AB289966

Anti-NP-I antibody [EPR25683-64]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal NP-I antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat, Recombinant fragment - Human samples.

View Alternative Names

Neuronal pentraxin-1, NP1, Neuronal pentraxin I, NP-I, NPTX1

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] (AB289966)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] (AB289966)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling NP-I with ab289966 at 1/2000 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab289966 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS instead of primary antibody, followed by LeicaDS9800 (Bond™, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] (AB289966)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling NP-I with ab289966 at 1/2000 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Negative control : no staining on human heart. The section was incubated with ab289966 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-64] (AB289966)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-64] (AB289966)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling NP-I with ab289966 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Lab

Immunoprecipitation - Anti-NP-I antibody [EPR25683-64] (AB289966)

NP-I was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab2568364 at 1/30 dilution (2ug in 0.07mg lysates). Western blot was performed on the immunoprecipitate using anti npi antibody epr2568364 immunoprecipitation hela.jpg at 1/5000 dilution. Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific was used at 1/100000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 2ug

Lane 2 : ab2568364 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289966 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3.25 seconds

The 38KDa band might be NPTX1 fragmemts (PMID : 29501530).

All lanes:

Immunoprecipitation - Anti-NP-I antibody [EPR25683-64] (ab289966)

Predicted band size: 47 kDa

Observed band size: 38 kDa,50 kDa

false

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] (AB289966)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling NP-I with ab289966 at 1/2000 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab289966 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS instead of primary antibody, followed by LeicaDS9800 (Bond™, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] (AB289966)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling NP-I with ab289966 at 1/2000 followed by LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with ab289966 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS instead of primary antibody, followed by LeicaDS9800 (Bond™, Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Immunoprecipitation - Anti-NP-I antibody [EPR25683-64] (AB289966)

NP-I was immunoprecipitated from Mouse hypothalamus tissue lysate with ab289966 at 1/30 dilution (2ug in 0.175mg lysates). Western blot was performed on the immunoprecipitate using ab289966 at 1/5000 dilution. Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific was used at 1/100000 dilution.

Lane 1 : Mouse hypothalamus tissue lysate 5 ug

Lane 2 : ab289966 IP in Mouse hypothalamus tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289966 in Mouse hypothalamus tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3.25 seconds

The 38KDa band might be NPTX1 fragmemts (PMID : 29501530).

All lanes:

Immunoprecipitation - Anti-NP-I antibody [EPR25683-64] (ab289966)

Predicted band size: 47 kDa

Observed band size: 38 kDa,50 kDa

false

Lab

Western blot - Anti-NP-I antibody [EPR25683-64] (AB289966)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 10 seconds

All lanes:

Western blot - Anti-NP-I antibody [EPR25683-64] (ab289966) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 16 µg

Lane 2:

HeLa transfected with siRNA specifically targeti NPTX1 whole cell lysate at 16 µg

Secondary

Lanes 1 - 2:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-NP-I antibody [EPR25683-64] (AB289966)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

NPTX1 is a glycosylated protein and can be deglycosylated by Protein Deglycosylation MIX II.

Negative control : human heart (PMID : 7695898)

Exposure time : 7.75 seconds

All lanes:

Western blot - Anti-NP-I antibody [EPR25683-64] (ab289966) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Lane 3:

Untreated Human hypothalamus tissue lysate at 20 µg

Lane 4:

Human hypothalamus tissue lysate treated with Protein Deglycosylation MIX II at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 3 - 4:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa,50 kDa

false

Lab

Western blot - Anti-NP-I antibody [EPR25683-64] (AB289966)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 29501530)

Exposure time : 15 seconds.

All lanes:

Western blot - Anti-NP-I antibody [EPR25683-64] (ab289966) at 1/1000 dilution

Lane 1:

Mouse hypothalamus tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat striatum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 38 kDa,50 kDa

false

Lab

Western blot - Anti-NP-I antibody [EPR25683-64] (AB289966)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure times : Lane 1 : 1 seconds; Lane 2 : 180 seconds

All lanes:

Western blot - Anti-NP-I antibody [EPR25683-64] (ab289966) at 1/1000 dilution

Lane 1:

His-tagged human NPTX1 recombinant protein, 10 ng

Lane 2:

His-tagged human NPTX2 recombinant protein, 10 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

Carrier free

Anti-NP-I antibody [EPR25683-64] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25683-64

Isotype

IgG

Carrier free

Reacts with

Rat, Mouse, Human

Applications

Flow Cyt (Intra), IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not react with Rat species for FC

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "Not suitable with Rat.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "Not suitable with Rat.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NP-I also known as NP protein or NP-BSA functions as a nucleoprotein component of ribonucleoprotein complexes. It has a mass of 56 kDa. Expression occurs in both the cytoplasm and nucleus of infected cells. Ribonucleoproteins with NP-I encapsulate viral RNA which helps in the process of viral replication and transcription. NP-I is essential for forming the ribonucleoprotein core stabilizing the viral genome and facilitating interactions with the polymerase.

Biological function summary

NP-I plays important roles in the life cycle of certain viruses. It associates with NP-TX1 another protein component to ensure effective assembly of the viral replication machinery. NP-I is also part of a complex that maintains the integrity of the viral RNA. It modulates host cell responses to viral infection by interacting with host cellular proteins impacting viral pathogenicity and replication efficiency.

Pathways

NP-I interacts with the RNA replication and transcription processes of viruses. It participates in the influenza virus life cycle pathway where NP protein coordinates with polymerase subunits like PB1 PB2 and PA to facilitate RNA synthesis. These interactions and pathways are critical for understanding how viruses exploit host cellular machinery to perpetuate their replication cycles. Specifically NP-I contributes to the assembly and regulation of viral RNA genomes within host cells.

NP-I is connected to viral infections like influenza. It has a pivotal role in facilitating viral replication and immune evasion influencing the severity of influenza manifestations. The interaction between NP-I and host proteins like interferon regulators can modulate immune responses potentially leading to immune system disorders. By understanding these interactions scientists can develop therapeutic interventions targeting NP-I to control or mitigate influenza viral infections.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.

See full target information NPTX1_HUMAN

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NP-I antibody [EPR25683-64]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] (AB289966)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] (AB289966)

Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-64] (AB289966)

Immunoprecipitation - Anti-NP-I antibody [EPR25683-64] (AB289966)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] (AB289966)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] (AB289966)

Immunoprecipitation - Anti-NP-I antibody [EPR25683-64] (AB289966)

Western blot - Anti-NP-I antibody [EPR25683-64] (AB289966)

Western blot - Anti-NP-I antibody [EPR25683-64] (AB289966)

Western blot - Anti-NP-I antibody [EPR25683-64] (AB289966)

Western blot - Anti-NP-I antibody [EPR25683-64] (AB289966)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise