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AB176383

Anti-NPL4 antibody

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(1 Publication)

Rabbit Polyclonal NPL4 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NPLOC4 aa 500 to C-terminus.

View Alternative Names

KIAA1499, NPL4, NPLOC4, Nuclear protein localization protein 4 homolog, Protein NPL4

1 Images
Immunoprecipitation - Anti-NPL4 antibody (AB176383)
  • IP

Unknown

Immunoprecipitation - Anti-NPL4 antibody (AB176383)

Detection of NPL4 in immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176383 at 6 μg/mg lysate for IP. Lane 2 represents control IgG IP. An anti-NPL4 antibody was used at 0.1 μg/ml for subsequent western blot detection.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-NPL4 antibody (ab176383)

Predicted band size: 68 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human NPLOC4 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TAT6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein NPL4 known also as Nuclear Protein Localization 4 is involved in key cellular processes as a cofactor in the p97 ATPase complex. It has a molecular mass of approximately 68 kDa. NPL4 is expressed in different tissues with increased levels observed in dividing cells. Mechanically NPL4 aids in protein degradation by advancing the extraction of ubiquitinated substrates from the ER-associated degradation (ERAD) pathway.
Biological function summary

NPL4 participates in the unfoldase activity required for the ERAD process. It functions within a complex that includes p97/VCP and other cofactors like UFD1. This complex targets misfolded proteins for degradation in the proteasome maintaining cellular proteostasis. In a broader scope NPL4 ensures efficient cellular response to protein stress which is necessary for normal cell cycle progression and mitosis.

Pathways

NPL4 integrates into essential proteostasis pathways interplaying with ERAD and the ubiquitin-proteasome system. It interacts with key proteins like UFD1 and p97/VCP within these pathways. The NPL4-p97-VCP complex facilitates protein dislocation during ER stress therefore influencing the unfolded protein response (UPR) a critical cellular adaptation mechanism.

Malfunction of NPL4 has links to neurodegenerative diseases and certain cancers. Impairments in NPL4 function and its complex with p97/VCP associate with the accumulation of ubiquitinated proteins which contributes to pathological states such as amyotrophic lateral sclerosis (ALS) and various tumor progressions. Mutations within proteins of this complex also play a role in those conditions underpinning the critical importance of NPL4 in cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI (PubMed : 26471729).
See full target information NPLOC4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 10:295 PubMed38909015

2024

USP14 deficiency inhibits neointima formation following vascular injury via degradation of Skp2 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Xia,Xiaolin Liu,Qiong Xu,Jielei Gu,Sisi Ling,Yajing Liu,Rongxue Li,Min Zou,Siqin Jiang,Zhiwei Gao,Canshan Chen,Shiming Liu,Ningning Liu
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Product promise

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