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Rabbit Recombinant Monoclonal NP-I antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Transfected cell line, Recombinant full length protein - Human samples.

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Images

Immunoprecipitation - Anti-NP-I antibody [EPR25683-17] (AB300404), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-17] (AB300404), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NP-I antibody [EPR25683-17] (AB300404), expandable thumbnail
  • Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404), expandable thumbnail
  • Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Tested
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant full length protein - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Transfected cell line
Not recommended
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Transfected cell line, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Transfected cell line, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Transfected cell line, Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human, Transfected cell line, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Recombinant full length protein - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Target data

Function

May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.

Alternative names

Neuronal pentraxin-1, NP1, Neuronal pentraxin I, NP-I, NPTX1

Recommended products

Rabbit Recombinant Monoclonal NP-I antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Transfected cell line, Recombinant full length protein - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25683-17
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NP-I also known as NP protein or NP-BSA functions as a nucleoprotein component of ribonucleoprotein complexes. It has a mass of 56 kDa. Expression occurs in both the cytoplasm and nucleus of infected cells. Ribonucleoproteins with NP-I encapsulate viral RNA which helps in the process of viral replication and transcription. NP-I is essential for forming the ribonucleoprotein core stabilizing the viral genome and facilitating interactions with the polymerase.

Biological function summary

NP-I plays important roles in the life cycle of certain viruses. It associates with NP-TX1 another protein component to ensure effective assembly of the viral replication machinery. NP-I is also part of a complex that maintains the integrity of the viral RNA. It modulates host cell responses to viral infection by interacting with host cellular proteins impacting viral pathogenicity and replication efficiency.

Pathways

NP-I interacts with the RNA replication and transcription processes of viruses. It participates in the influenza virus life cycle pathway where NP protein coordinates with polymerase subunits like PB1 PB2 and PA to facilitate RNA synthesis. These interactions and pathways are critical for understanding how viruses exploit host cellular machinery to perpetuate their replication cycles. Specifically NP-I contributes to the assembly and regulation of viral RNA genomes within host cells.

Associated diseases and disorders

NP-I is connected to viral infections like influenza. It has a pivotal role in facilitating viral replication and immune evasion influencing the severity of influenza manifestations. The interaction between NP-I and host proteins like interferon regulators can modulate immune responses potentially leading to immune system disorders. By understanding these interactions scientists can develop therapeutic interventions targeting NP-I to control or mitigate influenza viral infections.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-NP-I antibody [EPR25683-17] (ab300404), expandable thumbnail

    Immunoprecipitation - Anti-NP-I antibody [EPR25683-17] (ab300404)

    NPTX1 was immunoprecipitated from 0.35 mg Human hypothalamus tissue lysate 10 ug with ab300404 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300404 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human hypothalamus tissue lysate 10 ug

    Lane 2: abAB300404 IP in Human hypothalamus tissue lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of abXXX in Human hypothalamus tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3.25 seconds

    All lanes: Immunoprecipitation - Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

    All lanes: Human hypothalamus tissue lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3.25s

  • Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-17] (ab300404), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-17] (ab300404)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling NPTX1 with ab300404 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-NP-I antibody [EPR25683-17] (ab300404), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NP-I antibody [EPR25683-17] (ab300404)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized IMR-32 (human neuroblastoma neuroblast) cells labelling NPTX1 with ab300404 at 1/50 (9.56 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasm staining in IMR-32 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404), expandable thumbnail

    Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

    Lane 1: His-tagged human NPTX1 recombinant protein

    Lane 2: His-tagged human NPTX2 recombinant protein

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 50 kDa

    Exposure time: 3.25s

  • Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404), expandable thumbnail

    Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

    All lanes: IMR-32 (Human neuroblastoma neuroblast) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 50 kDa

    Exposure time: 180s

  • Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404), expandable thumbnail

    Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

    Lane 1: Human hypothalamus tissue lysate

    Lane 2: Human heart tissue lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 50 kDa

    Exposure time: 10s

  • Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404), expandable thumbnail

    Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

    Lane 1: Hela (Human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate

    Lane 2: Hela transfected with siRNA specifically targeti NPTX1, whole cell lysate 20

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 50 kDa

    Exposure time: 169s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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