JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB255839

Anti-NOTCH3 antibody [8G5] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rat Recombinant Monoclonal NOTCH3 antibody. Carrier free. Suitable for ELISA, WB and reacts with Human samples.

View Alternative Names

Neurogenic locus notch homolog protein 3, Notch 3, NOTCH3

3 Images
ELISA - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (AB255839)
  • ELISA

Supplier Data

ELISA - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (AB255839)

This data was produced using the same clone in a different formulation, ab252845.

ab252845 used at 0-4000 ng/ml.

Antigen (Human NOTCH3) used at 1000 ng/ml.

Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

Western blot - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (AB255839)
  • WB

Supplier Data

Western blot - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (AB255839)

This data was produced using the same clone in a different formulation, ab252845.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 30100605 and 24143218).
Negative control : MDA-MB-231.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 70 seconds; Lanes 2-3 : 3 minutes.

All lanes:

Western blot - Anti-NOTCH3 antibody [8G5] (<a href='/en-us/products/primary-antibodies/notch3-antibody-8g5-ab252845'>ab252845</a>) at 1/1000 dilution

Lane 1:

T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 10 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 244 kDa

false

Western blot - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (AB255839)
  • WB

Supplier Data

Western blot - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (AB255839)

This data was produced using the same clone in a different formulation, ab252845.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 30100605 and 24143218).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-NOTCH3 antibody [8G5] (<a href='/en-us/products/primary-antibodies/notch3-antibody-8g5-ab252845'>ab252845</a>) at 1/1000 dilution

All lanes:

U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 244 kDa

false

Exposure time: 3min

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

8G5

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

WB, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab255839 is the carrier-free version of ab252845.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NOTCH3 protein a significant player in the NOTCH signaling pathway is a transmembrane receptor involved in cell differentiation. Sometimes referred to as 'Notch3' this protein has a molecular weight of approximately 244 kDa. NOTCH3 is widely expressed in vascular smooth muscle cells and tissues such as arteries and lungs. The protein also plays an important role in the communication between adjacent cells because of its location on the cell surface.
Biological function summary

NOTCH3 influences various cellular processes including proliferation apoptosis and differentiation. NOTCH3 does not function alone it is part of a regulatory complex that mediates cellular context and fate decisions. The receptor is cleaved upon ligand binding to facilitate intracellular signaling. This signaling is critical in the development and maintenance of vascular integrity which highlights the protein's importance in both physiological and pathological contexts.

Pathways

NOTCH3 plays a role in the NOTCH signaling pathway essential for cell fate determination and the smooth muscle cell differentiation pathway. Ligands such as Jagged and Delta-like proteins trigger NOTCH3 activation. Upon activation the NOTCH3 intracellular domain translocates to the nucleus where it interacts with transcriptional regulators like RBPJ. This interaction modulates the expression of target genes influencing cell differentiation and proliferation.

NOTCH3 is implicated in Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) a genetic vascular disorder. Mutations in the NOTCH3 gene result in the accumulation of the NOTCH3 protein within arterial walls leading to the characteristic symptoms of CADASIL. Additionally the protein has been linked to pulmonary arterial hypertension through shared pathways with proteins such as BMP2 and SMADs indicating its broader impact on vascular pathophysiology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination (PubMed : 15350543). Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).
See full target information NOTC3_HUMAN
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB52627

Anti-Notch1 antibody [EP1238Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Notch1 antibody [EP1238Y] (AB52627)

  • 5
  • 18
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB307701

Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (AB307701)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com