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AB50339

Anti-NPHS2 antibody

5

(10 Reviews)

|

(113 Publications)

Anti-NPHS2 antibody (ab50339) is a rabbit polyclonal antibody detecting NPHS2 in Western Blot, IHC-P. Suitable for Mouse, Rat.

- Over 90 publications
- Trusted since 2007

View Alternative Names

Podocin, NPHS2

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NPHS2 with ab50339 at 0.2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)

Immunohistochemical analysis of formalin-fixed, paraffn-embedded mouse kidney tissue using ab50339 at 0.2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)

Immunohistochemical analysis of formalin-fixed, paraffn-embedded rat kidney tissue using ab50339 at 0.2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NPHS2 with ab50339 at 0.2 μg/ml.

Western blot - Anti-NPHS2 antibody (AB50339)
  • WB

Supplier Data

Western blot - Anti-NPHS2 antibody (AB50339)

All lanes:

Western blot - Anti-NPHS2 antibody (ab50339) at 1 µg/mL

Lane 1:

Rat glomeruli tissue extract

Lane 2:

Rat glomeruli tissue extract with Immunizing peptide

Secondary

All lanes:

Anti_rabbit IgG-peroxidase

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Podocin aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9NP85

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p>Additional weak bands may be detected when immunoblotting various extract preparations. Detection of podocin (doublet) by immunoblotting is specifically inhibited with the immunizing peptide. The upper band may represent a post-translational modified variant.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.2 µg/mL", "IHCP-species-notes": "<p></p>" } } }

Product details

What is this antibody validated in?
Anti-NPHS2 antibody (ab50339) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse, Rat samples.

What is the molecular weight of NPHS2?
Anti-NPHS2 (ab50339) specifically detects a band for NPHS2 (UniProt: Q9NP85) at a molecular weight of 42kDa.

Trusted by the scientific community
Anti-NPHS2 (ab50339) was first used in a scientific publication in 2007 and has been cited over 90 times in peer-reviewed journals.

Reviewed by scientists
Anti-NPHS2 (ab50339) has over 10 independent reviews from customers.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab50339 is affinity-purified using the immunizing peptide immobilized on agarose.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NPHS2 gene encodes the protein known as podocin which plays an important role in the renal system. Podocin is a membrane protein with a molecular mass of approximately 42 kDa. It is largely expressed in the kidney specifically in the podocyte foot processes of the glomerulus. The protein is responsible for the proper functioning of the slit diaphragm a critical component in the kidney's filtration barrier. NPHS2 by maintaining the structural integrity of these cells prevents proteins from spilling into the urine.
Biological function summary

The function of the NPHS2 gene product is significant in the maintenance of the glomerular filtration barrier. Podocin is a part of the slit diaphragm complex interacting closely with other proteins like nephrin. This interaction is required for the formation and stabilization of the slit diaphragm which filters blood plasma in the glomerulus. The precision in its location and function ensures the selective permeability of the filtration barrier.

Pathways

NPHS2 is heavily involved in pathways that regulate kidney function and filtration processes. It is an important component of the slit diaphragm complex pathway. Podocin’s interaction with proteins like nephrin and CD2AP integrates it into signaling pathways important for cytoskeletal dynamics and cell adhesion in podocytes. These interactions play an essential role in facilitating the response of podocytes to mechanical stress and in maintaining the structure of the glomerular filtration barrier.

NPHS2 mutations link directly to steroid-resistant nephrotic syndrome (SRNS). This condition leads to proteinuria and progressive kidney failure. Podocin dysfunction disrupts the integrity of the renal filtration barrier causing this disease. The protein nephrin is another component in SRNS pathophysiology as both are fundamental to maintaining the structure and function of the slit diaphragm. Understanding the relationship between NPHS2 and these proteins can aid in developing targeted therapies for SRNS and related nephropathies.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
See full target information Podocin

Publications (113)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes research 2025:2379872 PubMed41069927

2025

Transcriptomics and Proteomics Reveal That TLPW Acupuncture Ameliorates Proteinuria in Diabetic Kidney Disease Model Rats by Suppressing Epithelial-to-Mesenchymal Transition via the DPP4/SDF-1/TGF-/Smad Signalling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Ji,Jiya Sun,Zihao Zhuang,Yunming Xiao,Shipian Li,Zhilong Zhang,Xu Wang,Xinju Li

Cell death & disease 16:185 PubMed40108127

2025

NAT10 mediates TLR2 to promote podocyte senescence in adriamycin-induced nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyang Hu,Linxiao Lv,Yuqi Lei,Min Chen,Sijie Zhou,Zhangsuo Liu

Open life sciences 19:20220940 PubMed39588120

2024

Renalase protects against podocyte injury by inhibiting oxidative stress and apoptosis in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yiru Wu,Yiduo Feng,Yue Yu,Yu Bai,Zongli Diao,Wenhu Liu

Matrix biology plus 24:100164 PubMed39582511

2024

A human stem cell-derived model reveals pathologic extracellular matrix remodeling in diabetic podocyte injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmin Roye,Carmen Miller,Titilola D Kalejaiye,Samira Musah

Cell death & disease 15:791 PubMed39500879

2024

OTUB1 regulation of ferroptosis and the protective role of ferrostatin-1 in lupus nephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Liu,Yu-Hui Gan,Wei-Jing Yong,Hong-de Xu,Yong-Chun Li,Hui-Miao Hu,Zhan-Zheng Zhao,Yuan-Yuan Qi

Bioengineering (Basel, Switzerland) 11: PubMed39451413

2024

Mechanosensitive Differentiation of Human iPS Cell-Derived Podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yize Zhang,Samira Musah

Science advances 10:eadn2689 PubMed38838141

2024

An ultrathin membrane mediates tissue-specific morphogenesis and barrier function in a human kidney chip.

Applications

Unspecified application

Species

Unspecified reactive species

Xingrui Mou,Jessica Shah,Yasmin Roye,Carolyn Du,Samira Musah

Cell stem cell 31:921-939.e17 PubMed38692273

2024

Long-term expandable mouse and human-induced nephron progenitor cells enable kidney organoid maturation and modeling of plasticity and disease.

Applications

Unspecified application

Species

Unspecified reactive species

Biao Huang,Zipeng Zeng,Sunghyun Kim,Connor C Fausto,Kari Koppitch,Hui Li,Zexu Li,Xi Chen,Jinjin Guo,Chennan C Zhang,Tianyi Ma,Pedro Medina,Megan E Schreiber,Mateo W Xia,Ariel C Vonk,Tianyuan Xiang,Tadrushi Patel,Yidan Li,Riana K Parvez,Balint Der,Jyun Hao Chen,Zhenqing Liu,Matthew E Thornton,Brendan H Grubbs,Yarui Diao,Yali Dou,Ksenia Gnedeva,Qilong Ying,Nuria M Pastor-Soler,Teng Fei,Kenneth R Hallows,Nils O Lindström,Andrew P McMahon,Zhongwei Li

Renal failure 46:2318413 PubMed38369750

2024

The potential role of differentially expressed tRNA-derived fragments in high glucose-induced podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenxing Zhang,Yunyang Qiao,Jialing Ji,Chan Huang,Huimin Shi,Weihua Gan,Aiqing Zhang

Acta physiologica (Oxford, England) 240:e14108 PubMed38314444

2024

SGLT2 inhibition promotes glomerular repopulation by cells of renin lineage in experimental kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Loïs A K van der Pluijm,Angela Koudijs,Wendy Stam,Joris J T H Roelofs,A H Jan Danser,Joris I Rotmans,Kenneth W Gross,Michael P Pieper,Anton Jan van Zonneveld,Roel Bijkerk
View all publications

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