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AB308163

Anti-NPEPPS antibody [HL1531]

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Rabbit Recombinant Monoclonal NPEPPS antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Dog, Human, Cat, Rat, Mouse, Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human NPEPPS.

View Alternative Names

PSA, NPEPPS, Puromycin-sensitive aminopeptidase, Cytosol alanyl aminopeptidase, AAP-S

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NPEPPS antibody [HL1531] (AB308163)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NPEPPS antibody [HL1531] (AB308163)

For immunofluorescence analysis, HeLa cells were fixed in ice-cold MeOH for 5 min and permeabilized for detection of NPEPPS protein at cytoplasm using ab308163 at 1/500 dilution (green).

Alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1/1000 (red)

Nuclei stained with Fluoroshield with DAPI (blue)

The observed subcellular location is based on the publication : PMID : 28259985

Immunohistochemistry paraffin embedded sections - Anti-NPEPPS antibody [HL1531] (AB308163)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-NPEPPS antibody [HL1531] (AB308163)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling NPEPPS using ab308163 at 1/100 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6.0 for 15 mins.

Western blot - Anti-NPEPPS antibody [HL1531] (AB308163)
  • WB

Supplier Data

Western blot - Anti-NPEPPS antibody [HL1531] (AB308163)

Separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-NPEPPS antibody [HL1531] (ab308163) at 1/1000 dilution

All lanes:

whole zebrafish lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 103.3 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1531

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human, Dog, Cat, Zebrafish

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NPEPPS. The exact immunogen used to generate this antibody is proprietary information.

P55786

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NPEPPS also known as aminopeptidase puromycin sensitive or APP is a metalloprotease with an approximate molecular mass of 103 kDa. It functions by catalyzing the cleavage of N-terminal amino acids from peptides which is important for many cellular processes. NPEPPS localizes to both the cytoplasm and nucleus within cells and it shows widespread expression across various tissues especially in the brain heart and liver. Its activity plays an important role in the breakdown and recycling of intracellular proteins.
Biological function summary

NPEPPS influences protein turnover by participating in peptide degradation and processing. The enzyme associates with complexes involved in antigen processing impacting the production of antigenic peptides presented by MHC class I molecules. By regulating peptide availability NPEPPS facilitates immune responses and maintains cellular protein homeostasis. This regulatory effect makes it important for several physiological and cellular functions emphasizing its involvement beyond simple peptide cleavage.

Pathways

The NPEPPS enzyme influences the ubiquitin-proteasome system an essential cellular mechanism for protein degradation. This pathway requires NPEPPS for the final trimming of peptides before they are presented by the immune system. The enzyme's activity also connects to the mTOR signaling pathway which controls cell growth and metabolism. Proteins such as ubiquitin and components of the proteasome interact with NPEPPS linking its mechanical function to larger cellular and metabolic pathways.

NPEPPS has associations with neurodegenerative diseases like Alzheimer's disease and Huntington's disease. Altered enzyme function can affect peptide accumulation potentially contributing to the pathological protein aggregates observed in these diseases. Investigations also show a connection to proteins such as amyloid precursor protein implicating NPEPPS in pathways leading to amyloid plaque formation in Alzheimer's. These interactions highlight the potential impact of NPEPPS in disease mechanisms and as a target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain.
See full target information NPEPPS
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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