Anti-NPHS2 antibody
5
(10 Reviews)
|
(113 Publications)
Anti-NPHS2 antibody (ab50339) is a rabbit polyclonal antibody detecting NPHS2 in Western Blot, IHC-P. Suitable for Mouse, Rat.
- Over 90 publications
- Trusted since 2007
View Alternative Names
Podocin, NPHS2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NPHS2 with ab50339 at 0.2 μg/ml.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)
Immunohistochemical analysis of formalin-fixed, paraffn-embedded mouse kidney tissue using ab50339 at 0.2 μg/ml.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)
Immunohistochemical analysis of formalin-fixed, paraffn-embedded rat kidney tissue using ab50339 at 0.2 μg/ml.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody (AB50339)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NPHS2 with ab50339 at 0.2 μg/ml.
- WB
Supplier Data
Western blot - Anti-NPHS2 antibody (AB50339)
All lanes:
Western blot - Anti-NPHS2 antibody (ab50339) at 1 µg/mL
Lane 1:
Rat glomeruli tissue extract
Lane 2:
Rat glomeruli tissue extract with Immunizing peptide
Secondary
All lanes:
Anti_rabbit IgG-peroxidase
Predicted band size: 42 kDa
false
Reactivity data
Product details
Anti-NPHS2 antibody (ab50339) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse, Rat samples.
What is the molecular weight of NPHS2?
Anti-NPHS2 (ab50339) specifically detects a band for NPHS2 (UniProt: Q9NP85) at a molecular weight of 42kDa.
Trusted by the scientific community
Anti-NPHS2 (ab50339) was first used in a scientific publication in 2007 and has been cited over 90 times in peer-reviewed journals.
Reviewed by scientists
Anti-NPHS2 (ab50339) has over 10 independent reviews from customers.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The function of the NPHS2 gene product is significant in the maintenance of the glomerular filtration barrier. Podocin is a part of the slit diaphragm complex interacting closely with other proteins like nephrin. This interaction is required for the formation and stabilization of the slit diaphragm which filters blood plasma in the glomerulus. The precision in its location and function ensures the selective permeability of the filtration barrier.
Pathways
NPHS2 is heavily involved in pathways that regulate kidney function and filtration processes. It is an important component of the slit diaphragm complex pathway. Podocin’s interaction with proteins like nephrin and CD2AP integrates it into signaling pathways important for cytoskeletal dynamics and cell adhesion in podocytes. These interactions play an essential role in facilitating the response of podocytes to mechanical stress and in maintaining the structure of the glomerular filtration barrier.
Product protocols
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Target data
Publications (113)
Recent publications for all applications. Explore the full list and refine your search
Journal of diabetes research 2025:2379872 PubMed41069927
2025
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Cell death & disease 16:185 PubMed40108127
2025
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Open life sciences 19:20220940 PubMed39588120
2024
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Matrix biology plus 24:100164 PubMed39582511
2024
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Cell death & disease 15:791 PubMed39500879
2024
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Bioengineering (Basel, Switzerland) 11: PubMed39451413
2024
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Science advances 10:eadn2689 PubMed38838141
2024
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Cell stem cell 31:921-939.e17 PubMed38692273
2024
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Renal failure 46:2318413 PubMed38369750
2024
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Acta physiologica (Oxford, England) 240:e14108 PubMed38314444
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com