Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)

Rabbit Recombinant Monoclonal NPTX2 antibody. Carrier free. Suitable for IHC-Fr, ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (AB277533), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-Fr	ICC/IF	IP	WB	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Expected	Tested
Mouse	Not recommended	Tested	Tested	Tested	Tested	Expected
Rat	Not recommended	Expected	Expected	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information NPTX2

Function

Likely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner (By similarity).

Alternative names

Neuronal pentraxin-2, NP2, Neuronal pentraxin II, NP-II, NPTX2

Recommended products

Rabbit Recombinant Monoclonal NPTX2 antibody. Carrier free. Suitable for IHC-Fr, ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR24020-38
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab277533 is the carrier-free version of Anti-NPTX2 antibody [EPR24020-38] ab277523.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NPTX2 protein also known as neuronal pentraxin II or NARP weighs approximately 47 kDa. NPTX2 is expressed mostly in the brain especially in neurons. This protein belongs to the neuronal pentraxin family and plays a mechanical role in processes like synaptic plasticity. NPTX2 operates by binding to AMPA receptors influencing their surface expression.

Biological function summary

NPTX2 affects synaptic stabilization by participating in the formation and maintenance of synaptic connections. It forms complexes with AMPA receptor subunits promoting synapses that are more active and facilitating synaptic communication. NPTX2's involvement is essential for normal synaptic function reflecting its significance in neural signaling.

Pathways

More than three words that are not the target name are relevant. NPTX2 plays a role in the glutamatergic signaling pathway an important pathway for synaptic transmission involving excitatory neurotransmitters. NPTX2 works alongside other proteins like GluR1 subunit in this pathway mediating synaptic responses. Through its actions in these pathways NPTX2 contributes to long-term potentiation a process related to learning and memory.

Associated diseases and disorders

NPTX2 has links to neurological conditions such as Alzheimer’s disease and schizophrenia. In Alzheimer's NPTX2 dysregulation associates with synaptic dysfunction possibly involving interactions with beta-amyloid. In schizophrenia altered NPTX2 expression might contribute to glutamatergic signaling imbalances affecting cognitive function. These associations highlight NPTX2's importance in maintaining normal neural processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling NPTX2 with Anti-NPTX2 antibody [EPR24020-38] ab277523 at 1/2000 (0.229 ug/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on mouse cerebrum (PMID:31772567). The section was incubated with Anti-NPTX2 antibody [EPR24020-38] ab277523 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunoprecipitation - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
NPTX2 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with Anti-NPTX2 antibody [EPR24020-38] ab277523 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-NPTX2 antibody [EPR24020-38] ab277523 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: Anti-NPTX2 antibody [EPR24020-38] ab277523 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NPTX2 antibody [EPR24020-38] ab277523 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-NPTX2 antibody [EPR24020-38] (Anti-NPTX2 antibody [EPR24020-38] ab277523)
Predicted band size: 47 kDa
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling NPTX2 with Anti-NPTX2 antibody [EPR24020-38] ab277523 at 1/2000 (0.229 ug/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Positive staining on human cerebrum. The section was incubated with Anti-NPTX2 antibody [EPR24020-38] ab277523 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 100% Methanol permeabilized Beta-TC-6 cells labelling NPTX2 with Anti-NPTX2 antibody [EPR24020-38] ab277523 at 1/50 (9.16 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Beta-TC-6 cells. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Western blot - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
NPTX2 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 8786423, 28440221).
Exposure time: 15 seconds.
All lanes: Western blot - Anti-NPTX2 antibody [EPR24020-38] (Anti-NPTX2 antibody [EPR24020-38] ab277523) at 1/1000 dilution
Lane 1: Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse testis tissue lysate at 20 µg
Lane 4: Mouse kidney tissue lysate at 20 µg
Lane 5: Mouse lung tissue lysate at 20 µg
Lane 6: Rat brain tissue lysate at 20 µg
Lane 7: Rat testis tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 50-60 kDa
Flow Cytometry (Intracellular) - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Beta-TC-6 (Mouse pancreas insulinoma beta cell) cells labelling NPTX2 with Anti-NPTX2 antibody [EPR24020-38] ab277523 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor^®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
NPTX2 was immunoprecipitated from 0.35 mg Human brain tissue lysate with Anti-NPTX2 antibody [EPR24020-38] ab277523 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-NPTX2 antibody [EPR24020-38] ab277523 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human brain tissue lysate 10 ug
Lane 2: Anti-NPTX2 antibody [EPR24020-38] ab277523 IP in Human brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NPTX2 antibody [EPR24020-38] ab277523 in human brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-NPTX2 antibody [EPR24020-38] (Anti-NPTX2 antibody [EPR24020-38] ab277523)
Predicted band size: 47 kDa
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling NPTX2 with Anti-NPTX2 antibody [EPR24020-38] ab277523 at 1/2000 (0.229 ug/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on islet of human pancreas. The section was incubated with Anti-NPTX2 antibody [EPR24020-38] ab277523 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
NPTX2 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 8786423, 28440221).
Negative: human kidney, human lung (PMID: 8530029, 8786423).
Exposure times: Lane 1: 15 secondsLane 2-5: 3 minutes.
All lanes: Western blot - Anti-NPTX2 antibody [EPR24020-38] (Anti-NPTX2 antibody [EPR24020-38] ab277523) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg
Lane 2: Human pancreas tissue lysate at 20 µg
Lane 3: Human testis tissue lysate at 20 µg
Lane 4: Human kidney tissue lysate at 20 µg
Lane 5: Human lung tissue lysate at 20 µg
Secondary
All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 50-60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling NPTX2 with Anti-NPTX2 antibody [EPR24020-38] ab277523 at 1/2000 (0.229 ug/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with Anti-NPTX2 antibody [EPR24020-38] ab277523 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
NPTX2 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 8786423, 28440221).
Exposure time: 15 seconds.
All lanes: Western blot - Anti-NPTX2 antibody [EPR24020-38] (Anti-NPTX2 antibody [EPR24020-38] ab277523) at 1/1000 dilution
All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 50-60 kDa
Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
IHC image of NPTX2 staining in a section of frozen normal human pancreas performed on a Leica Biosystems BOND® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with Anti-NPTX2 antibody [EPR24020-38] ab277523, 0.5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
IHC image of NPTX2 staining in a section of frozen normal human cerebral cortex performed on a Leica Biosystems BOND® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with Anti-NPTX2 antibody [EPR24020-38] ab277523, 0.5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR24020-38] - BSA and Azide free (ab277533)
Negative tissue image: IHC image of NPTX2 staining in a section of frozen normal human heart performed on a Leica Biosystems BOND® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with Anti-NPTX2 antibody [EPR24020-38] ab277523, 0.5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
This data was developed using Anti-NPTX2 antibody [EPR24020-38] ab277523, the same antibody clone in a different buffer formulation.