AB106448

Anti-NOTUM antibody

Name: Anti-NOTUM antibody (ab106448) | Abcam
Brand: Abcam Limited
SKU: AB106448
Price: 545 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal NOTUM antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human NOTUM.

View Alternative Names

OK/SW-CL.30, NOTUM, Palmitoleoyl-protein carboxylesterase NOTUM, hNOTUM

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOTUM antibody (AB106448)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOTUM antibody (AB106448)

ab106448 at 2.5μg/ml staining NOTUM in Human brain by Immunohistochemistry.

Unknown

Western blot - Anti-NOTUM antibody (AB106448)

Lane 1:

Western blot - Anti-NOTUM antibody (ab106448) at 0.5 µg/mL

Lane 2:

Western blot - Anti-NOTUM antibody (ab106448) at 1 µg/mL

All lanes:

Human kidney tissue lysate at 15 µg

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human NOTUM. The exact immunogen used to generate this antibody is proprietary information.

Q6P988

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOTUM is a lipase-like enzyme that functions mechanically to remove lipid modifications specifically palmitoleate from the Wnt proteins. It is alternatively known as palmitoleoyl-protein carboxylesterase. The molecular mass of NOTUM is approximately 53 kDa. NOTUM is expressed mainly in tissues such as the liver kidneys and gut where it plays a role in regulating signaling pathways.

Biological function summary

NOTUM inhibits the Wnt signaling pathway by cleaving the lipid moiety attached to Wnt proteins making it inactive. NOTUM acts independently and is not part of larger protein complexes. This regulation of Wnt signaling is important for maintaining cellular processes such as cell proliferation differentiation and migration especially during development and tissue homeostasis.

Pathways

NOTUM primarily influences the Wnt signaling pathway. This pathway is significant for embryonic development and adult homeostasis. By inactivating Wnt proteins NOTUM indirectly affects other pathway components like β-catenin which is an important mediator in the Wnt pathway. NOTUM ensures the balance of Wnt signaling preventing overactivation that could lead to abnormal cell growth.

NOTUM's role in regulating Wnt signaling links it to conditions such as colorectal cancer and osteoporosis. In cancers overexpression of NOTUM can contribute to Wnt pathway dysregulation while its inhibition may present a therapeutic angle. In osteoporosis alterations in NOTUM activity can affect bone formation processes through its influence on Wnt-related proteins like LRP5/6. Understanding NOTUM's interactions in these diseases may provide insights into novel treatment strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed : 25731175).

See full target information NOTUM

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 15:4174-4192 PubMed40893669

2025

High-efficient discovering the potent anti-Notum agents from herbal medicines for combating glucocorticoid-induced osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqing Song,Feng Zhang,Jia Guo,Yufan Fan,Hairong Zeng,Mengru Sun,Jun Qian,Shenglan Qi,Zihan Chen,Xudong Jin,Yunqing Song,Tian Tian,Zhi Qian,Yao Sun,Zhenhao Tian,Baoqing Yu,Guangbo Ge

Investigative ophthalmology & visual science 65:23 PubMed38345554

2024

5-Aza-2'-Deoxycytidine Ameliorates Choroidal Neovascularization by Inhibiting the Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyuan Wu,Xi Yang,Xiaochan Dai,Xiuping Chen,Minqian Shen,Jinhui Dai,Fei Yuan,Liyang Wang,Yuanzhi Yuan,Yifan Feng

Annals of human genetics 87:18-27 PubMed36465015

2022

The expression of NOTUM in replantation of severed fingers may be an important treatment factor.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Libing Xiao,Danhong Ye,Siyi Zhong,Qiaoyu Yan

Bioengineered 12:5241-5252 PubMed34402722

2021

Notum palmitoleoyl-protein carboxylesterase regulates Fas cell surface death receptor-mediated apoptosis via the Wnt signaling pathway in colon adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Gong,Qiang Niu,Yi Zhou,Yun-Xia Wang,Xuan-Fu Xu,Ke-Zhu Hou

Cancer genomics & proteomics 15:485-497 PubMed30343282

2018

NOTUM Is Involved in the Progression of Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jong Hyuk Yoon,Dayea Kim,Jaeyoon Kim,Hyeongjoo Lee,Jaewang Ghim,Byung Jun Kang,Parkyong Song,Pann-Ghill Suh,Sung Ho Ryu,Taehoon G Lee

Cell metabolism 27:1138-1155.e6 PubMed29719227

2018

A Strategy for Discovery of Endocrine Interactions with Application to Whole-Body Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Marcus M Seldin,Simon Koplev,Prashant Rajbhandari,Laurent Vergnes,Gregory M Rosenberg,Yonghong Meng,Calvin Pan,Thuy M N Phuong,Raffi Gharakhanian,Nam Che,Selina Mäkinen,Diana M Shih,Mete Civelek,Brian W Parks,Eric D Kim,Frode Norheim,Karthickeyan Chella Krishnan,Yehudit Hasin-Brumshtein,Margarete Mehrabian,Markku Laakso,Christian A Drevon,Heikki A Koistinen,Peter Tontonoz,Karen Reue,Rita M Cantor,Johan L M Björkegren,Aldons J Lusis

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOTUM antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOTUM antibody (AB106448)

Western blot - Anti-NOTUM antibody (AB106448)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise