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AB305257

Anti-NPTX2 antibody [EPR27176-81]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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Rabbit Recombinant Monoclonal NPTX2 antibody. Suitable for Dot, IHC-Fr, ICC/IF and reacts with Human, Mouse, Rat samples.

View Alternative Names

Neuronal pentraxin-2, NP2, Neuronal pentraxin II, NP-II, NPTX2

8 Images
Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling NPTX2 with ab305257 at 1/50 (9.3 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Negative control : confocal image showing no staining on mouse kidney (PMID : 8530029). The nuclear counterstain was DAPI (Blue). The section was incubated with ab305257 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (fresh) tissue labeling NPTX2 with ab305257 at 1/50 (9.3 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Negative control : confocal image showing no staining on mouse lung (PMID : 8530029). The nuclear counterstain was DAPI (Blue). The section was incubated with ab305257 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling NPTX2 with ab305257 at 1/50 (9.3 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Negative control : confocal image showing no staining on rat kidney (PMID : 8530029). The nuclear counterstain was DAPI (Blue). The section was incubated with ab305257 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung (fresh) tissue labeling NPTX2 with ab305257 at 1/50 (9.3 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Negative control : confocal image showing no staining on rat lung (PMID : 8530029). The nuclear counterstain was DAPI (Blue). The section was incubated with ab305257 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh) tissue labeling NPTX2 with ab305257 at 1/50 (9.3 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Confocal image showing positive staining on mouse hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab305257 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTX2 antibody [EPR27176-81] (AB305257)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh) tissue labeling NPTX2 with ab305257 at 1/50 (9.3 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Confocal image showing positive staining on rat hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab305257 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-NPTX2 antibody [EPR27176-81] (AB305257)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NPTX2 antibody [EPR27176-81] (AB305257)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cells labelling NPTX2 with ab305257 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Beta-TC-6 cell line. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Dot Blot - Anti-NPTX2 antibody [EPR27176-81] (AB305257)
  • Dot

Supplier Data

Dot Blot - Anti-NPTX2 antibody [EPR27176-81] (AB305257)

Dot blot analysis of NPTX2 using ab305257 at 1 : 1000 (0.466 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Lane 1 : Mouse NPTX2 recombinant protein fragment Lane 2 : Human NPTX2 recombinant protein fragment Lane 3 : Human NPTX1 recombinant protein fragment Lane 4 : Human NPTXR recombinant protein fragment Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST

  • Carrier free

    Anti-NPTX2 antibody [EPR27176-81] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27176-81

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, Dot, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NPTX2 protein also known as neuronal pentraxin II or NARP weighs approximately 47 kDa. NPTX2 is expressed mostly in the brain especially in neurons. This protein belongs to the neuronal pentraxin family and plays a mechanical role in processes like synaptic plasticity. NPTX2 operates by binding to AMPA receptors influencing their surface expression.
Biological function summary

NPTX2 affects synaptic stabilization by participating in the formation and maintenance of synaptic connections. It forms complexes with AMPA receptor subunits promoting synapses that are more active and facilitating synaptic communication. NPTX2's involvement is essential for normal synaptic function reflecting its significance in neural signaling.

Pathways

NPTX2 plays a role in the glutamatergic signaling pathway an important pathway for synaptic transmission involving excitatory neurotransmitters. NPTX2 works alongside other proteins like GluR1 subunit in this pathway mediating synaptic responses. Through its actions in these pathways NPTX2 contributes to long-term potentiation a process related to learning and memory.

NPTX2 has links to neurological conditions such as Alzheimer’s disease and schizophrenia. In Alzheimer's NPTX2 dysregulation associates with synaptic dysfunction possibly involving interactions with beta-amyloid. In schizophrenia altered NPTX2 expression might contribute to glutamatergic signaling imbalances affecting cognitive function. These associations highlight NPTX2's importance in maintaining normal neural processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Likely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner (By similarity).
See full target information NPTX2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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