AB250024

Anti-NPR-C antibody [EPR12716] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal NPR-C antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

ANPRC, C5orf23, NPRC, NPR3, Atrial natriuretic peptide receptor 3, Atrial natriuretic peptide clearance receptor, Atrial natriuretic peptide receptor type C, ANP-C, ANPR-C, NPR-C

6 Images

Flow Cytometry (Intracellular) - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

This data was developed using ab177954, the same antibody clone in a different buffer formulation.

Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab177954 at a dilution of 1 in 270 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

This data was developed using ab177954, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling NPR-C with unpurified ab177954 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

Lab

Western blot - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

This data was developed using ab177954, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (<a href='/en-us/products/primary-antibodies/npr-c-antibody-epr12716-c-terminal-ab177954'>ab177954</a>) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 60 kDa

Observed band size: 70 kDa

false

Supplier Data

Western blot - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

This data was developed using ab177954, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (<a href='/en-us/products/primary-antibodies/npr-c-antibody-epr12716-c-terminal-ab177954'>ab177954</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human placenta lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 60 kDa

false

Lab

Western blot - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

This data was developed using ab177954, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (<a href='/en-us/products/primary-antibodies/npr-c-antibody-epr12716-c-terminal-ab177954'>ab177954</a>) at 1/1000 dilution

All lanes:

Human tissue lysate at 1/10 dilution

Secondary

All lanes:

HRP anti-rabbit, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 60 kDa

Observed band size: 70 kDa

false

Lab

Western blot - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

This data was developed using ab177954, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (<a href='/en-us/products/primary-antibodies/npr-c-antibody-epr12716-c-terminal-ab177954'>ab177954</a>) at 1/7000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 60 kDa

Observed band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12716

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250024 is the carrier-free version of ab177954.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NPR-C also known as natriuretic peptide receptor C functions as an important regulator in the homeostasis of cardiovascular and renal functions. It is also referred to as atrial natriuretic peptide receptor C (ANPRC). This protein does not possess guanylyl cyclase activity unlike its counterparts NPR-A and NPR-B but instead acts via clearance binding of natriuretic peptides preventing them from binding to other receptors. The receptor has a molecular mass of approximately 60 kDa and is widely expressed in tissues such as vascular endothelium kidney and adipose tissue.

Biological function summary

NPR-C binds multiple natriuretic peptides including ANP BNP and CNP and modulates their effects by facilitating their clearance from circulation. This receptor does not function as part of a larger protein complex unlike other receptor subtypes. It controls blood volume and pressure by influencing the natriuretic diuretic and vasorelaxant actions of its peptide ligands. NPR-C helps maintain fluid balances and prevents overactivation of hormonal pathways linked to cardiovascular conditions.

Pathways

NPR-C serves an important role in the natriuretic peptide signaling pathway primarily through the regulation of peptide availability. It physically interacts with several circulating natriuretic peptides but not through second messenger cascades. NPR-C is integral to maintaining the balance between the ANP receptor pathway and the renin-angiotensin-aldosterone system therefore indirectly modulating the functions of NPR-A and NPR-B which are responsible for cyclic GMP-dependent signaling.

NPR-C contributes to the pathophysiology of hypertension and heart failure by overseeing the peptide-hormone balance in the cardiovascular system. Anomalies in NPR-C expression or function can lead to improper regulation of blood pressure and fluid retention. Its interaction with proteins such as angiotensin II receptors can exacerbate the effects of hypertension. Understanding the role of NPR-C in these conditions presents potential therapeutic targets for modulating cardiovascular diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.

See full target information NPR3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:65 PubMed39972431

2025

Mechanism of histone demethylase KDM5A in osteoporotic fracture healing through epigenetic regulation of the miR-495/SKP2/Runx2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoran Li,Junyan Zhang,Tingting Xu,Zhiying Hao,Yadong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NPR-C antibody [EPR12716] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

Flow Cytometry (Intracellular) - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

Western blot - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

Western blot - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

Western blot - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

Western blot - Anti-NPR-C antibody [EPR12716] - BSA and Azide free (AB250024)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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