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AB277799

Anti-NOTCH3 antibody [4HCLC]

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(1 Publication)

Rabbit Recombinant Multiclonal NOTCH3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NOTC3_HUMAN aa 2300 to C-terminus.

View Alternative Names

Neurogenic locus notch homolog protein 3, Notch 3, NOTCH3

1 Images
Western blot - Anti-NOTCH3 antibody [4HCLC] (AB277799)
  • WB

Supplier Data

Western blot - Anti-NOTCH3 antibody [4HCLC] (AB277799)

All lanes:

Western blot - Anti-NOTCH3 antibody [4HCLC] (ab277799) at 2 µg/mL

Lane 1:

MCF7 whole cell extracts at 30 µg

Lane 2:

U-87 MG whole cell extracts at 30 µg

Lane 3:

SH-SY5Y whole cell extracts at 30 µg

Lane 4:

NTERA-2 whole cell extracts at 30 µg

Lane 5:

MDA-MB-23 whole cell extracts at 30 µg

Lane 6:

T-47D whole cell extracts at 30 µg

Lane 7:

U-2 OS whole cell extracts at 30 µg

Lane 8:

A-431 whole cell extracts at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate at 1/2500 dilution

Predicted band size: 244 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

4HCLC

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human NOTC3_HUMAN aa 1800-1850. Database link Q9UM47

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human NOTC3_HUMAN aa 1600-1650. Database link Q9UM47

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Reactivity data

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NOTCH3 protein a significant player in the NOTCH signaling pathway is a transmembrane receptor involved in cell differentiation. Sometimes referred to as 'Notch3' this protein has a molecular weight of approximately 244 kDa. NOTCH3 is widely expressed in vascular smooth muscle cells and tissues such as arteries and lungs. The protein also plays an important role in the communication between adjacent cells because of its location on the cell surface.
Biological function summary

NOTCH3 influences various cellular processes including proliferation apoptosis and differentiation. NOTCH3 does not function alone it is part of a regulatory complex that mediates cellular context and fate decisions. The receptor is cleaved upon ligand binding to facilitate intracellular signaling. This signaling is critical in the development and maintenance of vascular integrity which highlights the protein's importance in both physiological and pathological contexts.

Pathways

NOTCH3 plays a role in the NOTCH signaling pathway essential for cell fate determination and the smooth muscle cell differentiation pathway. Ligands such as Jagged and Delta-like proteins trigger NOTCH3 activation. Upon activation the NOTCH3 intracellular domain translocates to the nucleus where it interacts with transcriptional regulators like RBPJ. This interaction modulates the expression of target genes influencing cell differentiation and proliferation.

NOTCH3 is implicated in Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) a genetic vascular disorder. Mutations in the NOTCH3 gene result in the accumulation of the NOTCH3 protein within arterial walls leading to the characteristic symptoms of CADASIL. Additionally the protein has been linked to pulmonary arterial hypertension through shared pathways with proteins such as BMP2 and SMADs indicating its broader impact on vascular pathophysiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination (PubMed : 15350543). Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).
See full target information NOTC3_HUMAN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 45:266-271 PubMed40151113

2025

Effects of Huluan decotion on cyclophosphamide-induced autoimmune premature ovarian failure in murine models.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Guiling,Zhou Xiaolin,Shen Chengwan,L I Panxiao,Abulizi Abudula
View all publications
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