AB191010

Anti-NOX5 antibody - C-terminal

Name: Anti-NOX5 antibody - C-terminal (ab191010) | Abcam
Brand: Abcam Limited
SKU: AB191010

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(15 Publications)

Rabbit Polyclonal NOX5 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human NADPH oxidase 5 aa 700 to C-terminus.

View Alternative Names

NADPH oxidase 5, NOX5

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-NOX5 antibody - C-terminal (AB191010)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOX5 antibody - C-terminal (AB191010)

Immunocytochemistry analysis of HeLa cells, labeling NOX5 using ab191010 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX5 antibody - C-terminal (AB191010)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX5 antibody - C-terminal (AB191010)

Immunohistochemical analysis of paraffin-embedded Human mammary cancer tissue, labeling NOX5 using ab191010 at 1 μg/ml.

Supplier Data

Western blot - Anti-NOX5 antibody - C-terminal (AB191010)

All lanes:

Western blot - Anti-NOX5 antibody - C-terminal (ab191010) at 0.5 µg/mL

Lane 1:

22RV1 cell lysate at 40 µg

Lane 2:

PANC cell lysate at 40 µg

Lane 3:

HeLa cell lysate at 40 µg

Lane 4:

SKOV cell lysate at 40 µg

Predicted band size: 86 kDa

Observed band size: 86 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human NADPH oxidase 5 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96PH1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-1 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOX5 also known as NADPH oxidase 5 is a member of the NADPH oxidase family which has a molecular mass of approximately 82 kDa. NOX5 is expressed widely across various tissues including the testes spleen and the fetal kidney. It is distinct because it can generate reactive oxygen species (ROS) in response to changes in intracellular calcium levels operating independently from the activation of other related NADPH oxidase enzymes.

Biological function summary

NOX5 plays a role in producing reactive oxygen species which are important for cellular signaling. The protein sometimes functions as an independent enzyme and does not typically form a complex with other proteins. Its calcium-binding domain is essential for its activity allowing it to respond dynamically to cellular changes. This ability to modulate ROS production implicates NOX5 in processes like cell proliferation migration and endothelial function.

Pathways

NOX5 contributes to cellular oxidative stress response and angiogenesis. The enzyme's activity in ROS generation links it with the MAPK signaling pathway which is important for cell differentiation and senescence. Additionally it's associated with the calcium signaling pathway interacting with proteins such as calmodulin which modulate its activity. These interactions ensure NOX5's role in balancing cellular oxidative signaling and responding to calcium fluctuations.

NOX5 is linked with hypertension and cancer. The enzyme's involvement in ROS production can lead to oxidative stress contributing to vascular dysfunction and the pathogenesis of hypertension. In cancer NOX5-related ROS generation can promote tumor growth and metastasis. It shares connections with other oxidative stress-related proteins like NOX1 and NOX2 which also have critical roles in disease progression through similar mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed : 12686516). May play a role in cell growth and apoptosis (PubMed : 12686516).. Isoform v2. Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed : 11483596, PubMed : 14982937, PubMed : 17275676, PubMed : 17587483, PubMed : 21642394, PubMed : 22387196, PubMed : 22427510, PubMed : 24505490, PubMed : 36653838). Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contributes to endothelial response to thrombin (PubMed : 17275676). Regulates redox-dependent processes in lymphocytes and spermatozoa (PubMed : 11483596).. Isoform v1. Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor.. Isoform v5. This isoform lacks calcium-binding domains and was showed to present a NADPH oxidase activity in a calcium-independent manner (PubMed : 17275676, PubMed : 36653838). May be involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin (PubMed : 17275676). However another study showed an absence of oxidase activity (PubMed : 22427510). Subject to rapid degradation (PubMed : 36653838).. Isoform v3. Lacks calcium-dependent NADPH oxidase activity.. Isoform v4. Lacks calcium-dependent NADPH oxidase activity.

See full target information NADPH oxidase 5

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of reproduction & infertility 25:184-192 PubMed39830324

2025

Association of NOX5 Expression with Sperm Activity and Motility in Pathospermic Infertile Men.

Applications

Unspecified application

Species

Unspecified reactive species

Benay Daylan,Oya Korkmaz,Olgu Enis Tok,Cagri Cakici,Turkan Yigitbasi,Ayse Karahasanoglu,Tuba Varlı Yelke,Tugba Senel Ustabas,Sule Ayla

American journal of physiology. Endocrinology and metabolism 327:E1-E12 PubMed38690939

2024

NADPH oxidase 4-derived hydrogen peroxide counterbalances testosterone-induced endothelial dysfunction and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Juliano V Alves,Rafael M da Costa,Wanessa M C Awata,Ariane Bruder-Nascimento,Shubhnita Singh,Rita C Tostes,Thiago Bruder-Nascimento

Heliyon 9:e22067 PubMed38027669

2023

Unveiling the molecular Culprit of arterial stiffness in vitamin D deficiency and obesity: Potential for novel therapeutic targets.

Applications

Unspecified application

Species

Unspecified reactive species

Adel B Elmoselhi,Amal Bouzid,Mohamed Seif Allah,Zeinab Ibrahim,Khuloud Bajbouj,Rebal S Abou Assaleh,Thenmozhi Venkatachalam,Mohamed Madkour,Ruqaiyyah Siddiqui,Naveed Ahmed Khan,Rifat A Hamoudi

Antioxidants (Basel, Switzerland) 12: PubMed36829947

2023

Sulodexide Prevents Hyperglycemia-Induced Endothelial Dysfunction and Oxidative Stress in Porcine Retinal Arterioles.

Applications

Unspecified application

Species

Unspecified reactive species

Alice Dauth,Andrzej Bręborowicz,Yue Ruan,Qi Tang,Jenia K Zadeh,Elsa W Böhm,Norbert Pfeiffer,Pratik H Khedkar,Andreas Patzak,Ksenija Vujacic-Mirski,Andreas Daiber,Adrian Gericke

International journal of cardiology 370:454-462 PubMed36332749

2022

NOX2 and NOX5 are increased in cardiac microvascular endothelium of deceased COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Jiang,Linghe Wu,Britt van der Leeden,Albert C van Rossum,Hans W M Niessen,Paul A J Krijnen

Antioxidants (Basel, Switzerland) 11: PubMed35453301

2022

Blueberry Polyphenols Increase Nitric Oxide and Attenuate Angiotensin II-Induced Oxidative Stress and Inflammatory Signaling in Human Aortic Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rami S Najjar,Shengyu Mu,Rafaela G Feresin

International journal of oncology 59: PubMed34278462

2021

NADPH oxidase 5 has a crucial role in cellular motility of colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Naoki Ashizawa,Hiroki Shimizu,Katsutoshi Shoda,Shinji Furuya,Hidenori Akaike,Naohiro Hosomura,Yoshihiko Kawaguchi,Hidetake Amemiya,Hiromichi Kawaida,Makoto Sudo,Shingo Inoue,Hiroshi Kono,Keita Katsurahara,Atsushi Shiozaki,Daisuke Ichikawa

Clinical science (London, England : 1979) 135:1845-1858 PubMed34269800

2021

Lysophosphatidylcholine induces oxidative stress in human endothelial cells via NOX5 activation - implications in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Josiane Fernandes da Silva,Juliano V Alves,Julio A Silva-Neto,Rafael M Costa,Karla B Neves,Rheure Alves-Lopes,Livia L Carmargo,Francisco J Rios,Augusto C Montezano,Rhian M Touyz,Rita C Tostes

Frontiers in cell and developmental biology 9:655794 PubMed34026754

2021

NADPH Oxidase 5 and Melatonin: Involvement in Ram Sperm Capacitation.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Miguel-Jiménez,Blanca Pina-Beltrán,Silvia Gimeno-Martos,Melissa Carvajal-Serna,Adriana Casao,Rosaura Pérez-Pe

Antioxidants (Basel, Switzerland) 10: PubMed34064498

2021

Distinction between 2'- and 3'-Phosphate Isomers of a Fluorescent NADPH Analogue Led to Strong Inhibition of Cancer Cells Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Raoul Manuel,Michelle de Souza Lima,Sébastien Dilly,Sylvain Daunay,Patricia Abbe,Elodie Pramil,Stéphanie Solier,Fabienne Guillaumond,Sarah-Simha Tubiana,Alexandre Escargueil,João Antonio Pêgas Henriques,Nathalie Ferrand,Irène Erdelmeier,Jean-Luc Boucher,Gildas Bertho,Israel Agranat,Stéphane Rocchi,Michèle Sabbah,Anny Slama Schwok

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOX5 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-NOX5 antibody - C-terminal (AB191010)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX5 antibody - C-terminal (AB191010)

Western blot - Anti-NOX5 antibody - C-terminal (AB191010)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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