Anti-NOTCH4 antibody [EPNCIR101B]
- RabMAb
- Recombinant
- What is this?
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(4 Publications)
Rabbit Recombinant Monoclonal NOTCH4 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 4 publications.
View Alternative Names
Int-3, Int3, Neurogenic locus notch homolog protein 4, Notch 4
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-NOTCH4 antibody [EPNCIR101B] (AB166605)
Intracellular flow cytometrical analysis of permeabilized Jurkat cells with ab166605 antibody at a dilution of 1/100 (red) compared to a rabbit IgG negative (green).
- WB
Unknown
Western blot - Anti-NOTCH4 antibody [EPNCIR101B] (AB166605)
All lanes:
Western blot - Anti-NOTCH4 antibody [EPNCIR101B] (ab166605) at 1/100 dilution
All lanes:
Mouse lung lysate at 10 µg
Secondary
All lanes:
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 210 kDa
false
- WB
Lab
Western blot - Anti-NOTCH4 antibody [EPNCIR101B] (AB166605)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.
All lanes:
Western blot - Anti-NOTCH4 antibody [EPNCIR101B] (ab166605) at 1/1000 dilution
Lane 1:
Mouse lung tissue lysate at 20 µg
Lane 2:
Mouse heart tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 60 kDa,210 kDa
false
Exposure time: 180s
Related conjugates and formulations (1)
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Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free
Reactivity data
Product details
This antibody was developed as part of a collaboration between the National Cancer Institutes Center for Cancer Research and the lab of Robert Callahan. View antibodies from NCI Center for Cancer Research Collaboration.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Properties and storage information
Form
Purity
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NOTCH4 plays a fundamental role in cell differentiation proliferation and apoptosis. It is part of the NOTCH signaling pathway which involves other receptors like NOTCH1 NOTCH2 and NOTCH3. These receptor-ligand interactions help control the lineage and development of cells during embryogenesis and maintain homeostasis in adult tissues. The NOTCH pathway is important for vascular development and maintaining the vascular system's integrity.
Pathways
NOTCH4 is integral to the NOTCH signaling pathway and the angiogenesis pathway. In the NOTCH signaling activated NOTCH4 interacts with proteins like RBPJ to initiate specific transcriptional programs. It also plays a critical role in the angiogenesis pathway by modulating interactions necessary for endothelial cell behavior and blood vessel formation. These pathways allow NOTCH4 to coordinate with other family members like NOTCH2 to ensure proper cellular outcomes.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (4)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 14:3183 PubMed37268635
2023
Applications
Unspecified application
Species
Unspecified reactive species
Neoplasia (New York, N.Y.) 28:100785 PubMed35390742
2022
Applications
Unspecified application
Species
Unspecified reactive species
American journal of cancer research 7:260-274 PubMed28337375
2017
Applications
Unspecified application
Species
Unspecified reactive species
EBioMedicine 2:1767-84 PubMed26870802
2015
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com