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AB211422

Anti-NPHP1 antibody

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(1 Publication)

Rabbit Polyclonal NPHP1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NPHP1 aa 1-150.

View Alternative Names

NPH1, NPHP1, Nephrocystin-1, Juvenile nephronophthisis 1 protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHP1 antibody (AB211422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHP1 antibody (AB211422)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling NPHP1 with ab211422 at 10 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHP1 antibody (AB211422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHP1 antibody (AB211422)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling NPHP1 with ab211422 at 10 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NPHP1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O15259

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Precipitation Caprylic Acid and Ammonium Sulphate
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NPHP1 also known as Nephrocystin-1 plays an important role in maintaining the function and structure of primary cilia. This protein has a molecular mass of approximately 79 kDa. NPHP1 is primarily expressed in the renal epithelial cells but it also shows expression in other tissues such as the eye and brain. The protein localizes to the cell's centrosome which acts as an important hub for microtubule organization and organelle trafficking.
Biological function summary

Nephrocystin-1 forms part of a ciliary protein complex involved in the signal transduction pathways that regulate cell proliferation and differentiation. Together with other proteins like INVS and NPHP4 Nephrocystin-1 contributes to the maintenance of ciliary function essential for sensing fluid flow in kidney ducts and other mechanosensory processes. The protein complex influences the positioning and anchoring of ciliary basal bodies contributing to ciliogenesis.

Pathways

Nephrocystin-1 participates in essential cellular signaling pathways particularly the Wnt signaling pathway and Hedgehog signaling. Through these pathways Nephrocystin-1 interacts with proteins such as Dishevelled and Gli impacting cellular processes like development migration and polarity. Disruptions in these pathways can lead to defects in tissue homeostasis underlining NPHP1's role in proper cellular function and response to external signals.

Mutations or malfunctions in Nephrocystin-1 can lead to nephronophthisis a form of hereditary kidney disease and Senior-Loken syndrome a disorder combining nephronophthisis with retinal degeneration. Nephrocystin-1 is closely linked with other ciliary proteins such as NEK8 and CEP290 which also participate in the pathological developments of these disorders. Effective functioning of NPHP1 and these associated proteins is essential for preventing the ciliary abnormalities observed in such ciliopathies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Together with BCAR1 it may play a role in the control of epithelial cell polarity (By similarity). Involved in the organization of apical junctions in kidney cells together with NPHP4 and RPGRIP1L/NPHP8 (By similarity). Does not seem to be strictly required for ciliogenesis (By similarity). Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling (By similarity). May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly. Required for normal retina development (By similarity). In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19. Involved in spermatogenesis (By similarity).
See full target information NPHP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 9:498 PubMed33850895

2021

Circular RNA hsa-circ-000881 suppresses the progression of lung adenocarcinoma via a miR-665/PRICKLE2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chu Huang,Weiming Yue,Lin Li,Shuhai Li,Cun Gao,Libo Si,Lei Qi,Chuanle Cheng,Ming Lu,Guanqing Chen,Jingjing Cui,Renchang Zhao,Yongmeng Li,Hui Tian
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Product promise

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