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AB210568

Anti-Noto antibody

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Rabbit Polyclonal NOTO antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human NOTO aa 1-250.

View Alternative Names

Homeobox protein notochord, NOTO

1 Images
IHC - Wholemount - Anti-Noto antibody (AB210568)
  • IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Noto antibody (AB210568)

Wholemount immunohistochemical analysis of 24-hour post-fertilization zebrafish embryo, labeling Noto with ab210568 at 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Zebrafish

Applications

IHC - Wmt

applications

Immunogen

Recombinant Fragment Protein within Human NOTO aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

A8MTQ0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Noto also known as Not or Notochord is a small nuclear protein with a mass of approximately 43 kDa. It serves a critical role in the notochord development acting as a transcription factor. Scientists often find it expressed in the primitive node and notochordal plate during embryogenesis. Its expression is necessary for the formation of body axis structures influencing the patterning of mesodermal tissues.
Biological function summary

The protein is involved in early vertebrate development impacting the proper development of the notochord which is essential for axial skeleton formation. Noto functions as part of transcriptional regulatory complexes that drive the expression of specific target genes. It interacts with other developmental regulators to ensure the differentiation and organization of midline structures.

Pathways

Noto plays a role in the Wnt and Hedgehog signaling pathways which are important for embryonic development particularly in establishing the body plan. It interacts with proteins like SHH (Sonic Hedgehog) and BMP (Bone Morphogenetic Protein) during these processes. These interactions contribute to the precise orchestration of signals required for tissue specification and the establishment of bilateral symmetry.

Dysregulation of Noto expression associates with congenital spine defects such as scoliosis and spina bifida. Abnormalities in Noto function also link with developmental disorders like caudal dysgenesis. Disruptions in its interaction with SHH can result in improper neural tube closure highlighting its significance in human developmental diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription regulator acting downstream of both FOXA2 and Brachyury (T) during notochord development. Required for node morphogenesis. Is essential for cilia formation in the posterior notochord (PNC) and for left-right patterning; acts upstream of FOXJ1 and RFX3 in this process and is required for the expression of various components important for axonemal assembly and function. Plays a role in regulating axial versus paraxial cell fate. Activates the transcription of ciliary proteins C11orf97 homolog, FAM183B and SPACA9 in the embryonic ventral node (By similarity).
See full target information NOTO
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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