Anti-NPHS2 antibody [EPR22203-219] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPHS2 antibody [EPR22203-219] - BSA and Azide free (AB239758)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling NPHS2 with ab229037 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human glomeruli and weak staining on renal tubules (PMID : 21499232; PMID : 24559085; PMID : 11786407) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229037).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-NPHS2 antibody [EPR22203-219] - BSA and Azide free (AB239758)

This data was developed using ab229037, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining UMOD with ab255825 at 1/1000 (0.5 μg/ml), ab229037 anti-NPHS2, and ab326049 anti-Renin-1+ Renin-2.

Panel A : anti-UMOD (green; Opal™520), anti-NPHS2 (magenta; Opal™690), anti-Renin-1+Renin-2 (gray; Opal™570) on mouse kidney.

Panel B : anti-UMOD staining the thick ascending limb of the loop of Henle (TALH) in mouse kidney.

Panel C : anti-NPHS2 staining glomerular podocyte in mouse kidney.

Panel D : anti-Renin-1+Renin-2 staining juxtaglomerular cells in mouse kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab255825, ab229037 and ab326049 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-NPHS2 antibody [EPR22203-219] - BSA and Azide free (AB239758)

This data was developed using ab229037, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat kidney tissue staining UMOD with ab255825 at 1/1000 (0.5 μg/ml), ab229037 anti-NPHS2, and ab326049 anti-Renin-1+ Renin-2.

Panel A : anti-UMOD (green; Opal™520), anti-NPHS2 (magenta; Opal™690), anti-Renin-1+Renin-2 (gray; Opal™570) on rat kidney.

Panel B : anti-UMOD staining the thick ascending limb of the loop of Henle (TALH) in rat kidney.

Panel C : anti-NPHS2 staining glomerular podocyte in rat kidney.

Panel D : anti-Renin-1+Renin-2 staining juxtaglomerular cells in rat kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab255825, ab229037 and ab326049 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.