Rabbit Recombinant Monoclonal NPP-7 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-NPP-7 antibody [EPR12329] - BSA and Azide free (AB249792), expandable thumbnail](https://content.abcam.com/products/images/npp-7-antibody-epr12329-bsa-and-azide-free-ab249792--western-blot-img70704.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NPP-7 antibody [EPR12329] - BSA and Azide free (AB249792)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract (PubMed:12671034, PubMed:12885774, PubMed:15205117, PubMed:16255717, PubMed:28292932). Has also phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation (PubMed:12885774, PubMed:16255717). Does not have nucleotide pyrophosphatase activity (PubMed:12885774). May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen (By similarity).
Alternative names
UNQ3077/PRO9912, ENPP7, Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, E-NPP 7, NPP-7, Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-SMase
Recommended products
Rabbit Recombinant Monoclonal NPP-7 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12329
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249792 is the carrier-free version of Anti-NPP-7 antibody [EPR12329] ab174281.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NPP-7 also known as nucleotide pyrophosphatase/phosphodiesterase 7 is an enzyme involved in the hydrolysis of pyrophosphate-containing substrates. It has a molecular mass of approximately 125 kDa. This enzyme exhibits expression in several human tissues prominently in the intestinal tract and kidney. The enzyme's activity facilitates the breakdown of molecules serving roles in both digestion and cellular signaling processes.
- Biological function summary
NPP-7 participates in lipid metabolism and contributes to maintaining homeostasis by degrading extracellular nucleotides. It forms part of larger protein complexes that regulate various cellular activities. Its enzymatic function is important for controlling the concentration of lipid-derived signaling molecules thereby impacting cellular communication and response mechanisms.
- Pathways
NPP-7 integrates into the pathway regulating phospholipid metabolism and nucleotide salvage pathways. It interacts with proteins such as NPP-2 which are involved in similar metabolic processes ensuring balance in lipid signaling and energy storage within cells. Its function in these pathways highlights its role in modulating phospholipid levels and influencing liver function and cellular energy dynamics.
- Associated diseases and disorders
NPP-7 relates to conditions such as metabolic syndrome and chronic kidney disease. Dysregulation or altered expression of NPP-7 can affect lipid metabolism and kidney function contributing to these diseases. This protein interacts with other proteins including ENPP1 which ties into its involvement in disrupting normal cellular metabolism and exacerbating pathological states in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-NPP-7 antibody [EPR12329] - BSA and Azide free (ab249792), expandable thumbnail](https://content.abcam.com/products/images/npp-7-antibody-epr12329-bsa-and-azide-free-ab249792--western-blot-img70704.jpg "Western blot - Anti-NPP-7 antibody [EPR12329] - BSA and Azide free (ab249792)")
Western blot - Anti-NPP-7 antibody [EPR12329] - BSA and Azide free (ab249792)
This data was developed using Anti-NPP-7 antibody [EPR12329] ab174281, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-NPP-7 antibody [EPR12329] (Anti-NPP-7 antibody [EPR12329] ab174281) at 1/10000 dilution
Lane 1: Caco 2 lysate at 10 µg
Lane 2: HT-29 lysate at 10 µg
Lane 3: Human fetal liver lysate at 10 µg
Predicted band size: 51 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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