AB109366

Anti-NOXA2/p67phox antibody [EPR5064]

Name: Anti-NOXA2/p67phox antibody [EPR5064] (ab109366) | Abcam
Brand: Abcam Limited
SKU: AB109366
Price: 365 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(22 Publications)

Rabbit Recombinant Monoclonal NOXA2/p67phox antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 22 publications.

View Alternative Names

NOXA2, P67PHOX, NCF2, Neutrophil cytosol factor 2, NCF-2, 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, Neutrophil NADPH oxidase factor 2, p67-phox

2 Images

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Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (AB109366)

All lanes:

Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366) at 1/1000 dilution

Lane 1:

HL-60 cells treated with dexomethasome, dimethyl sulfoxide, and TPA at 10 µg

Lane 2:

THP-1 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 60 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] (AB109366)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] (AB109366)

Immunohistochemical analysis of NOXA2/p67phox in paraffin-embedded Human spleen tissue using ab109366 at 1/500 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-NOXA2/p67phox antibody [EPR5064]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-NOXA2/p67phox antibody [EPR5064]
Carrier free

Anti-NOXA2/p67phox antibody [EPR5064] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5064

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NOXA2 also known as p67phox is a subunit of the NADPH oxidase complex essential in producing reactive oxygen species (ROS). The protein weighs about 67 kilodaltons and is expressed in phagocytic cells like neutrophils and macrophages. NOXA2/p67phox interacts closely with other subunits of the NADPH oxidase complex playing a critical role in oxidative burst which is essential in the immune response against pathogens.

Biological function summary

NOXA2 contributes to superoxide production by facilitating electron transfer in the NADPH oxidase complex. This complex is integral to the immune system's ability to target and destroy infectious agents. NOXA2/p67phox forms a complex with other components such as gp91phox and p22phox which are required for full activation of the oxidase. The coordinated action of these complexes ensures effective ROS production which is a vital component of the defense mechanism of immune cells.

Pathways

NOXA2 participates in the respiratory burst pathway an important element of innate immunity. This pathway involves the conversion of oxygen to superoxide a process requiring precise interaction with cytochrome b558 and the Rac protein. NOXA2 is also related to signal transduction pathways including those modulated by protein kinase C which further regulates its activation during the immune response. The orchestration of these pathways allows for an effective immune defense mechanism.

NOXA2 is implicated in chronic granulomatous disease (CGD) where mutations in the components of the NADPH oxidase complex impair ROS production. This leads to frequent infections due to deficient pathogen clearance. NOXA2 is also linked to inflammatory disorders due to its role in excessive ROS production. In that context it connects with other proteins such as p47phox which are also involved in both normal and aberrant immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

See full target information NCF2

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Phytotherapy research : PTR 38:5441-5457 PubMed39289784

2024

Geniposidic Acid Attenuates Chronic Tubulointerstitial Nephropathy Through Regulation of the NF-ƙB/Nrf2 Pathway Via Aryl Hydrocarbon Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ni Wang,Xiao-Jun Li,Wen-Feng Wang,Liang Zou,Hua Miao,Ying-Yong Zhao

Nature communications 15:6218 PubMed39043696

2024

Dissemination of pathogenic bacteria is reinforced by a MARTX toxin effector duet.

Applications

Unspecified application

Species

Unspecified reactive species

Sanghyeon Choi,Youngjin Lee,Shinhye Park,Song Yee Jang,Jongbin Park,Do Won Oh,Su-Man Kim,Tae-Hwan Kim,Ga Seul Lee,Changyi Cho,Byoung Sik Kim,Donghan Lee,Eun-Hee Kim,Hae-Kap Cheong,Jeong Hee Moon,Ji-Joon Song,Jungwon Hwang,Myung Hee Kim

Nature communications 15:5170 PubMed38886341

2024

NCF4 attenuates colorectal cancer progression by modulating inflammasome activation and immune surveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Longjun Li,Rudi Mao,Shenli Yuan,Qingqing Xie,Jinyu Meng,Yu Gu,Siyu Tan,Xiaoqing Xu,Chengjiang Gao,Hongbin Liu,Chunhong Ma,Si Ming Man,Xiangbo Meng,Tao Xu,Xiaopeng Qi

Journal of radiation research 65:291-302 PubMed38588586

2024

Hydrogen-rich solution alleviates acute radiation pneumonitis by regulating oxidative stress and macrophages polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yin,Wenjing Xu,Junjun Ling,Lihai Ma,Hao Zhang,Pei Wang

Renal failure 45:2199095 PubMed37038746

2023

Bioinformatics analysis of potential key ferroptosis-related genes involved in tubulointerstitial injury in patients with diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Li Ma,Yu Bai,Wen-Hu Liu,Zong-Li Diao

Cancer medicine 12:8991-9004 PubMed36680322

2023

Role of NCF2 as a potential prognostic factor and immune infiltration indicator in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Huang,Jing Zhang,Shuwen Kuang,Zhuo Li,Hong Zhao,Jianxiong Wu,Mei Liu,Liming Wang

Frontiers in pharmacology 12:800522 PubMed35002735

2021

Shenkang Injection and Its Three Anthraquinones Ameliorates Renal Fibrosis by Simultaneous Targeting IƙB/NF-ƙB and Keap1/Nrf2 Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Liang-Pu Luo,Ping Suo,Li-Li Ren,Hong-Jiao Liu,Yamei Zhang,Ying-Yong Zhao

Redox biology 30:101432 PubMed31986467

2020

Enhanced cardiomyocyte reactive oxygen species signaling promotes ibrutinib-induced atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Yang,Na An,Changming Zhong,Manke Guan,Yuchen Jiang,Xinye Li,Hanlai Zhang,Liqin Wang,Yanfei Ruan,Yonghong Gao,Nian Liu,Hongcai Shang,Yanwei Xing

The Journal of pharmacology and experimental thera 371:348-359 PubMed31467086

2019

Indigo Fruits Ingredient, Aucubin, Protects against LPS-Induced Cardiac Dysfunction in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

MingXia Duan,Yuan Yuan,Chen Liu,Zhulan Cai,Qingwen Xie,Tongtong Hu,Qizhu Tang,QingQing Wu

PLoS pathogens 15:e1008004 PubMed31412082

2019

Fas-associated factor 1 mediates NADPH oxidase-induced reactive oxygen species production and proinflammatory responses in macrophages against Listeria infection.

Applications

Unspecified application

Species

Unspecified reactive species

Tae-Hwan Kim,Hyun-Cheol Lee,Jae-Hoon Kim,C Y Hewawaduge,Kiramage Chathuranga,W A Gayan Chathuranga,Pathum Ekanayaka,H M S M Wijerathne,Chul-Joong Kim,Eunhee Kim,Jong-Soo Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NOXA2/p67phox antibody [EPR5064]

Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (AB109366)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] (AB109366)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

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