Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)

Rabbit Recombinant Monoclonal NP-I antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat, Recombinant fragment - Human samples.

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Images

Immunoprecipitation - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (AB289990), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	Flow Cyt (Intra)	IHC-P
Human	Not recommended	Tested	Tested	Tested	Tested
Mouse	Not recommended	Tested	Tested	Expected	Tested
Rat	Not recommended	Expected	Tested	Not recommended	Tested
Recombinant fragment - Human	Not recommended	Not recommended	Tested	Not recommended	Not recommended

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human, Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Not suitable with Rat.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Not suitable with Rat.
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Target data

See full target information NPTX1_HUMAN

Function

May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.

Alternative names

Neuronal pentraxin-1, NP1, Neuronal pentraxin I, NP-I, NPTX1

Recommended products

Rabbit Recombinant Monoclonal NP-I antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat, Recombinant fragment - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR25683-64
Purification technique: Affinity purification Protein A
Specificity: This antibody does not react with Rat species for Flow cytometry.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab289990 is the carrier-free version of Anti-NP-I antibody [EPR25683-64] ab289966.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NP-I also known as NP protein or NP-BSA functions as a nucleoprotein component of ribonucleoprotein complexes. It has a mass of 56 kDa. Expression occurs in both the cytoplasm and nucleus of infected cells. Ribonucleoproteins with NP-I encapsulate viral RNA which helps in the process of viral replication and transcription. NP-I is essential for forming the ribonucleoprotein core stabilizing the viral genome and facilitating interactions with the polymerase.

Biological function summary

NP-I plays important roles in the life cycle of certain viruses. It associates with NP-TX1 another protein component to ensure effective assembly of the viral replication machinery. NP-I is also part of a complex that maintains the integrity of the viral RNA. It modulates host cell responses to viral infection by interacting with host cellular proteins impacting viral pathogenicity and replication efficiency.

Pathways

NP-I interacts with the RNA replication and transcription processes of viruses. It participates in the influenza virus life cycle pathway where NP protein coordinates with polymerase subunits like PB1 PB2 and PA to facilitate RNA synthesis. These interactions and pathways are critical for understanding how viruses exploit host cellular machinery to perpetuate their replication cycles. Specifically NP-I contributes to the assembly and regulation of viral RNA genomes within host cells.

Associated diseases and disorders

NP-I is connected to viral infections like influenza. It has a pivotal role in facilitating viral replication and immune evasion influencing the severity of influenza manifestations. The interaction between NP-I and host proteins like interferon regulators can modulate immune responses potentially leading to immune system disorders. By understanding these interactions scientists can develop therapeutic interventions targeting NP-I to control or mitigate influenza viral infections.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Immunoprecipitation - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)
This data was developed using Anti-NP-I antibody [EPR25683-64] ab289966, the same antibody clone in a different buffer formulation.
NP-I was immunoprecipitated from Mouse hypothalamus tissue lysate with Anti-NP-I antibody [EPR25683-64] ab289966 at 1/30 dilution (2ug in 0.175mg lysates). Western blot was performed on the immunoprecipitate using Anti-NP-I antibody [EPR25683-64] ab289966 at 1/5000 dilution. Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific was used at 1/100000 dilution.
Lane 1: Mouse hypothalamus tissue lysate 5 ug
Lane 2: abab289966 IP in Mouse hypothalamus tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NP-I antibody [EPR25683-64] ab289966 in Mouse hypothalamus tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds
The 38KDa band might be NPTX1 fragmemts (PMID: 29501530).
All lanes: Immunoprecipitation - Anti-NP-I antibody [EPR25683-64] (Anti-NP-I antibody [EPR25683-64] ab289966)
Predicted band size: 47 kDa
Observed band size: 38 kDa, 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)
This data was developed using Anti-NP-I antibody [EPR25683-64] ab289966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling NP-I with Anti-NP-I antibody [EPR25683-64] ab289966 at 1/2000 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with Anti-NP-I antibody [EPR25683-64] ab289966 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS instead of primary antibody, followed by LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunoprecipitation - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)
This data was developed using ab289990, the same antibody clone in a different buffer formulation.
NP-I was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab2568364 at 1/30 dilution (2ug in 0.07mg lysates). Western blot was performed on the immunoprecipitate using anti npi antibody epr2568364 immunoprecipitation hela.jpg at 1/5000 dilution. Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific was used at 1/100000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 2ug
Lane 2: ab2568364 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NP-I antibody [EPR25683-64] ab289966 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds
The 38KDa band might be NPTX1 fragmemts (PMID: 29501530).
All lanes: Immunoprecipitation - Anti-NP-I antibody [EPR25683-64] (Anti-NP-I antibody [EPR25683-64] ab289966)
Predicted band size: 47 kDa
Observed band size: 38 kDa, 50 kDa
Flow Cytometry (Intracellular) - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)
This data was developed using Anti-NP-I antibody [EPR25683-64] ab289966, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling NP-I with Anti-NP-I antibody [EPR25683-64] ab289966 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)
This data was developed using Anti-NP-I antibody [EPR25683-64] ab289966, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29501530)
Exposure time: 15 seconds.
All lanes: Western blot - Anti-NP-I antibody [EPR25683-64] (Anti-NP-I antibody [EPR25683-64] ab289966) at 1/1000 dilution
Lane 1: Mouse hypothalamus tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat striatum tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 38 kDa, 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)
This data was developed using Anti-NP-I antibody [EPR25683-64] ab289966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling NP-I with Anti-NP-I antibody [EPR25683-64] ab289966 at 1/2000 followed by LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with Anti-NP-I antibody [EPR25683-64] ab289966 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS instead of primary antibody, followed by LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)
This data was developed using Anti-NP-I antibody [EPR25683-64] ab289966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling NP-I with Anti-NP-I antibody [EPR25683-64] ab289966 at 1/2000 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with Anti-NP-I antibody [EPR25683-64] ab289966 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS instead of primary antibody, followed by LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)
This data was developed using Anti-NP-I antibody [EPR25683-64] ab289966, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure times: Lane 1: 1 seconds; Lane 2: 180 seconds
All lanes: Western blot - Anti-NP-I antibody [EPR25683-64] (Anti-NP-I antibody [EPR25683-64] ab289966) at 1/1000 dilution
Lane 1: His-tagged human NPTX1 recombinant protein, 10 ng
Lane 2: His-tagged human NPTX2 recombinant protein, 10 ng
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa
Western blot - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)
This data was developed using Anti-NP-I antibody [EPR25683-64] ab289966, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds
All lanes: Western blot - Anti-NP-I antibody [EPR25683-64] (Anti-NP-I antibody [EPR25683-64] ab289966) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 16 µg
Lane 2: HeLa transfected with siRNA specifically targeti NPTX1 whole cell lysate at 16 µg
Secondary
Lanes 1 - 2: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution
Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa
Western blot - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)
This data was developed using Anti-NP-I antibody [EPR25683-64] ab289966, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
NPTX1 is a glycosylated protein and can be deglycosylated by Protein Deglycosylation MIX II.
Negative control: human heart (PMID:7695898)
Exposure time: 7.75 seconds
All lanes: Western blot - Anti-NP-I antibody [EPR25683-64] (Anti-NP-I antibody [EPR25683-64] ab289966) at 1/1000 dilution
Lane 1: Human hypothalamus tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
Lane 3: Untreated Human hypothalamus tissue lysate at 20 µg
Lane 4: Human hypothalamus tissue lysate treated with Protein Deglycosylation MIX II at 20 µg
Secondary
Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lanes 3 - 4: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa, 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NP-I antibody [EPR25683-64] - BSA and Azide free (ab289990)
This data was developed using Anti-NP-I antibody [EPR25683-64] ab289966, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling NP-I with Anti-NP-I antibody [EPR25683-64] ab289966 at 1/2000 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Negative control: no staining on human heart. The section was incubated with Anti-NP-I antibody [EPR25683-64] ab289966 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.