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AB248869

Anti-NPR-A + NPR-B antibody [EPR5719(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NPR-A antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human, Recombinant fragment - Human samples. Cited in 1 publication.

View Alternative Names

ANPRA, NPR1, Atrial natriuretic peptide receptor 1, Atrial natriuretic peptide receptor type A, Guanylate cyclase A, ANP-A, ANPR-A, NPR-A, GC-A

2 Images
Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] - BSA and Azide free (AB248869)
  • WB

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Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] - BSA and Azide free (AB248869)

All lanes:

Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (<a href='/en-us/products/primary-antibodies/npr-a-npr-b-antibody-epr57192-ab139188'>ab139188</a>) at 1/10000 dilution

Lane 1:

Human heart lysate

Lane 2:

Human kidney lysate

Lane 3:

Mouse brain lysate

Lane 4:

Mouse heart lysate

Lane 5:

Rat brain lysate

Lane 6:

Rat heart lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] - BSA and Azide free (AB248869)
  • WB

Lab

Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] - BSA and Azide free (AB248869)

Blocking buffer and concentration : 5% NFDM/TBST

Loading Control : Anti-GST antibody, ab111947

All lanes:

Western blot - Anti-NPR-A + NPR-B antibody [EPR5719(2)] (<a href='/en-us/products/primary-antibodies/npr-a-npr-b-antibody-epr57192-ab139188'>ab139188</a>) at 1/1000 dilution

Lane 1:

GST-tagged recombinant human Natriuretic Peptide Receptor A protein (aa 495 to 1061) at 0.01 µg

Lane 2:

GST-tagged recombinant human Natriuretic Peptide Receptor B protein (aa 479 to 1047) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 88 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5719(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen for this product shares 95% homology with NPR-A.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab248869 is the carrier-free version of ab139188.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NPR-A and NPR-B are types of natriuretic peptide receptors which operate as transmembrane receptors composed of an extracellular ligand-binding domain a single transmembrane-spanning domain and an intracellular guanylyl cyclase catalytic domain. NPR-A is widely recognized as GC-A while NPR-B is also known as GC-B. These receptors are approximately 120 kDa in size. Both NPR-A and NPR-B are expressed in various tissues including the heart kidneys and vascular smooth muscle cells which suggests a broad physiological role across different body systems.
Biological function summary

These receptors function mainly by binding natriuretic peptides like atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) for NPR-A while C-type natriuretic peptide (CNP) serves as the primary ligand for NPR-B. Their activation leads to the conversion of GTP to cGMP which then functions as a second messenger involved in vasodilation and diuresis. The receptors are essential components of blood pressure regulation and fluid balance through their role in the cGMP complex.

Pathways

For both NPR-A and NPR-B they significantly contribute to the maintenance of cardiovascular homeostasis and kidney function through the natriuretic peptide signaling pathway. They are tightly linked with proteins such as guanylyl cyclase-related proteins and phosphodiesterases which help in the regulation and degradation of cGMP respectively. Furthermore these receptors interact strongly with cyclic nucleotide signal transduction pathways that involve other key proteins including protein kinase G.

NPR-A and NPR-B hold importance due to their role in cardiovascular diseases including hypertension and heart failure. The dysregulation of these receptors alongside ANP and BNP has been associated with these conditions. Moreover they are implicated in skeletal dysplasia disorders due to the involvement of NPR-B in cartilage development where the signaling through NPR-B in chondrocytes plays a significant role. Malfunctions in this receptor system can lead to improper regulation of growth and development linking it with disorders such as achondroplasia alongside CNP.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
See full target information NPR1

Additional targets

NPR2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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