AB222852

Anti-NOXA1 antibody

Name: Anti-NOXA1 antibody (ab222852) | Abcam
Brand: Abcam Limited
SKU: AB222852

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(2 Publications)

Rabbit Polyclonal NOXA1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NOXA1 aa 150-350.

View Alternative Names

P51NOX, NOXA1, NADPH oxidase activator 1, NOX activator 1, Antigen NY-CO-31, NCF2-like protein, P67phox-like factor, p51-nox

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA1 antibody (AB222852)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA1 antibody (AB222852)

Paraffin-embedded human kidney tissue stained for NOXA1 using ab222852 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NOXA1 antibody (AB222852)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NOXA1 antibody (AB222852)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for NOXA1 (green) using ab222852 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® congugated Goat Anti-Rabbit IgG (H+L).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA1 antibody (AB222852)

Paraffin-embedded human lung cancer tissue stained for NOXA1 using ab222852 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-NOXA1 antibody (AB222852)

All lanes:

Western blot - Anti-NOXA1 antibody (ab222852) at 1/1000 dilution

All lanes:

Mouse brain lysate

Secondary

All lanes:

Goat polyclonal to rabbit at 1/10000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NOXA1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q86UR1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOXA1 also known as NADPH oxidase activator 1 functions as an important regulatory component in the assembly and activation of the NADPH oxidase complex. This protein has a mass of approximately 57 kDa. It is usually expressed in various tissues including the pancreas and lung indicating its diverse role in physiological processes. NOXA1 works by facilitating the production of reactive oxygen species (ROS) through stimulating the activity of NADPH oxidase an important action for cellular signaling and defense.

Biological function summary

The NOXA1 protein interacts in the complex environment of intracellular processes. It forms part of the NADPH oxidase complex influencing ROS generation which plays a role in cell signaling microbial killing and maintaining vascular tone. Through these functions NOXA1 contributes to normal and pathological conditions by promoting oxidative stress ultimately affecting both immune responses and cellular communications.

Pathways

NOXA1 associates significantly with the superoxide production pathway through its action in NADPH oxidase complexes. It interacts closely with proteins like p67phox and p47phox important for proper complex activation and function. NOXA1 also participates in the signaling pathway related to inflammation where it modulates the immune response alongside proteins such as Rac1 and Rac2. These interactions place NOXA1 in key regulatory roles within these biological pathways.

NOXA1 links to various oxidative stress-related conditions. For example chronic granulomatous disease features dysfunctional NADPH oxidase activity in which NOXA1's proper function is compromised. Additionally NOXA1 exhibits connections to cardiovascular disorders as its role in producing reactive oxygen species can exacerbate oxidative damage contributing to disease progression. Proteins like gp91phox are also involved in these conditions highlighting NOXA1's significance in these contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Functions as an activator of NOX1, a superoxide-producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3.

See full target information NOXA1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:257 PubMed40436859

2025

NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiming Liu,Hui Lu,Xianjuan Zhang,Shuai Tang,Antao Lin,Shuo Han,Xuexiao Ma

Journal of translational medicine 23:227 PubMed40001145

2025

Revolutionizing the treatment of intervertebral disc degeneration: an approach based on molecular typing.

Applications

Unspecified application

Species

Unspecified reactive species

Shaofeng Chen,Wei Zhang,Yifan Liu,Runzhi Huang,Xiaoyi Zhou,Xianzhao Wei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOXA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA1 antibody (AB222852)

Immunocytochemistry/ Immunofluorescence - Anti-NOXA1 antibody (AB222852)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA1 antibody (AB222852)

Western blot - Anti-NOXA1 antibody (AB222852)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise