Rabbit Recombinant Monoclonal NOXA2/p67phox antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 16 publications.
Images
![Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (AB109366), expandable thumbnail](https://content.abcam.com/products/images/noxa2-p67phox-antibody-epr5064-ab109366--western-blot-img128069.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (AB109366)")
Publications
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- Renal failure 45:21990952023Bioinformatics analysis of potential key ferroptosis-related genes involved in tubulointerstitial injury in patients with diabetic nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Li Ma,Yu Bai,Wen-Hu Liu,Zong-Li DiaoPubMed 37038746
- Cancer medicine 12:8991-90042023Role of NCF2 as a potential prognostic factor and immune infiltration indicator in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Huang,Jing Zhang,Shuwen Kuang,Zhuo Li,Hong Zhao,Jianxiong Wu,Mei Liu,Liming WangPubMed 36680322
- Frontiers in pharmacology 12:8005222021Shenkang Injection and Its Three Anthraquinones Ameliorates Renal Fibrosis by Simultaneous Targeting IƙB/NF-ƙB and Keap1/Nrf2 Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang-Pu Luo,Ping Suo,Li-Li Ren,Hong-Jiao Liu,Yamei Zhang,Ying-Yong ZhaoPubMed 35002735
- Redox biology 30:1014322020Enhanced cardiomyocyte reactive oxygen species signaling promotes ibrutinib-induced atrial fibrillation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyu Yang et. al.PubMed 31986467
- The Journal of pharmacology and experimental thera 371:348-3592019Indigo Fruits Ingredient, Aucubin, Protects against LPS-Induced Cardiac Dysfunction in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingXia Duan et. al.PubMed 31467086
- PLoS pathogens 15:e10080042019Fas-associated factor 1 mediates NADPH oxidase-induced reactive oxygen species production and proinflammatory responses in macrophages against Listeria infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTae-Hwan Kim,Hyun-Cheol Lee,Jae-Hoon Kim,C Y Hewawaduge,Kiramage Chathuranga,W A Gayan Chathuranga,Pathum Ekanayaka,H M S M Wijerathne,Chul-Joong Kim,Eunhee Kim,Jong-Soo LeePubMed 31412082
- Frontiers in pharmacology 10:4312019Interaction Mechanisms Between the NOX4/ROS and RhoA/ROCK1 Signaling Pathways as New Anti- fibrosis Targets of Ursolic Acid in Hepatic Stellate Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenkai Huang et. al.PubMed 31130857
- Frontiers in pharmacology 10:3812019YiQiFuMai Powder Injection Attenuates Coronary Artery Ligation-Induced Heart Failure Through Improving Mitochondrial Function via Regulating ROS Generation and CaMKII Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Zhang,Ling Zhang,Yan Zhang,Xiaoxue Fan,Weiwei Yang,Boyang Yu,Junping Kou,Fang LiPubMed 31031629
- Molecular nutrition & food research 63:e18010422019Antihypertensive Potential of Coenzyme Q10 via Free Radical Scavenging and Enhanced Akt-nNOS Signaling in the Nucleus Tractus Solitarii in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHsin-Hung Chen et. al.PubMed 30668894
- Frontiers in physiology 9:652018TNF-α Increases Production of Reactive Oxygen Species through Cdk5 Activation in Nociceptive Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRodrigo Sandoval et. al.PubMed 29467671
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Alternative names
Neutrophil cytosol factor 2, NCF-2, 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, Neutrophil NADPH oxidase factor 2, p67-phox, P67PHOX, NOXA2, NCF2
Recommended products
Rabbit Recombinant Monoclonal NOXA2/p67phox antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 16 publications.
Alternative names
Neutrophil cytosol factor 2, NCF-2, 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, Neutrophil NADPH oxidase factor 2, p67-phox, P67PHOX, NOXA2, NCF2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR5064
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NOXA2 also known as p67phox is a subunit of the NADPH oxidase complex essential in producing reactive oxygen species (ROS). The protein weighs about 67 kilodaltons and is expressed in phagocytic cells like neutrophils and macrophages. NOXA2/p67phox interacts closely with other subunits of the NADPH oxidase complex playing a critical role in oxidative burst which is essential in the immune response against pathogens.
- Biological function summary
NOXA2 contributes to superoxide production by facilitating electron transfer in the NADPH oxidase complex. This complex is integral to the immune system's ability to target and destroy infectious agents. NOXA2/p67phox forms a complex with other components such as gp91phox and p22phox which are required for full activation of the oxidase. The coordinated action of these complexes ensures effective ROS production which is a vital component of the defense mechanism of immune cells.
- Pathways
NOXA2 participates in the respiratory burst pathway an important element of innate immunity. This pathway involves the conversion of oxygen to superoxide a process requiring precise interaction with cytochrome b558 and the Rac protein. NOXA2 is also related to signal transduction pathways including those modulated by protein kinase C which further regulates its activation during the immune response. The orchestration of these pathways allows for an effective immune defense mechanism.
- Associated diseases and disorders
NOXA2 is implicated in chronic granulomatous disease (CGD) where mutations in the components of the NADPH oxidase complex impair ROS production. This leads to frequent infections due to deficient pathogen clearance. NOXA2 is also linked to inflammatory disorders due to its role in excessive ROS production. In that context it connects with other proteins such as p47phox which are also involved in both normal and aberrant immune responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366), expandable thumbnail](https://content.abcam.com/products/images/noxa2-p67phox-antibody-epr5064-ab109366--western-blot-img128069.jpg "Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)")
Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)
All lanes: Western blot - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366) at 1/1000 dilution
Lane 1: HL-60 cells treated with dexomethasome, dimethyl sulfoxide, and TPA at 10 µg
Lane 2: THP-1 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366), expandable thumbnail](https://content.abcam.com/products/images/noxa2-p67phox-antibody-epr5064-ab109366--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87757.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5064] (ab109366)
Immunohistochemical analysis of NOXA2/p67phox in paraffin-embedded Human spleen tissue using ab109366 at 1/500 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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