JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB139188

Anti-NPR-A + NPR-B antibody [EPR5719(2)]

3

(1 Review)

|

(4 Publications)

Rabbit Recombinant Monoclonal NPR-A antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

ANPRA, NPR1, Atrial natriuretic peptide receptor 1, Atrial natriuretic peptide receptor type A, Guanylate cyclase A, ANP-A, ANPR-A, NPR-A, GC-A

2 Images
Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (AB139188)
  • WB

Unknown

Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (AB139188)

All lanes:

Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188) at 1/10000 dilution

Lane 1:

Human heart lysate

Lane 2:

Human kidney lysate

Lane 3:

Mouse brain lysate

Lane 4:

Mouse heart lysate

Lane 5:

Rat brain lysate

Lane 6:

Rat heart lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (AB139188)
  • WB

Lab

Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (AB139188)

Blocking buffer and concentration : 5% NFDM/TBST

Loading Control : Anti-GST antibody, ab111947

All lanes:

Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (ab139188) at 1/1000 dilution

Lane 1:

GST-tagged recombinant human Natriuretic Peptide Receptor A protein (aa 495 to 1061) at 0.01 µg

Lane 2:

GST-tagged recombinant human Natriuretic Peptide Receptor B protein (aa 479 to 1047) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 88 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5719(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen for this product shares 95% homology with NPR-A

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NPR-A and NPR-B are types of natriuretic peptide receptors which operate as transmembrane receptors composed of an extracellular ligand-binding domain a single transmembrane-spanning domain and an intracellular guanylyl cyclase catalytic domain. NPR-A is widely recognized as GC-A while NPR-B is also known as GC-B. These receptors are approximately 120 kDa in size. Both NPR-A and NPR-B are expressed in various tissues including the heart kidneys and vascular smooth muscle cells which suggests a broad physiological role across different body systems.
Biological function summary

These receptors function mainly by binding natriuretic peptides like atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) for NPR-A while C-type natriuretic peptide (CNP) serves as the primary ligand for NPR-B. Their activation leads to the conversion of GTP to cGMP which then functions as a second messenger involved in vasodilation and diuresis. The receptors are essential components of blood pressure regulation and fluid balance through their role in the cGMP complex.

Pathways

For both NPR-A and NPR-B they significantly contribute to the maintenance of cardiovascular homeostasis and kidney function through the natriuretic peptide signaling pathway. They are tightly linked with proteins such as guanylyl cyclase-related proteins and phosphodiesterases which help in the regulation and degradation of cGMP respectively. Furthermore these receptors interact strongly with cyclic nucleotide signal transduction pathways that involve other key proteins including protein kinase G.

NPR-A and NPR-B hold importance due to their role in cardiovascular diseases including hypertension and heart failure. The dysregulation of these receptors alongside ANP and BNP has been associated with these conditions. Moreover they are implicated in skeletal dysplasia disorders due to the involvement of NPR-B in cartilage development where the signaling through NPR-B in chondrocytes plays a significant role. Malfunctions in this receptor system can lead to improper regulation of growth and development linking it with disorders such as achondroplasia alongside CNP.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
See full target information NPR1

Additional targets

NPR2
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 9:981333 PubMed36818914

2023

Angiotensin receptor-neprilysin inhibitor improves coronary collateral perfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Kangbo Li,Victoria Kratzmann,Mengjun Dai,Nora Gatzke,Petra Rocic,Peter Bramlage,Olaf Grisk,Lubomir T Lubomirov,Meike Hoffmeister,Martin A Lauxmann,Oliver Ritter,Eva Buschmann,Michael Bader,Anja Bondke Persson,Ivo Buschmann,Philipp Hillmeister

Endocrinology 163: PubMed35041746

2022

C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Keisho Hirota,Tsuyoshi Hirashima,Kazuki Horikawa,Akihiro Yasoda,Michiyuki Matsuda

Molecular medicine reports 20:5091-5099 PubMed31638216

2019

C‑type natriuretic peptide prevents angiotensin II‑induced atrial connexin 40 and 43 dysregulation by activating AMP‑activated kinase signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Da-Zhi Ding,Ya-Nan Jia,Bo Zhang,Cheng-Ming Guan,Shuai Zhou,Xiang Li,Xun Cui

PeerJ 7:e6942 PubMed31143551

2019

Identification of RyR2-PBmice and the effects of transposon insertional mutagenesis of the RyR2 gene on cardiac function in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Wang,Chao Wang,Bo Wang,Qirui Shen,Leilei Qiu,Shuaijun Zou,Tao Wang,Guoyan Liu,Beilei Wang,Liming Zhang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB179483

Anti-HIF-1 alpha antibody [EPR16897]

RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [EPR16897] (AB179483)

  • 4
  • 9
Primary Antibodies

AB166628

Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (AB166628)

  • 4
  • 5
Primary Antibodies

AB133327

Anti-CDK1 antibody [EPR165]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CDK1 antibody [EPR165] (AB133327)

  • 5
  • 4
Primary Antibodies

AB174854

Anti-WNK1 antibody [EPR2743(3)]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-WNK1 antibody [EPR2743(3)] (AB174854)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com