AB181143

Anti-NPHS2 antibody [EPR13820]

RabMAb
Recombinant
20ul selling size
What is this?

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(37 Publications)

Anti-NPHS2 antibody [EPR13820] (ab181143) is a rabbit monoclonal antibody detecting NPHS2 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

Podocin, NPHS2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (AB181143)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (AB181143)

ab181143 staining NPHS2 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (AB181143)

ab181143 staining NPHS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (AB181143)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NPHS2 with unpurified ab181143 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-NPHS2 antibody [EPR13820] (AB181143)

All lanes:

Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/50000 dilution

All lanes:

Human fetal kidney tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Lab

Western blot - Anti-NPHS2 antibody [EPR13820] (AB181143)

All lanes:

Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/10000 dilution

All lanes:

Human fetal kidney at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 42 kDa

false

Lab

Western blot - Anti-NPHS2 antibody [EPR13820] (AB181143)

All lanes:

Western blot - Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/2000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Carrier free

Anti-NPHS2 antibody [EPR13820] - BSA and Azide free
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-NPHS2 antibody [EPR13820]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-NPHS2 antibody [EPR13820]
578 PE

PE Anti-NPHS2 antibody [EPR13820]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-NPHS2 antibody [EPR13820]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-NPHS2 antibody [EPR13820]
660 APC

APC Anti-NPHS2 antibody [EPR13820]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-NPHS2 antibody [EPR13820]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13820

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/10000 - 1/50000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/10000 - 1/50000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/10000 - 1/50000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-NPHS2 antibody [EPR13820] (ab181143) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of NPHS2?
Anti-NPHS2 [EPR13820] (ab181143) specifically detects a band for NPHS2 (UniProt: Q9NP85) at a molecular weight of 42kDa.

Trusted by the scientific community
Anti-NPHS2 [EPR13820] (ab181143) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR13820] also available for your convenience: ab181143, Carrier free - ab240244, APC - ab319213, PE - ab319361, Alexa Fluor® 488 - ab319495, Alexa Fluor® 647 - ab319608, Alexa Fluor® 594 - ab319712, Alexa Fluor® 555 - ab319854, Alexa Fluor® 750 - ab321247

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NPHS2 gene encodes the protein known as podocin which plays an important role in the renal system. Podocin is a membrane protein with a molecular mass of approximately 42 kDa. It is largely expressed in the kidney specifically in the podocyte foot processes of the glomerulus. The protein is responsible for the proper functioning of the slit diaphragm a critical component in the kidney's filtration barrier. NPHS2 by maintaining the structural integrity of these cells prevents proteins from spilling into the urine.

Biological function summary

The function of the NPHS2 gene product is significant in the maintenance of the glomerular filtration barrier. Podocin is a part of the slit diaphragm complex interacting closely with other proteins like nephrin. This interaction is required for the formation and stabilization of the slit diaphragm which filters blood plasma in the glomerulus. The precision in its location and function ensures the selective permeability of the filtration barrier.

Pathways

NPHS2 is heavily involved in pathways that regulate kidney function and filtration processes. It is an important component of the slit diaphragm complex pathway. Podocin's interaction with proteins like nephrin and CD2AP integrates it into signaling pathways important for cytoskeletal dynamics and cell adhesion in podocytes. These interactions play an essential role in facilitating the response of podocytes to mechanical stress and in maintaining the structure of the glomerular filtration barrier.

NPHS2 mutations link directly to steroid-resistant nephrotic syndrome (SRNS). This condition leads to proteinuria and progressive kidney failure. Podocin dysfunction disrupts the integrity of the renal filtration barrier causing this disease. The protein nephrin is another component in SRNS pathophysiology as both are fundamental to maintaining the structure and function of the slit diaphragm. Understanding the relationship between NPHS2 and these proteins can aid in developing targeted therapies for SRNS and related nephropathies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

See full target information Podocin

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:398 PubMed40448086

2025

TRPC6-targeted dexamethasone nanobubbles with ultrasound-guided theranostics for adriamycin-induced nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Wu,Yang Liu,Ziqi Fu,Honglei Guo,Kang Liu,Jiafa Ren,Zhimin Huang,Fang Yang,Huijuan Mao

Renal failure 47:2490200 PubMed40260530

2025

Circ-0069561 as a novel diagnostic biomarker for progression of diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoyi Chen,Xinran Liu,Sai Zhu,Yukai Wang,Yu Ma,Ziyun Hu,Yonggui Wu,Ling Jiang

Molecular medicine (Cambridge, Mass.) 31:88 PubMed40050708

2025

Empagliflozin ameliorates renal and metabolic derangements in obese type 2 diabetic mice by blocking advanced glycation end product-receptor axis.

Applications

Unspecified application

Species

Unspecified reactive species

Takanori Matsui,Ami Sotokawauchi,Yuri Nishino,Yoshinori Koga,Sho-Ichi Yamagishi

Renal failure 47:2459896 PubMed39972601

2025

Modified Huangqi Chifeng decoction alleviates podocyte injury on rat with experimental membranous nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Meiying Chang,Xiujie Shi,Bin Yang,Peng Li,Yifan Zhang,Qi Zhang,Yu Zhang

International journal of endocrinology 2025:4473803 PubMed39882551

2025

Puerarin Attenuates Podocyte Damage in Mice With Diabetic Kidney Disease by Modulating the AMPK/Nrf2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Song Xue,Wei Fan,Qingping Li,Hong Huang,Yibo Tang,Min Wu

World journal of diabetes 15:1916-1931 PubMed39280180

2024

Corilagin alleviates podocyte injury in diabetic nephropathy by regulating autophagy the SIRT1-AMPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Lou,Yu-Ting Luan,Wen-Qing Rong,Yun Gai

International journal of biological sciences 20:3317-3333 PubMed38993555

2024

Ursolic Acid Alleviates Mitotic Catastrophe in Podocyte by Inhibiting Autophagic P62 Accumulation in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Mei,Tienan Jing,Haojun Liu,Yue Liu,Xinwang Zhu,Jiao Wang,Li Xu

Nephrology (Carlton, Vic.) 29:482-494 PubMed38837564

2024

Rehmapicrogenin attenuates lipopolysaccharide-induced podocyte injury and kidney dysfunctions by regulating nuclear factor E2-related factor 2/antioxidant response element signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Ma,Guandong Li,Yufeng Shi,Zhitao Shang

Toxicology research 13:tfae079 PubMed38828128

2024

STAT3-induced upregulation of lncRNA TTN-AS1 aggravates podocyte injury in diabetic nephropathy by promoting oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzhe Wang,Yongxia Li,Fan Zhu,Yunfang Huang

Acta biochimica et biophysica Sinica 56:1311-1322 PubMed38808395

2024

Aminophylline suppresses chronic renal failure progression by activating SIRT1/AMPK/mTOR-dependent autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Liao,Jieyi Lu,Zhifeng Huang,Jinai Lin,Miao Zhang,Huanru Chen,Xiaoqing Lin,Xia Gao,Sitang Gong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NPHS2 antibody [EPR13820]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (AB181143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (AB181143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR13820] (AB181143)

Western blot - Anti-NPHS2 antibody [EPR13820] (AB181143)

Western blot - Anti-NPHS2 antibody [EPR13820] (AB181143)

Western blot - Anti-NPHS2 antibody [EPR13820] (AB181143)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (37)

Product promise