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AB55839

Anti-NOTUM antibody

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(1 Publication)

Rabbit Polyclonal NOTUM antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Notum.

View Alternative Names

Palmitoleoyl-protein carboxylesterase NOTUM

1 Images
Western blot - Anti-NOTUM antibody (AB55839)
  • WB

Unknown

Western blot - Anti-NOTUM antibody (AB55839)

All lanes:

Western blot - Anti-NOTUM antibody (ab55839) at 10 µg/mL

All lanes:

HepG2 cell lysate at 11 µg

Secondary

All lanes:

anti-Rabbit conjugated to HRP at 1/100000 dilution

Predicted band size: 55 kDa

Observed band size: 50 kDa,98 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Notum.

Q8R116

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5-10 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

Source: Rabbits were immunized with recombinant full-length notum protein from mouse.
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.08% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOTUM is a lipase-like enzyme that functions mechanically to remove lipid modifications specifically palmitoleate from the Wnt proteins. It is alternatively known as palmitoleoyl-protein carboxylesterase. The molecular mass of NOTUM is approximately 53 kDa. NOTUM is expressed mainly in tissues such as the liver kidneys and gut where it plays a role in regulating signaling pathways.
Biological function summary

NOTUM inhibits the Wnt signaling pathway by cleaving the lipid moiety attached to Wnt proteins making it inactive. NOTUM acts independently and is not part of larger protein complexes. This regulation of Wnt signaling is important for maintaining cellular processes such as cell proliferation differentiation and migration especially during development and tissue homeostasis.

Pathways

NOTUM primarily influences the Wnt signaling pathway. This pathway is significant for embryonic development and adult homeostasis. By inactivating Wnt proteins NOTUM indirectly affects other pathway components like β-catenin which is an important mediator in the Wnt pathway. NOTUM ensures the balance of Wnt signaling preventing overactivation that could lead to abnormal cell growth.

NOTUM's role in regulating Wnt signaling links it to conditions such as colorectal cancer and osteoporosis. In cancers overexpression of NOTUM can contribute to Wnt pathway dysregulation while its inhibition may present a therapeutic angle. In osteoporosis alterations in NOTUM activity can affect bone formation processes through its influence on Wnt-related proteins like LRP5/6. Understanding NOTUM's interactions in these diseases may provide insights into novel treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
See full target information Notum
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 5:30 PubMed25905045

2015

Comparative proteomic analyses demonstrate enhanced interferon and STAT-1 activation in reovirus T3D-infected HeLa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peyman Ezzati,Krysten Komher,Giulia Severini,Kevin M Coombs
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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