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AB36833

Anti-Noxa antibody

2

(2 Reviews)

|

(17 Publications)

Rabbit Polyclonal Noxa antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human PMAIP1.

View Alternative Names

NOXA, PMAIP1, Phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, Immediate-early-response protein APR, Protein Noxa

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noxa antibody (AB36833)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noxa antibody (AB36833)

ab36833 at 1μg/ml staining Noxa in human stomach tissue by IHC

Immunocytochemistry/ Immunofluorescence - Anti-Noxa antibody (AB36833)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Noxa antibody (AB36833)

Immunofluorescence of Noxa in Human Stomach cells using ab36833 at 10 ug/ml.

Immunocytochemistry/ Immunofluorescence - Anti-Noxa antibody (AB36833)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Noxa antibody (AB36833)

ICC/IF image of ab36833 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36833, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - Anti-Noxa antibody (AB36833)
  • WB

Unknown

Western blot - Anti-Noxa antibody (AB36833)

All lanes:

Western blot - Anti-Noxa antibody (ab36833) at 0.5 µg/mL

All lanes:

Human stomach cell lysate

Predicted band size: 6 kDa

Observed band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PMAIP1. The exact immunogen used to generate this antibody is proprietary information.

Q13794

Specificity

Although immunogen is from Mouse, customer feedback indicates that the results for Mouse are non-specific (see Abreviews).

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Noxa also known as PMAIP1 is a pro-apoptotic member of the Bcl-2 protein family. It has a molecular weight of approximately 20 kDa. Noxa commonly expresses in a number of tissues including lymphoid and myeloid lineages. It functions by interacting with other Bcl-2 family proteins like Mcl-1 promoting apoptosis through binding and neutralization of anti-apoptotic proteins. This interaction facilitates the release of cytochrome c from the mitochondria triggering caspase activation and subsequent apoptosis.
Biological function summary

Noxa plays a role in the regulation of programmed cell death including being part of multiprotein complexes within the mitochondria. Noxa's expression is upregulated in response to various cellular stress signals. These stresses include DNA damage and hypoxia where it acts to mediate apoptosis preventing the survival of cells with potential mutations. Its role in modulating apoptosis makes it key in maintaining cellular homeostasis.

Pathways

Noxa is involved in significant apoptotic and cellular stress response pathways. It forms part of the intrinsic apoptosis pathway which is tightly regulated by Bcl-2 family members like BAK and BAX. Noxa's ability to neutralize Mcl-1 allows the activation of these proteins committing the cell to apoptosis. It also participates in the p53 signaling pathway which is important for the cellular response to DNA damage wherein p53 directly transactivates Noxa.

Noxa is important in cancer and autoimmune syndromes. In cancer its expression often correlates with the sensitivity of cancer cells to chemotherapy as its role in apoptosis can enhance the elimination of cancerous cells. Mutations in p53 that impair its function can lead to decreased Noxa expression and apoptosis contributing to cancer progression. In autoimmune disorders aberrant regulation of apoptosis sometimes involving Noxa and its interaction with Bcl-2 proteins can result in premature cell death or survival of autoreactive cells exacerbating the disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
See full target information PMAIP1
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:677 PubMed39285161

2024

Contribution of A1 to macrophage survival in cooperation with MCL-1 and BCL-X in a murine cell model of myeloid differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Juliane Vier,Georg Häcker,Susanne Kirschnek

Molecular therapy. Nucleic acids 18:485-495 PubMed31670198

2019

Silencing lncRNA LOC101928963 Inhibits Proliferation and Promotes Apoptosis in Spinal Cord Glioma Cells by Binding to PMAIP1.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Juan Zheng,Tian-Song Liang,Juan Wang,Jing-Yi Zhao,Dao-Ke Yang,Zhang-Suo Liu

Experimental and therapeutic medicine 18:1107-1114 PubMed31316606

2019

(3)-5,6,7-trihydroxy-3-isopropyl-3-methylisochroman-1-one inhibited osteosarcoma growth by inducing apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Zhu Song,Feng-Lin Zhang,Le-Jun Lin

Journal of cellular and molecular medicine 22:6213-6227 PubMed30255595

2018

Repression of Noxa by Bmi1 contributes to deguelin-induced apoptosis in non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Xinfang Yu,Zhenkun Xia,Xinyou Yu,Li Xie,Xiaolong Ma,Huiling Zhou,Lijun Liu,Jian Wang,Yifeng Yang,Haidan Liu

Oncotarget 9:512-523 PubMed29416632

2018

HDAC inhibitor PAC-320 induces G2/M cell cycle arrest and apoptosis in human prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixiong Dong,Yang Yang,Shuxia Liu,Jun Lu,Baiqu Huang,Yu Zhang

Cell death & disease 9:134 PubMed29374147

2018

Involvement of both caspase-8 and Noxa-activated pathways in endoplasmic reticulum stress-induced apoptosis in triple-negative breast tumor cells.

Applications

IF

Species

Unspecified reactive species

Ana Cano-González,Marta Mauro-Lizcano,Daniel Iglesias-Serret,Joan Gil,Abelardo López-Rivas

Reproductive toxicology (Elmsford, N.Y.) 68:171-190 PubMed27443218

2016

Chromium VI - Induced developmental toxicity of placenta is mediated through spatiotemporal dysregulation of cell survival and apoptotic proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Sakhila K Banu,Jone A Stanley,Kirthiram K Sivakumar,Joe A Arosh,Robert J Taylor,Robert C Burghardt

Cellular physiology and biochemistry : internation 35:2255-71 PubMed25895606

2015

Synergistic Induction of Erlotinib-Mediated Apoptosis by Resveratrol in Human Non-Small-Cell Lung Cancer Cells by Down-Regulating Survivin and Up-Regulating PUMA.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Nie,Weicheng Hu,Tao Zhang,Yiju Yang,Benxin Hou,Zhengzhi Zou

International journal of clinical and experimental 8:3811-8 PubMed26097563

2015

Combinatorial effects of miR-20a and miR-29b on neuronal apoptosis induced by spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Jun Liu,Xue-Ping Zheng,Rui Zhang,Yun-Liang Guo,Jian-Hong Wang

Neoplasia (New York, N.Y.) 15:738-48 PubMed23814486

2013

PAX3-FOXO1 induces up-regulation of Noxa sensitizing alveolar rhabdomyosarcoma cells to apoptosis.

Applications

WB

Species

Unspecified reactive species

Amy D Marshall,Fabrizio Picchione,Ramon I Klein Geltink,Gerard C Grosveld
View all publications
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