AB198213

Anti-NOX5 antibody

Name: Anti-NOX5 antibody (ab198213) | Abcam
Brand: Abcam Limited
SKU: AB198213
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal NOX5 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NADPH oxidase 5.

View Alternative Names

NADPH oxidase 5, NOX5

1 Images

Supplier Data

Western blot - Anti-NOX5 antibody (AB198213)

Gel : 6% SDS-PAGE

All lanes:

Western blot - Anti-NOX5 antibody (ab198213) at 1/200 dilution

Lane 1:

293T cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

Human testis tissue cell lysate

Lane 4:

Skov3 cell lysate

Lane 5:

Raji cell lysate

Lane 6:

Jurkat cell lysate

Predicted band size: 86 kDa

true

Exposure time: 20s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human NADPH oxidase 5. The exact immunogen used to generate this antibody is proprietary information.

Q96PH1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOX5 also known as NADPH oxidase 5 is a member of the NADPH oxidase family which has a molecular mass of approximately 82 kDa. NOX5 is expressed widely across various tissues including the testes spleen and the fetal kidney. It is distinct because it can generate reactive oxygen species (ROS) in response to changes in intracellular calcium levels operating independently from the activation of other related NADPH oxidase enzymes.

Biological function summary

NOX5 plays a role in producing reactive oxygen species which are important for cellular signaling. The protein sometimes functions as an independent enzyme and does not typically form a complex with other proteins. Its calcium-binding domain is essential for its activity allowing it to respond dynamically to cellular changes. This ability to modulate ROS production implicates NOX5 in processes like cell proliferation migration and endothelial function.

Pathways

NOX5 contributes to cellular oxidative stress response and angiogenesis. The enzyme's activity in ROS generation links it with the MAPK signaling pathway which is important for cell differentiation and senescence. Additionally it's associated with the calcium signaling pathway interacting with proteins such as calmodulin which modulate its activity. These interactions ensure NOX5's role in balancing cellular oxidative signaling and responding to calcium fluctuations.

NOX5 is linked with hypertension and cancer. The enzyme's involvement in ROS production can lead to oxidative stress contributing to vascular dysfunction and the pathogenesis of hypertension. In cancer NOX5-related ROS generation can promote tumor growth and metastasis. It shares connections with other oxidative stress-related proteins like NOX1 and NOX2 which also have critical roles in disease progression through similar mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed : 12686516). Also synthesizes NAADP from its reduced NAADPH form which promotes Ca(2+) signaling during T cell activation (PubMed : 34784249). May play a role in cell growth and apoptosis (PubMed : 12686516).. Isoform v2. Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed : 11483596, PubMed : 14982937, PubMed : 17275676, PubMed : 17587483, PubMed : 21642394, PubMed : 22387196, PubMed : 22427510, PubMed : 24505490, PubMed : 36653838). Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contributes to endothelial response to thrombin (PubMed : 17275676). Regulates redox-dependent processes in lymphocytes and spermatozoa (PubMed : 11483596).. Isoform v1. Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor.. Isoform v5. This isoform lacks calcium-binding domains and was showed to present a NADPH oxidase activity in a calcium-independent manner (PubMed : 17275676, PubMed : 36653838). May be involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin (PubMed : 17275676). However another study showed an absence of oxidase activity (PubMed : 22427510). Subject to rapid degradation (PubMed : 36653838).. Isoform v3. Lacks calcium-dependent NADPH oxidase activity.. Isoform v4. Lacks calcium-dependent NADPH oxidase activity.

See full target information NADPH oxidase 5

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 10:829868 PubMed35174145

2022

Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Accelerate Diabetic Wound Healing Ameliorating Oxidative Stress and Promoting Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenchen Yan,Yan Xv,Ze Lin,Yori Endo,Hang Xue,Yiqiang Hu,Liangcong Hu,Lang Chen,Faqi Cao,Wu Zhou,Peng Zhang,Guohui Liu

Frontiers in pharmacology 11:617270 PubMed33584299

2021

USP7 Inhibition Alleviates HO-Induced Injury in Chondrocytes via Inhibiting NOX4/NLRP3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Liu,Qingbai Liu,Bin Yan,Ziqiang Zhu,Yaozeng Xu

Journal of experimental & clinical cancer research 39:144 PubMed32727517

2020

IFI6 depletion inhibits esophageal squamous cell carcinoma progression through reactive oxygen species accumulation via mitochondrial dysfunction and endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenchuan Liu,Shaorui Gu,Tiancheng Lu,Kaiqing Wu,Lei Li,Chenglai Dong,Yongxin Zhou

Aging 12:8968-8986 PubMed32439831

2020

Inhibition of circulating exosomal microRNA-15a-3p accelerates diabetic wound repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Xiong,Lang Chen,Tao Yu,Chenchen Yan,Wu Zhou,Faqi Cao,Xiaomeng You,Yingqi Zhang,Yun Sun,Jing Liu,Hang Xue,Yiqiang Hu,Dong Chen,Bobin Mi,Guohui Liu

Redox biology 34:101399 PubMed31838004

2019

Redox regulation of nitrosyl-hemoglobin in human erythrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Flavia Dei Zotti,Roxane Verdoy,Davide Brusa,Irina I Lobysheva,Jean-Luc Balligand

Cell death & disease 9:948 PubMed30237423

2018

NADPH oxidase 4 regulates anoikis resistance of gastric cancer cells through the generation of reactive oxygen species and the induction of EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Shangce Du,Ji Miao,Zhouting Zhu,En Xu,Linsen Shi,Shichao Ai,Feng Wang,Xing Kang,Hong Chen,Xiaofeng Lu,Wenxian Guan,Xuefeng Xia

View all publications

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Product promise

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Anti-NOX5 antibody

Western blot - Anti-NOX5 antibody (AB198213)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise