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AB243544

Anti-NPM2 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal NPM2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NPM2 aa 100 to C-terminus.

View Alternative Names

Nucleoplasmin-2, NPM2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPM2 antibody (AB243544)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPM2 antibody (AB243544)

Paraffin-embedded human parathyroid gland tissue stained for NPM2 using ab243544 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NPM2 antibody (AB243544)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NPM2 antibody (AB243544)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for NPM2 (green) using ab243544 at 4 μg/ml in ICC/IF. Microtubules are stained iin Red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPM2 antibody (AB243544)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPM2 antibody (AB243544)

Paraffin-embedded human liver tissue stained for NPM2 using ab243544 at 1/50 dilution in immunohistochemical analysis. Low expression as expected.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NPM2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q86SE8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleophosmin/Nucleoplasmin 2 (NPM2) also referred to as Nucleoplasmin-2 is a protein with molecular mass approximately 22 kDa. It belongs to the family of nucleoplasmins which are chromatin remodeling proteins. NPM2 is mainly expressed in oocytes and plays a significant role during early embryonic development. The protein is highly localized in the nuclei of developing oocytes and is known for its capacity to bind and exchange histones which is essential for chromatin structure.
Biological function summary

The function of NPM2 involves regulating chromatin in germ cells and early embryogenesis. It acts as a histone chaperone forming complexes with histones to facilitate chromatin assembly and disassembly. The protein enables the proper packing and unpacking of DNA ensuring genomic stability during cell division. NPM2 does not work alone and it often forms a complex with histone proteins allowing it to modulate chromatin dynamics efficiently.

Pathways

NPM2 participates in key cellular processes related to chromatin remodeling and regulation. It plays an important role in the chromatin assembly pathway working alongside proteins like histone H1 to modulate DNA accessibility. Additionally NPM2 interacts with other histone chaperones affecting the nucleosome assembly pathway. Through these pathways it influences various nuclear processes important for gene expression and maintenance of nuclear architecture.

NPM2 is associated with issues in reproductive health and cancer. Abnormal expression or malfunction of NPM2 can lead to infertility due to defects in oocyte maturation. In cancer biology variations in NPM2 expression levels might correlate with tumor progression where it interacts with several oncogenic proteins. For example changes in the function of NPM2 might influence the activity of histone-modifying enzymes linked to chromatin changes seen in malignant cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Probably involved in sperm DNA decondensation during fertilization.
See full target information NPM2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

World journal of surgical oncology 20:350 PubMed36280841

2022

Loss of NPM2 expression is a potential immunohistochemical marker for malignant peritoneal mesothelioma: a single-center study of 92 cases.

Applications

Unspecified application

Species

Unspecified reactive species

He-Liang Wu,Zhi-Ran Yang,Yan-Dong Su,Ru Ma,Xue-Mei Du,Ying Gao,Yan Li

The journal of gene medicine 22:e3247 PubMed32602607

2020

Long non-coding RNA SNHG17 promotes proliferation, migration and invasion of glioma cells by regulating the miR-23b-3p/ZHX1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bei-Hai Ge,Guo-Cheng Li
View all publications
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