Mouse Polyclonal NPRL2 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NPRL2.
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- Medical science monitor : international medical jo 24:1424-14332018Overexpression of Nitrogen Permease Regulator Like-2 (NPRL2) Enhances Sensitivity to Irinotecan (CPT-11) in Colon Cancer Cells by Activating the DNA Damage Checkpoint Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShasha Liu et. al.PubMed 29519997
- Cancer biology & therapy 15:489-952014Relationship between tumor and peripheral blood NPRL2 mRNA levels in patients with colorectal adenoma and colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAi-yun Liu et. al.PubMed 24521741
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human NPRL2. Database link Q8WTW4
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalytic component of the GATOR1 complex, a multiprotein complex that functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway (PubMed:23723238, PubMed:29590090, PubMed:35338845, PubMed:38006878). In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling (PubMed:23723238, PubMed:29590090, PubMed:35338845). In the presence of abundant amino acids, the GATOR1 complex is ubiquitinated and inhibited by GATOR2 (PubMed:23723238, PubMed:36528027). Within the GATOR1 complex, NPRL2 constitutes the catalytic subunit that mediates the GTPase activator activity and under methionine-sufficient conditions, the GTPase activator activity is inhibited by PRMT1 through methylation and consequently inducing timely mTORC1 activation (PubMed:27173016, PubMed:30651352, PubMed:35338845). Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling (PubMed:18616680). Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at 'Tyr-9', 'Tyr-373' and 'Tyr-376' residues (PubMed:18616680). May act as a tumor suppressor (PubMed:18616680). Suppresses cell growth and enhances sensitivity to various anticancer drugs (PubMed:18616680).
Alternative names
TUSC4, NPRL2, GATOR1 complex protein NPRL2, Gene 21 protein, Nitrogen permease regulator 2-like protein, Tumor suppressor candidate 4, G21 protein, NPR2-like protein
Recommended products
Mouse Polyclonal NPRL2 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NPRL2.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human NPRL2. Database link Q8WTW4
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NPRL2 also known as Nitrogen Permease Regulator-Like 2 plays a role in regulating the mTORC1 pathway. It is known to form part of the GATOR1 complex acting as a negative regulator of the mTORC1 signaling. NPRL2 has a molecular mass of approximately 41 kDa. This protein shows expression in various tissues with notable expression in the brain and lung.
- Biological function summary
NPRL2 and its role in the GATOR1 complex importantly manages cellular response to nutrient availability and growth signals. The GATOR1 complex comprises NPRL2 NPRL3 and DEPDC5 which collectively suppress mTORC1 activity under nutrient-poor conditions. This suppression promotes autophagy and inhibits protein synthesis affecting how cells adapt to nutritional stress.
- Pathways
NPRL2 is integral to the amino acid sensing branch of the mTORC1 signaling pathway. It plays a part in the nutrient sensing pathway alongside the proteins NPRL3 and DEPDC5. The mTORC1 pathway essential for controlling cell growth and metabolism depends on the regulated activity of NPRL2 and its partners to respond accurately to metabolic cues.
- Associated diseases and disorders
Altered NPRL2 function links to cancer and epilepsy. Mutations in the GATOR1 complex affecting proteins like DEPDC5 and NPRL3 in conjunction with NPRL2 have associations with tumor growth due to disrupted mTORC1 regulation. In epilepsy especially the focal cortical dysplasia type NPRL2 mutations lead to imbalanced mTORC1 activity contributing to the development of seizures.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-NPRL2 antibody (ab88691)
All lanes: Western blot - Anti-NPRL2 antibody (ab88691) at 1 µg/mL
All lanes: HeLa cell lysate at 50 µg
Predicted band size: 44 kDa
Observed band size: 44 kDa
Western blot - Anti-NPRL2 antibody (ab88691)
All lanes: Western blot - Anti-NPRL2 antibody (ab88691) at 1 µg/mL
Lane 1: NPRL2 transfected 293T cell lysate at 25 µg
Lane 2: non transfected 293T cell lysate at 25 µg
Predicted band size: 44 kDa
Observed band size: ~37 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NPRL2 antibody (ab88691)
ab88691, at 10 μg/ml, staining NPRL2 in HeLa cell by Immunofluorescence.
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