AB177242

Anti-NPHS2 antibody

Name: Anti-NPHS2 antibody (ab177242) | Abcam
Brand: Abcam Limited
SKU: AB177242
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Goat Polyclonal NPHS2 antibody. Suitable for WB and reacts with Mouse, Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Podocin aa 350 to C-terminus.

View Alternative Names

Podocin, NPHS2

2 Images

Supplier Data

Western blot - Anti-NPHS2 antibody (AB177242)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-NPHS2 antibody (ab177242) at 0.3 µg/mL

Lane 1:

Human kidney lysate (in RIPA buffer) at 35 µg

Lane 2:

Mouse kidney lysate (in RIPA buffer) at 35 µg

Predicted band size: 42 kDa

Observed band size: 45 kDa

true

Supplier Data

Western blot - Anti-NPHS2 antibody (AB177242)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-NPHS2 antibody (ab177242) at 1 µg/mL

All lanes:

Pig kidney lysate (in RIPA buffer) at 35 µg

Predicted band size: 42 kDa

Observed band size: 45 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Pig

Applications

applications

Immunogen

Synthetic Peptide within Human Podocin aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NP85

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab177242 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NPHS2 gene encodes the protein known as podocin which plays an important role in the renal system. Podocin is a membrane protein with a molecular mass of approximately 42 kDa. It is largely expressed in the kidney specifically in the podocyte foot processes of the glomerulus. The protein is responsible for the proper functioning of the slit diaphragm a critical component in the kidney's filtration barrier. NPHS2 by maintaining the structural integrity of these cells prevents proteins from spilling into the urine.

Biological function summary

The function of the NPHS2 gene product is significant in the maintenance of the glomerular filtration barrier. Podocin is a part of the slit diaphragm complex interacting closely with other proteins like nephrin. This interaction is required for the formation and stabilization of the slit diaphragm which filters blood plasma in the glomerulus. The precision in its location and function ensures the selective permeability of the filtration barrier.

Pathways

NPHS2 is heavily involved in pathways that regulate kidney function and filtration processes. It is an important component of the slit diaphragm complex pathway. Podocin’s interaction with proteins like nephrin and CD2AP integrates it into signaling pathways important for cytoskeletal dynamics and cell adhesion in podocytes. These interactions play an essential role in facilitating the response of podocytes to mechanical stress and in maintaining the structure of the glomerular filtration barrier.

NPHS2 mutations link directly to steroid-resistant nephrotic syndrome (SRNS). This condition leads to proteinuria and progressive kidney failure. Podocin dysfunction disrupts the integrity of the renal filtration barrier causing this disease. The protein nephrin is another component in SRNS pathophysiology as both are fundamental to maintaining the structure and function of the slit diaphragm. Understanding the relationship between NPHS2 and these proteins can aid in developing targeted therapies for SRNS and related nephropathies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

See full target information Podocin

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 62: PubMed36734282

2023

FUT2 promotes the tumorigenicity and metastasis of colorectal cancer cells via the Wnt/β‑catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Liu,Jingyu Liu,Mengyang Ding,Yijing Liu,Yue Zhang,Xiaoming Chen,Zhenxu Zhou

Journal of the American Society of Nephrology : JASN 34:88-109 PubMed36167728

2022

Kidney Organoids Generated Using an Allelic Series of NPHS2 Point Variants Reveal Distinct Intracellular Podocin Mistrafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Aude Dorison,Irene Ghobrial,Alison Graham,Thanushi Peiris,Thomas A Forbes,Michael See,Mithun Das,Moin A Saleem,Catherine Quinlan,Kynan T Lawlor,Mirana Ramialison,Sara E Howden,Melissa H Little

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NPHS2 antibody

Western blot - Anti-NPHS2 antibody (AB177242)

Western blot - Anti-NPHS2 antibody (AB177242)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise