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Rabbit Recombinant Monoclonal NPHS2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 19 publications.

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Images

Western blot - Anti-NPHS2 antibody [EPR13820] (AB181143), expandable thumbnail
  • Western blot - Anti-NPHS2 antibody [EPR13820] (AB181143), expandable thumbnail
  • Western blot - Anti-NPHS2 antibody [EPR13820] (AB181143), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (AB181143), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (AB181143), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Not recommended
Mouse
Expected
Tested
Not recommended
Rat
Expected
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

For unpurified use at 1/10000 - 1/50000.

Species
Human
Dilution info
1/2000
Notes

For unpurified use at 1/10000 - 1/50000.

Species
Rat
Dilution info
1/2000
Notes

For unpurified use at 1/10000 - 1/50000.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

Alternative names

Podocin, NPHS2

Recommended products

Rabbit Recombinant Monoclonal NPHS2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 19 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13820
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The NPHS2 gene encodes the protein known as podocin which plays an important role in the renal system. Podocin is a membrane protein with a molecular mass of approximately 42 kDa. It is largely expressed in the kidney specifically in the podocyte foot processes of the glomerulus. The protein is responsible for the proper functioning of the slit diaphragm a critical component in the kidney's filtration barrier. NPHS2 by maintaining the structural integrity of these cells prevents proteins from spilling into the urine.

Biological function summary

The function of the NPHS2 gene product is significant in the maintenance of the glomerular filtration barrier. Podocin is a part of the slit diaphragm complex interacting closely with other proteins like nephrin. This interaction is required for the formation and stabilization of the slit diaphragm which filters blood plasma in the glomerulus. The precision in its location and function ensures the selective permeability of the filtration barrier.

Pathways

NPHS2 is heavily involved in pathways that regulate kidney function and filtration processes. It is an important component of the slit diaphragm complex pathway. Podocin’s interaction with proteins like nephrin and CD2AP integrates it into signaling pathways important for cytoskeletal dynamics and cell adhesion in podocytes. These interactions play an essential role in facilitating the response of podocytes to mechanical stress and in maintaining the structure of the glomerular filtration barrier.

Associated diseases and disorders

NPHS2 mutations link directly to steroid-resistant nephrotic syndrome (SRNS). This condition leads to proteinuria and progressive kidney failure. Podocin dysfunction disrupts the integrity of the renal filtration barrier causing this disease. The protein nephrin is another component in SRNS pathophysiology as both are fundamental to maintaining the structure and function of the slit diaphragm. Understanding the relationship between NPHS2 and these proteins can aid in developing targeted therapies for SRNS and related nephropathies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143), expandable thumbnail

    Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143)

    All lanes: Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/10000 dilution

    All lanes: Human fetal kidney at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa

  • Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143), expandable thumbnail

    Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143)

    All lanes: Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/50000 dilution

    All lanes: Human fetal kidney tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

  • Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143), expandable thumbnail

    Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143)

    All lanes: Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/2000 dilution

    Lane 1: Mouse kidney tissue lysate at 20 µg

    Lane 2: Rat kidney tissue lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143)

    ab181143 staining NPHS2 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143)

    ab181143 staining NPHS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (ab181143)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NPHS2 with unpurified ab181143 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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