Rabbit Polyclonal Noxa antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human PMAIP1.
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- Molecular therapy. Nucleic acids 18:485-4952019Silencing lncRNA LOC101928963 Inhibits Proliferation and Promotes Apoptosis in Spinal Cord Glioma Cells by Binding to PMAIP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing-Juan Zheng et. al.PubMed 31670198
- Experimental and therapeutic medicine 18:1107-11142019(3)-5,6,7-trihydroxy-3-isopropyl-3-methylisochroman-1-one inhibited osteosarcoma growth by inducing apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Zhu Song et. al.PubMed 31316606
- Journal of cellular and molecular medicine 22:6213-62272018Repression of Noxa by Bmi1 contributes to deguelin-induced apoptosis in non-small cell lung cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Li et. al.PubMed 30255595
- Oncotarget 9:512-5232018HDAC inhibitor PAC-320 induces G2/M cell cycle arrest and apoptosis in human prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhixiong Dong,Yang Yang,Shuxia Liu,Jun Lu,Baiqu Huang,Yu ZhangPubMed 29416632
- Cell death & disease 9:1342018Involvement of both caspase-8 and Noxa-activated pathways in endoplasmic reticulum stress-induced apoptosis in triple-negative breast tumor cells.Applications:IFReactive species:Unspecified reactive speciesAna Cano-González et. al.PubMed 29374147
- Reproductive toxicology (Elmsford, N.Y.) 68:171-1902016Chromium VI - Induced developmental toxicity of placenta is mediated through spatiotemporal dysregulation of cell survival and apoptotic proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSakhila K Banu,Jone A Stanley,Kirthiram K Sivakumar,Joe A Arosh,Robert J Taylor,Robert C BurghardtPubMed 27443218
- Cellular physiology and biochemistry : internation 35:2255-712015Synergistic Induction of Erlotinib-Mediated Apoptosis by Resveratrol in Human Non-Small-Cell Lung Cancer Cells by Down-Regulating Survivin and Up-Regulating PUMA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeipei Nie et. al.PubMed 25895606
- International journal of clinical and experimental 8:3811-82015Combinatorial effects of miR-20a and miR-29b on neuronal apoptosis induced by spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue-Jun Liu et. al.PubMed 26097563
- Neoplasia (New York, N.Y.) 15:738-482013PAX3-FOXO1 induces up-regulation of Noxa sensitizing alveolar rhabdomyosarcoma cells to apoptosis.Applications:WBReactive species:Unspecified reactive speciesAmy D Marshall,Fabrizio Picchione,Ramon I Klein Geltink,Gerard C GrosveldPubMed 23814486
- The Journal of biological chemistry 288:7606-172013p53 and translation attenuation regulate distinct cell cycle checkpoints during endoplasmic reticulum (ER) stress.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PMAIP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13794
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
Alternative names
NOXA, PMAIP1, Phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, Immediate-early-response protein APR, Protein Noxa
Recommended products
Rabbit Polyclonal Noxa antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human PMAIP1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PMAIP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13794
- Purification technique
- Affinity purification Immunogen
- Specificity
Although immunogen is from Mouse, customer feedback indicates that the results for Mouse are non-specific (see Abreviews).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Noxa also known as PMAIP1 is a pro-apoptotic member of the Bcl-2 protein family. It has a molecular weight of approximately 20 kDa. Noxa commonly expresses in a number of tissues including lymphoid and myeloid lineages. It functions by interacting with other Bcl-2 family proteins like Mcl-1 promoting apoptosis through binding and neutralization of anti-apoptotic proteins. This interaction facilitates the release of cytochrome c from the mitochondria triggering caspase activation and subsequent apoptosis.
- Biological function summary
Noxa plays a role in the regulation of programmed cell death including being part of multiprotein complexes within the mitochondria. Noxa's expression is upregulated in response to various cellular stress signals. These stresses include DNA damage and hypoxia where it acts to mediate apoptosis preventing the survival of cells with potential mutations. Its role in modulating apoptosis makes it key in maintaining cellular homeostasis.
- Pathways
Noxa is involved in significant apoptotic and cellular stress response pathways. It forms part of the intrinsic apoptosis pathway which is tightly regulated by Bcl-2 family members like BAK and BAX. Noxa's ability to neutralize Mcl-1 allows the activation of these proteins committing the cell to apoptosis. It also participates in the p53 signaling pathway which is important for the cellular response to DNA damage wherein p53 directly transactivates Noxa.
- Associated diseases and disorders
Noxa is important in cancer and autoimmune syndromes. In cancer its expression often correlates with the sensitivity of cancer cells to chemotherapy as its role in apoptosis can enhance the elimination of cancerous cells. Mutations in p53 that impair its function can lead to decreased Noxa expression and apoptosis contributing to cancer progression. In autoimmune disorders aberrant regulation of apoptosis sometimes involving Noxa and its interaction with Bcl-2 proteins can result in premature cell death or survival of autoreactive cells exacerbating the disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Noxa antibody (ab36833)
All lanes: Western blot - Anti-Noxa antibody (ab36833) at 0.5 µg/mL
All lanes: Human stomach cell lysate
Predicted band size: 6 kDa
Observed band size: 15 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noxa antibody (ab36833)
ab36833 at 1μg/ml staining Noxa in human stomach tissue by IHC
Immunocytochemistry/ Immunofluorescence - Anti-Noxa antibody (ab36833)
ICC/IF image of ab36833 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36833, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-Noxa antibody (ab36833)
Immunofluorescence of Noxa in Human Stomach cells using ab36833 at 10 ug/ml.
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