaling complex. Additionally Notch2 contributes to hematopoiesis influencing the balance between different blood cell types. Researchers have also noted its role in maintaining stem cell populations and facilitating the proper function of the immune system.
- Pathways
Notch2 integrates into the canonical Notch signaling pathway which impacts cellular communication and gene transcription. This pathway interlinks with the Wnt signaling pathway influencing tissue regeneration and cancer development. Notch2 itself associates with proteins like Jagged1 and Delta-like ligands which are imperative to the signaling cascade. Cross-talk between these pathways highlights its importance in ensuring precise regulation of cellular functions and maintaining tissue integrity.
- Associated diseases and disorders
Notch2 has links to Alagille syndrome and certain malignancies like breast cancer. Mutations or dysregulation in the Notch2 signaling pathway can lead to these conditions. In Alagille syndrome for instance the interplay between Notch2 and Jagged1 mutations alters bile duct development leading to liver and heart problems. Also its role in various cancers suggests that altered Notch2 signaling contributes to uncontrolled cell proliferation and tumor progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (ab307701), expandable thumbnail](https://content.abcam.com/products/images/notch2-antibody-epr26111-69-bsa-and-azide-free-ab307701--western-blot-img407422.jpg "Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (ab307701)")
Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (ab307701)
This data was developed using Anti-Notch2 antibody [EPR26111-69] ab307700, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: IMR-32.
Lysates were freshly made and used immediately to minimize protein degradation. The full-length (FL) Notch2 protein and the transmembrane/intracellular region (NTM) are indicated. In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) loading control staining at 1/10000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-Notch2 antibody [EPR26111-69] (Anti-Notch2 antibody [EPR26111-69] ab307700) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 120 kDa, 310 kDa
Exposure time: 180s
![Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (ab307701), expandable thumbnail](https://content.abcam.com/products/images/notch2-antibody-epr26111-69-bsa-and-azide-free-ab307701--western-blot-img407421.jpg "Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (ab307701)")
Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (ab307701)
This data was developed using Anti-Notch2 antibody [EPR26111-69] ab307700, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: K562 (PMID: 23359069).
Lysates were freshly made and used immediately to minimize protein degradation. The full-length (FL) Notch2 protein and the transmembrane/intracellular region (NTM) are indicated. In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) loading control staining at 1/10000 dilution.
Exposure time:
Lanes 1-2: 56 seconds
Lanes 3-4: 180 seconds.All lanes: Western blot - Anti-Notch2 antibody [EPR26111-69] (Anti-Notch2 antibody [EPR26111-69] ab307700) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 3: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 4: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 120 kDa, 310 kDa
![Dot Blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (ab307701), expandable thumbnail](https://content.abcam.com/products/images/notch2-antibody-epr26111-69-bsa-and-azide-free-ab307701--dot-blot-img407112.jpg "Dot Blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (ab307701)")
Dot Blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (ab307701)
This data was developed using Anti-Notch2 antibody [EPR26111-69] ab307700, the same antibody clone in a different buffer formulation.
Dot blot analysis of Notch2 using Anti-Notch2 antibody [EPR26111-69] ab307700 at 1:1000 (0.526 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: Human NOTCH1 recombinant protein fragment
Lane 2: Human NOTCH2 recombinant protein fragment
Lane 3: Human NOTCH3 recombinant protein fragment
Exposure time: 180 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Downloads
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