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AB106940

Anti-NOX2/gp91phox antibody

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(2 Publications)

Goat Polyclonal NOX2/gp91phox antibody. Suitable for IHC and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CYBB aa 150-200.

View Alternative Names

NOX2, CYBB, Cytochrome b-245 heavy chain, CGD91-phox, Cytochrome b(558) subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, Cytochrome b558 subunit beta

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC

applications

Immunogen

Synthetic Peptide within Human CYBB aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P04839

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHC-species-checked": "testedAndGuaranteed", "IHC-species-dilution-info": "2-4 µg/mL", "IHC-species-notes": "<p></p>" }, "Dog": { "IHC-species-checked": "predicted", "IHC-species-dilution-info": "", "IHC-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOX2 also known as gp91phox or cybb is a membrane-bound protein that plays an important role in the production of reactive oxygen species (ROS). This protein functions as a catalytic component of the NADPH oxidase complex which is primarily found in phagocytes like neutrophils and macrophages. The NOX2 protein has a molecular weight of approximately 91 kDa. It is expressed prominently in the cell membranes of these immune cells where it participates in the body's defense mechanisms.
Biological function summary

NOX2 participates in the generation of superoxide by using NADPH as a substrate an essential function performed by the multi-subunit NADPH oxidase complex. This process helps in the formation of microbicidal ROS which neutrophils and macrophages require for killing pathogens. The gp91phox component NOX2's alternate name partners with p22phox and other cytosolic subunits like p47phox and p67phox to form the fully active enzyme complex. This emphasizes NOX2's role in the immune system's oxidative burst an important antimicrobial response.

Pathways

NOX2 is an integral part of the oxidative burst pathway important in host defense. Its activity connects with the MAPK signaling pathway which can be activated by the presence of ROS produced by NOX2. This pathway involves proteins such as ERK JNK and p38 MAPKs which are related through the signaling events that lead to diverse cellular responses including inflammation and stress responses.

NOX2 dysregulation is associated with chronic granulomatous disease (CGD) a condition characterized by a compromised ability of phagocytes to produce ROS leading to recurrent infections. Additionally NOX2 activity links with cardiovascular diseases where its overproduction of ROS can cause oxidative stress contributing to atherosclerosis. The functioning of p22phox another protein in the NADPH oxidase complex becomes particularly relevant in these contexts as it partners with NOX2 to generate the harmful superoxide in such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
See full target information CYBB
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0296586 PubMed40811673

2025

Cannabidiol improves learning and memory deficits and alleviates anxiety in 12-month-old SAMP8 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Monica N Goodland,Subhashis Banerjee,Michael L Niehoff,Benjamin J Young,Heather Macarthur,Andrew A Butler,John E Morley,Susan A Farr

Clinical and experimental immunology 181:480-90 PubMed25693732

2015

Interleukin-1 family cytokines and their regulatory proteins in normal pregnancy and pre-eclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

J H Southcombe,C W G Redman,I L Sargent,I Granne
View all publications
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Alternative Products

Primary Antibodies

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Complementary Products

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Product promise

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