AB224435

Anti-NPL4 antibody

Name: Anti-NPL4 antibody (ab224435) | Abcam
Brand: Abcam Limited
SKU: AB224435
Price: 645 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal NPL4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NPLOC4 aa 350-550.

View Alternative Names

KIAA1499, NPL4, NPLOC4, Nuclear protein localization protein 4 homolog, Protein NPL4

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPL4 antibody (AB224435)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPL4 antibody (AB224435)

Paraffin-embedded human skeletal muscle tissue stained for NPL4 using ab224435 at 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NPL4 antibody (AB224435)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NPL4 antibody (AB224435)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for NPL4 (green) using ab224435 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPL4 antibody (AB224435)

Paraffin-embedded human testis tissue stained for NPL4 using ab224435 at 1/20 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-NPL4 antibody (AB224435)

All lanes:

Western blot - Anti-NPL4 antibody (ab224435) at 1/100 dilution

All lanes:

A549 (human lung carcinoma cell line) cell lysate

Predicted band size: 68 kDa

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Supplier Data

Western blot - Anti-NPL4 antibody (AB224435)

All lanes:

Western blot - Anti-NPL4 antibody (ab224435) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 68 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NPLOC4 aa 350-550. The exact immunogen used to generate this antibody is proprietary information.

Q8TAT6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein NPL4 known also as Nuclear Protein Localization 4 is involved in key cellular processes as a cofactor in the p97 ATPase complex. It has a molecular mass of approximately 68 kDa. NPL4 is expressed in different tissues with increased levels observed in dividing cells. Mechanically NPL4 aids in protein degradation by advancing the extraction of ubiquitinated substrates from the ER-associated degradation (ERAD) pathway.

Biological function summary

NPL4 participates in the unfoldase activity required for the ERAD process. It functions within a complex that includes p97/VCP and other cofactors like UFD1. This complex targets misfolded proteins for degradation in the proteasome maintaining cellular proteostasis. In a broader scope NPL4 ensures efficient cellular response to protein stress which is necessary for normal cell cycle progression and mitosis.

Pathways

NPL4 integrates into essential proteostasis pathways interplaying with ERAD and the ubiquitin-proteasome system. It interacts with key proteins like UFD1 and p97/VCP within these pathways. The NPL4-p97-VCP complex facilitates protein dislocation during ER stress therefore influencing the unfolded protein response (UPR) a critical cellular adaptation mechanism.

Malfunction of NPL4 has links to neurodegenerative diseases and certain cancers. Impairments in NPL4 function and its complex with p97/VCP associate with the accumulation of ubiquitinated proteins which contributes to pathological states such as amyotrophic lateral sclerosis (ALS) and various tumor progressions. Mutations within proteins of this complex also play a role in those conditions underpinning the critical importance of NPL4 in cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI (PubMed : 26471729).

See full target information NPLOC4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:8324 PubMed38594513

2024

A study on the significance of serine hydroxymethyl transferase expression and its role in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Wei Su,Xian Chen,Guang Wang,Pei Li,Tong-Xin Yang,Ke-Wei Fang,Jing Wu,Jiong-Ming Li

Scientific reports 13:20430 PubMed37993584

2023

NPLOC4 is a potential target and a poor prognostic signature in lung squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Naixue Wang,Dantong Zhu,Yao Liu,Jingran Wu,Meiling Wang,Shanxiu Jin,Fangwei Fu,Baolei Li,Hongjuan Ji,Cheng Du,Zhendong Zheng

Bioengineered 13:6579-6589 PubMed35290151

2022

Disulfiram/Copper induces antitumor activity against gastric cancer via the ROS/MAPK and NPL4 pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Liu,Xin Guan,Meiling Wang,Naixue Wang,Yutong Chen,Baolei Li,Zhuxuan Xu,Fangwei Fu,Zhendong Zheng,Cheng Du

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NPL4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPL4 antibody (AB224435)

Immunocytochemistry/ Immunofluorescence - Anti-NPL4 antibody (AB224435)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPL4 antibody (AB224435)

Western blot - Anti-NPL4 antibody (AB224435)

Western blot - Anti-NPL4 antibody (AB224435)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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