Rabbit Polyclonal NPR-C antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NPR3 aa 250 to C-terminus.
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
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Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
ANPRC, C5orf23, NPRC, NPR3, Atrial natriuretic peptide receptor 3, Atrial natriuretic peptide clearance receptor, Atrial natriuretic peptide receptor type C, ANP-C, ANPR-C, NPR-C
Rabbit Polyclonal NPR-C antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NPR3 aa 250 to C-terminus.
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
The NPR-C also known as natriuretic peptide receptor C functions as an important regulator in the homeostasis of cardiovascular and renal functions. It is also referred to as atrial natriuretic peptide receptor C (ANPRC). This protein does not possess guanylyl cyclase activity unlike its counterparts NPR-A and NPR-B but instead acts via clearance binding of natriuretic peptides preventing them from binding to other receptors. The receptor has a molecular mass of approximately 60 kDa and is widely expressed in tissues such as vascular endothelium kidney and adipose tissue.
NPR-C binds multiple natriuretic peptides including ANP BNP and CNP and modulates their effects by facilitating their clearance from circulation. This receptor does not function as part of a larger protein complex unlike other receptor subtypes. It controls blood volume and pressure by influencing the natriuretic diuretic and vasorelaxant actions of its peptide ligands. NPR-C helps maintain fluid balances and prevents overactivation of hormonal pathways linked to cardiovascular conditions.
NPR-C serves an important role in the natriuretic peptide signaling pathway primarily through the regulation of peptide availability. It physically interacts with several circulating natriuretic peptides but not through second messenger cascades. NPR-C is integral to maintaining the balance between the ANP receptor pathway and the renin-angiotensin-aldosterone system therefore indirectly modulating the functions of NPR-A and NPR-B which are responsible for cyclic GMP-dependent signaling.
NPR-C contributes to the pathophysiology of hypertension and heart failure by overseeing the peptide-hormone balance in the cardiovascular system. Anomalies in NPR-C expression or function can lead to improper regulation of blood pressure and fluid retention. Its interaction with proteins such as angiotensin II receptors can exacerbate the effects of hypertension. Understanding the role of NPR-C in these conditions presents potential therapeutic targets for modulating cardiovascular diseases.
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7.5% SDS PAGE
All lanes: Western blot - Anti-NPR-C antibody (ab97389) at 1/10000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 60 kDa
ab97389, at a 1/200 dilution, staining NPR-C in paraformaldehyde fixed HeLa cell by Immunofluorescence.
The lower image (Panel 2) was merged with DNA probe.
ab97389, at a 1/500 dilution, staining NPR-C in paraffin embedded U87 xenograft by Immunohistochemistry.
Image collected and cropped by CiteAb under a CC-BY license from the publication
NPR-C western blot using anti-NPR-C antibody ab97389. Publication image and figure legend from Peake, N., Su, N., et al., 2013, Arthritis Res Ther, PubMed 23883591.
ab97389 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab97389 please see the product overview.
Comparison of natriuretic peptide receptor 2 (Npr2) and Npr3 expression in non-diseased (grade 0/I) and diseased (grade III to IV) cartilage. Tissues were taken from donors aged 55 to 60 and 80 to 85 years. Paraffin-embedded sections from a single donor aged 60 years were stained with Npr2 or Npr3 antibodies (green) and examined by immunofluoresence microscopy (A). Nuclei (blue) were stained with 4',6-diamidino-2-phenylindole (DAPI). Scale bar represents 10 μM. Negative controls showed no staining (not shown). Inset shows Npr2 (110 kDa) and Npr3 expression (60 kDa) by western blot analysis from the same donor. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
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