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AB254662

Anti-NPAS3 antibody

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Rabbit Polyclonal NPAS3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NPAS3 aa 150-300.

View Alternative Names

BHLHE12, MOP6, PASD6, NPAS3, Neuronal PAS domain-containing protein 3, Neuronal PAS3, Basic-helix-loop-helix-PAS protein MOP6, Class E basic helix-loop-helix protein 12, Member of PAS protein 6, PAS domain-containing protein 6, bHLHe12

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAS3 antibody (AB254662)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAS3 antibody (AB254662)

Paraffin-embedded human liver tissue stained for NPAS3 using ab254662 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAS3 antibody (AB254662)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAS3 antibody (AB254662)

Paraffin-embedded human cerebral cortex tissue stained for NPAS3 using ab254662 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NPAS3 antibody (AB254662)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NPAS3 antibody (AB254662)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling NPAS3 using ab254662 at 4µg/ml (green) in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NPAS3 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q8IXF0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NPAS3 also known as Neuronal PAS domain protein 3 is a transcription factor with a mass of approximately 105 kDa. It contains basic helix-loop-helix (bHLH) DNA-binding domains and PAS domains. NPAS3 expresses mainly in the brain indicating its involvement in neurological functions. This protein acts by regulating the transcription of specific genes necessary for brain development and function.
Biological function summary

NPAS3 influences brain development and synaptic plasticity. It forms part of a transcriptional complex with other cofactors that regulates gene expression important for neurological development. NPAS3 interacts with components in the brain's signaling pathways affecting neuronal growth and responses. The protein plays a significant role in processes like neurogenesis and the maintenance of neuronal circuitry.

Pathways

NPAS3 interacts with critical signaling pathways involved in neurological development such as the Wnt signaling pathway and Notch signaling pathway. The protein serves functions in the neuronal development stage by coordinating with other pathway proteins such as β-catenin in the Wnt signaling. NPAS3 modifies transcription rates of genes involved in these pathways impacting overall brain function and development.

NPAS3 associates with psychiatric conditions and developmental disorders such as schizophrenia and bipolar disorder. Alterations in NPAS3 function or mutation have shown links with these disorders. The protein interacts with DISC1 (Disrupted-In-Schizophrenia 1) and may influence or modify its role in disease mechanisms. Understanding NPAS3's involvement can help in identifying potential therapeutic targets for neurological and psychiatric conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a broad role in neurogenesis. May control regulatory pathways relevant to schizophrenia and to psychotic illness (By similarity).
See full target information NPAS3
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Product promise

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