AB310338

Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal NOX2/gp91phox antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IHC-Fr, ICC/IF, IP and reacts with Mouse, Rat, Transfected cell line, Human samples.

View Alternative Names

NOX2, CYBB, NADPH oxidase 2, CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b-245 heavy chain, Heme-binding membrane glycoprotein gp91phox, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, Cytochrome b558 subunit beta

19 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling NOX2/gp91phox with ab310337 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human spleen.The section was incubated with ab310337 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling NOX2/gp91phox with ab310337 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a CYBB expression vector containing a his tag. No staining on (B) HEK-293T transfected with empty vector containing a his tag. The section was incubated with ab310337 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NOX2/gp91phox with ab310337 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on kupffer cells in human liver.The section was incubated with ab310337 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling NOX2/gp91phox with ab310337 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human skeletal muscle.The section was incubated with ab310337 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 100% methanol-fixed, 0.1% Tween-20 permeabilized NR8383 (rat alveolar macrophage) cells labelling NOX2/gp91phox with ab310337 at 1/50 (10.32 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in NR8383 cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling NOX2/gp91phox with ab310337 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on kupffer cells in rat liver.The section was incubated with ab310337 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 100% methanol-fixed, 0.1% Tween-20 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling NOX2/gp91phox with ab310337 at 1/50 (10.32 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in RAW 264.7 cell line, and no staining in Neuro-2a cell line.Negative control : Neuro-2a (PMID : 23516464).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Immunohistochemistry (Frozen sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling NOX2/gp91phox with ab310337 at 1/50 (10.32 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab310337 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling NOX2/gp91phox with ab310337 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on kupffer cells in mouse liver.The section was incubated with ab310337 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling NOX2/gp91phox with ab310337 at 1/50 (10.32 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab310337 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

Flow Cytometry (Intracellular) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NR8383 (rat alveolar macrophage) cells labelling NOX2/gp91phox with ab310337 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast, Left) / RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling NOX2/gp91phox with ab310337 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Low expression : Neuro-2a (PMID : 23516464).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle(fresh) tissue labeling NOX2/gp91phox with ab310337 at 1/50 (10.32 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on mouse skeletal muscle (PMID : 11376945). The nuclear counterstain was DAPI (Blue). The section was incubated with ab310337 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle(fresh) tissue labeling NOX2/gp91phox with ab310337 at 1/50 (10.32 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on rat skeletal muscle (PMID : 11376945). The nuclear counterstain was DAPI (Blue). The section was incubated with ab310337 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling NOX2/gp91phox with ab310337 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse skeletal muscle.The section was incubated with ab310337 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling NOX2/gp91phox with ab310337 at 1/1000 (0.516 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on rat skeletal muscle.The section was incubated with ab310337 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. NOX2/gp91phox was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab310337 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab310337 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate Lane 2 : ab310337 IP in RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab310337 in RAW 264.7 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 seconds

All lanes:

Immunoprecipitation - Anti-NOX2/gp91phox antibody [EPR28415-13] (<a href='/en-us/products/primary-antibodies/nox2-gp91phox-antibody-epr28415-13-ab310337'>ab310337</a>) at 1/30 dilution

All lanes:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3s

Supplier Data

Western blot - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : skeletal muscle (PMID : 11376945). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200, 000 dilution. Exposure time : 59 seconds

All lanes:

Western blot - Anti-NOX2/gp91phox antibody [EPR28415-13] (<a href='/en-us/products/primary-antibodies/nox2-gp91phox-antibody-epr28415-13-ab310337'>ab310337</a>) at 1/1000 dilution

Lane 1:

Rat spleen tissue lysate at 20 µg

Lane 2:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 65 kDa

false

Exposure time: 59s

Supplier Data

Western blot - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

This data was developed using ab310337, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : Neuro-2a (PMID : 23516464), skeletal muscle (PMID : 11376945). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200, 000 dilution. Exposure time : Lanes 1-2 : 15 seconds; Lanes 3-4 : 59 seconds; Lane 5 : 15 seconds.

All lanes:

Western blot - Anti-NOX2/gp91phox antibody [EPR28415-13] (<a href='/en-us/products/primary-antibodies/nox2-gp91phox-antibody-epr28415-13-ab310337'>ab310337</a>) at 1/1000 dilution

Lane 1:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 5:

NR8383 (rat alveolar macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 65 kDa

false

660 APC

APC Anti-NOX2/gp91phox antibody [EPR28415-13]
578 PE

PE Anti-NOX2/gp91phox antibody [EPR28415-13]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-NOX2/gp91phox antibody [EPR28415-13]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-NOX2/gp91phox antibody [EPR28415-13]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-NOX2/gp91phox antibody [EPR28415-13]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-NOX2/gp91phox antibody [EPR28415-13]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-NOX2/gp91phox antibody [EPR28415-13]
Unconjugated

Anti-NOX2/gp91phox antibody [EPR28415-13]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28415-13

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, Flow Cyt (Intra), IHC-Fr, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The human recommendation is based on the IHC-P results. We do not guarantee WB for human.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell line": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOX2 also known as gp91phox or cybb is a membrane-bound protein that plays an important role in the production of reactive oxygen species (ROS). This protein functions as a catalytic component of the NADPH oxidase complex which is primarily found in phagocytes like neutrophils and macrophages. The NOX2 protein has a molecular weight of approximately 91 kDa. It is expressed prominently in the cell membranes of these immune cells where it participates in the body's defense mechanisms.

Biological function summary

NOX2 participates in the generation of superoxide by using NADPH as a substrate an essential function performed by the multi-subunit NADPH oxidase complex. This process helps in the formation of microbicidal ROS which neutrophils and macrophages require for killing pathogens. The gp91phox component NOX2's alternate name partners with p22phox and other cytosolic subunits like p47phox and p67phox to form the fully active enzyme complex. This emphasizes NOX2's role in the immune system's oxidative burst an important antimicrobial response.

Pathways

NOX2 is an integral part of the oxidative burst pathway important in host defense. Its activity connects with the MAPK signaling pathway which can be activated by the presence of ROS produced by NOX2. This pathway involves proteins such as ERK JNK and p38 MAPKs which are related through the signaling events that lead to diverse cellular responses including inflammation and stress responses.

NOX2 dysregulation is associated with chronic granulomatous disease (CGD) a condition characterized by a compromised ability of phagocytes to produce ROS leading to recurrent infections. Additionally NOX2 activity links with cardiovascular diseases where its overproduction of ROS can cause oxidative stress contributing to atherosclerosis. The functioning of p22phox another protein in the NADPH oxidase complex becomes particularly relevant in these contexts as it partners with NOX2 to generate the harmful superoxide in such diseases.

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Target data

Catalytic subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed : 15338276, PubMed : 36241643, PubMed : 36413210, PubMed : 38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (Probable) (PubMed : 38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed : 19028840, PubMed : 38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (By similarity). NADPH oxidase complex assembly is impaired through interaction with NRROS (By similarity).

See full target information CYBB

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Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunocytochemistry/ Immunofluorescence - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunocytochemistry/ Immunofluorescence - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunohistochemistry (Frozen sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunohistochemistry (Frozen sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Flow Cytometry (Intracellular) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Flow Cytometry (Intracellular) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunohistochemistry (Frozen sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunohistochemistry (Frozen sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Immunoprecipitation - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Western blot - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

Western blot - Anti-NOX2/gp91phox antibody [EPR28415-13] - BSA and Azide free (AB310338)

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