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AB300404

Anti-NP-I antibody [EPR25683-17]

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Rabbit Recombinant Monoclonal NP-I antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Transfected cell line, Recombinant full length protein - Human samples.

View Alternative Names

Neuronal pentraxin-1, NP1, Neuronal pentraxin I, NP-I, NPTX1

7 Images
Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-17] (AB300404)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling NPTX1 with ab300404 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NP-I antibody [EPR25683-17] (AB300404)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized IMR-32 (human neuroblastoma neuroblast) cells labelling NPTX1 with ab300404 at 1/50 (9.56 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasm staining in IMR-32 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Immunoprecipitation - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • IP

Supplier Data

Immunoprecipitation - Anti-NP-I antibody [EPR25683-17] (AB300404)

NPTX1 was immunoprecipitated from 0.35 mg Human hypothalamus tissue lysate 10 ug with ab300404 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300404 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Human hypothalamus tissue lysate 10 ug

Lane 2 : ab300404 IP in Human hypothalamus tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300404 in Human hypothalamus tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3.25 seconds

All lanes:

Immunoprecipitation - Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

All lanes:

Human hypothalamus tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3.25s

Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • WB

Supplier Data

Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

Lane 1:

Hela (Human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate

Lane 2:

Hela transfected with siRNA specifically targeti NPTX1, whole cell lysate 20

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa

false

Exposure time: 169s

Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • WB

Supplier Data

Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate

Lane 2:

Human heart tissue lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa

false

Exposure time: 10s

Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • WB

Supplier Data

Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

All lanes:

IMR-32 (Human neuroblastoma neuroblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa

false

Exposure time: 180s

Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)
  • WB

Supplier Data

Western blot - Anti-NP-I antibody [EPR25683-17] (AB300404)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-NP-I antibody [EPR25683-17] (ab300404) at 1/1000 dilution

Lane 1:

His-tagged human NPTX1 recombinant protein

Lane 2:

His-tagged human NPTX2 recombinant protein

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa

false

Exposure time: 3.25s

  • Carrier free

    Anti-NP-I antibody [EPR25683-17] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25683-17

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Transfected cell line": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NP-I also known as NP protein or NP-BSA functions as a nucleoprotein component of ribonucleoprotein complexes. It has a mass of 56 kDa. Expression occurs in both the cytoplasm and nucleus of infected cells. Ribonucleoproteins with NP-I encapsulate viral RNA which helps in the process of viral replication and transcription. NP-I is essential for forming the ribonucleoprotein core stabilizing the viral genome and facilitating interactions with the polymerase.
Biological function summary

NP-I plays important roles in the life cycle of certain viruses. It associates with NP-TX1 another protein component to ensure effective assembly of the viral replication machinery. NP-I is also part of a complex that maintains the integrity of the viral RNA. It modulates host cell responses to viral infection by interacting with host cellular proteins impacting viral pathogenicity and replication efficiency.

Pathways

NP-I interacts with the RNA replication and transcription processes of viruses. It participates in the influenza virus life cycle pathway where NP protein coordinates with polymerase subunits like PB1 PB2 and PA to facilitate RNA synthesis. These interactions and pathways are critical for understanding how viruses exploit host cellular machinery to perpetuate their replication cycles. Specifically NP-I contributes to the assembly and regulation of viral RNA genomes within host cells.

NP-I is connected to viral infections like influenza. It has a pivotal role in facilitating viral replication and immune evasion influencing the severity of influenza manifestations. The interaction between NP-I and host proteins like interferon regulators can modulate immune responses potentially leading to immune system disorders. By understanding these interactions scientists can develop therapeutic interventions targeting NP-I to control or mitigate influenza viral infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.
See full target information NPTX1_HUMAN
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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