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AB99436

Anti-NPAT antibody

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(2 Publications)

Rabbit Polyclonal NPAT antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NPAT aa 700-750.

View Alternative Names

CAND3, E14, NPAT, Protein NPAT, Nuclear protein of the ataxia telangiectasia mutated locus, p220, Nuclear protein of the ATM locus

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAT antibody (AB99436)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPAT antibody (AB99436)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling NPAT with ab99436 at 1/1000 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-NPAT antibody (AB99436)
  • IP

Unknown

Immunoprecipitation - Anti-NPAT antibody (AB99436)

Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab99436 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane Western Blot : an antibody that recognizes a downstream epitope of NPAT at 1 µg/ml

developed using the ECL technique

Exposure time : 30 seconds

Predicted band size : 154 kDa

All lanes:

Immunoprecipitation - Anti-NPAT antibody (ab99436)

Predicted band size: 154 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human NPAT aa 700-750. The exact immunogen used to generate this antibody is proprietary information.

Q14207

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NPAT also known as nuclear protein ataxia-telangiectasia locus is a protein that weighs approximately 220 kDa. It is widely present in various tissues with a high expression in proliferating cells. NPAT participates in cell cycle regulation and links directly to the control of histone gene expression during the G1/S phase transition. Its presence in the nuclear matrix further highlights its integrative role in gene regulation.
Biological function summary

NPAT plays an important role in the cell cycle's advancement by associating with the MMB (Myb-MuvB) complex. This interaction signifies its importance in DNA replication and cell cycle progression. NPAT activates transcription upon phosphorylation by CDK2/cyclin E influencing S-phase entry. It acts as a transcriptional regulator for histone gene expression necessary for chromatin structure and genome stability.

Pathways

NPAT integrates into the cyclin/CDK regulatory pathway governing cell cycle progression and is related to histone gene clusters regulation. The interaction with CDK2 illustrates its part in the regulation of cell proliferation. NPAT also associates with the E2F cell cycle pathway further influencing gene transcription important for DNA replication.

NPAT relates to cancer and ataxia-telangiectasia. These connections highlight its importance in cell cycle control disruption which can lead to oncogenesis. NPAT's relationship with the ATM protein emerges in the context of ataxia-telangiectasia suggesting a link between DNA damage response defects and neurological disorders. These associations reinforce NPAT’s involvement in maintaining cellular health and genomic integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for progression through the G1 and S phases of the cell cycle and for S phase entry. Activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with MIZF. Also positively regulates the ATM, MIZF and PRKDC promoters. Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters.
See full target information NPAT
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 115:347-356 PubMed38129137

2023

Cancer cell-derived exosomal miR-20a-5p inhibits CD8 T-cell function and confers anti-programmed cell death 1 therapy resistance in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weina Li,Guohui Han,Feng Li,Peng Bu,Yating Hao,Li Huang,Xiangdong Bai

Experimental and therapeutic medicine 25:29 PubMed36561617

2022

Altered genome‑wide hydroxymethylation analysis for neoadjuvant chemoradiotherapy followed by surgery in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xianjing Zhang,Mingzhu Lu,Jing Zhu,Changsong Zhang,Meihua Wang
View all publications
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