AB249346

Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal NOTCH4 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples.

View Alternative Names

Int-3, Int3, Neurogenic locus notch homolog protein 4, Notch 4

3 Images

Flow Cytometry (Intracellular) - Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (AB249346)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (AB249346)

This data was developed using ab166605, the same antibody clone in a different buffer formulation.

Intracellular flow cytometrical analysis of permeabilized Jurkat cells with ab166605 antibody at a dilution of 1/100 (red) compared to a rabbit IgG negative (green).

Unknown

Western blot - Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (AB249346)

This data was developed using ab166605, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NOTCH4 antibody [EPNCIR101B] (<a href='/en-us/products/primary-antibodies/notch4-antibody-epncir101b-ab166605'>ab166605</a>) at 1/100 dilution

All lanes:

Mouse lung lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 210 kDa

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Lab

Western blot - Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (AB249346)

This data was developed using ab166605, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

All lanes:

Western blot - Anti-NOTCH4 antibody [EPNCIR101B] (<a href='/en-us/products/primary-antibodies/notch4-antibody-epncir101b-ab166605'>ab166605</a>) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,210 kDa

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Exposure time: 180s

Unconjugated

Anti-NOTCH4 antibody [EPNCIR101B]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR101B

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Data from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "Data from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Data from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249346 is the carrier-free version of ab166605.

This antibody was developed as part of a collaboration between the National Cancer Institutes Center for Cancer Research and the lab of Robert Callahan. View antibodies from NCI Center for Cancer Research Collaboration.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOTCH4 also known as Neurogenic locus notch homolog protein 4 is a member of the NOTCH family of receptors. It has a molecular mass of approximately 301 kDa. This protein is expressed in various tissues such as the heart lungs and brain reflecting its involvement in numerous physiological processes. NOTCH4 is a type I transmembrane protein that participates in signal transduction by interacting with adjacent cells through its extracellular domain. It undergoes proteolytic cleavages that release its intracellular domain which then translocates to the nucleus to regulate gene transcription.

Biological function summary

NOTCH4 plays a fundamental role in cell differentiation proliferation and apoptosis. It is part of the NOTCH signaling pathway which involves other receptors like NOTCH1 NOTCH2 and NOTCH3. These receptor-ligand interactions help control the lineage and development of cells during embryogenesis and maintain homeostasis in adult tissues. The NOTCH pathway is important for vascular development and maintaining the vascular system's integrity.

Pathways

NOTCH4 is integral to the NOTCH signaling pathway and the angiogenesis pathway. In the NOTCH signaling activated NOTCH4 interacts with proteins like RBPJ to initiate specific transcriptional programs. It also plays a critical role in the angiogenesis pathway by modulating interactions necessary for endothelial cell behavior and blood vessel formation. These pathways allow NOTCH4 to coordinate with other family members like NOTCH2 to ensure proper cellular outcomes.

Aberrant NOTCH4 signaling connects to lung adenocarcinoma and ischemic stroke. In lung adenocarcinoma NOTCH4 dysregulation may interact with pathways involving proteins like KRAS leading to tumorigenesis. In ischemic stroke altered NOTCH4 function impacts angiogenesis potentially disturbing the blood flow and contributing to stroke pathology alongside other signaling molecules such as VEGF. Proper function and regulation of NOTCH4 appear critical for preventing these disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May regulate branching morphogenesis in the developing vascular system.

See full target information Notch4

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (AB249346)

Western blot - Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (AB249346)

Western blot - Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (AB249346)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise