Anti-NOX1 antibody

• WB

Supplier Data

Western blot - Anti-NOX1 antibody (AB121009)

Lysates in RIPA buffer. Detected by chemiluminescence.

All lanes:

Western blot - Anti-NOX1 antibody (ab121009) at 0.5 µg/mL

Lane 1:

CaCo-2 cell lysate at 35 µg

Lane 2:

CaCo-2 + peptide cell lysate at 35 µg

Lane 3:

HeLa cell lysate at 35 µg

Lane 4:

HeLa + peptide cell lysate at 35 µg

Lane 5:

A549 cell lysate at 35 µg

Lane 6:

A549 + peptide cell lysate at 35 µg

Lane 7:

U937 cell lysate at 35 µg

Lane 8:

U937 + peptide cell lysate at 35 µg

false

• WB

CiteAb

Western blot - Anti-NOX1 antibody (AB121009)

NOX1 western blot using anti-NOX1 antibody ab121009. Publication image and figure legend from De Bessa, T. C., Pagano, A., et al., 2019, Cell Death Dis, PubMed 30760703.

ab121009 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab121009 please see the product overview.

Nox NADPH oxidase expression and superoxide generation by HCT116 cells with overactivated KRas.a Nox1, NoxA1, p67phox, NoxO1, and p47phox basal protein expression by western analysis normalized for GADPH. Nox1 immunoblot densities were quantified using Odyssey software. *P < 0.05, ANOVA plus Tukey's multiple comparisons test (n = 3). b RhoGDIα, RhoA, and Rac1 basal protein expression by western analysis normalized for GAPDH. Rac1 immunoblot densities were quantified using Odyssey software. **P < 0.01; ***P < 0.005, ANOVA plus Tukey's multiple comparisons test (n = 3). c Nox1 complex scheme. d Rac1 basal activity, using Rac1 G-LISA activation assay (Cytoskeleton,inc). Test t *p < 0.05, (n = 2). e Rac1 protein expression by western analysis after 72 h of PDIA1 silencing. Rac1 Immunoblot densities were quantified using Odyssey software **P < 0.01; ns : not significant, Student's t-test (n = 3). f Western analysis of NoxO1 and RhoGDIα protein expression before and after PDIA1 silencing, normalized for GAPDH. RhoGDIα immunoblot densities were quantified using Odyssey software, ****P < 0.001; ns : not significant, Student's t-test (n = 3). g Superoxide production after 72h of PDIA1 silencing in HCT116 cells treated or not with 10 μM of NoxA1ds Nox1's peptide inhibitor. EOH 2-hidroxyethidiium relative superoxide production, si-PDI, si-RNA against PDIA1 protein; Scrmb NoxA1ds, NoxA1ds negative control peptide; NoxA1ds, Nox1 peptide inhibitor. *P < 0.05; **P < 0.01, ANOVA plus Tukey's multiple comparisons test (n = 3)

false