Anti-NPTXR antibody [EPR25684-3] - BSA and Azide free

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTXR antibody [EPR25684-3] - BSA and Azide free (AB289869)

This data was developed using ab289860, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labelling NPTXR with ab289860 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on mouse cerebrum. The nuclear counterstain is DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR antibody [EPR25684-3] - BSA and Azide free (AB289869)

This data was developed using ab289860, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling NPTXR with ab289860 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control : no staining on mouse liver. The section was incubated with ab289860 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is preabsorbed LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTXR antibody [EPR25684-3] - BSA and Azide free (AB289869)

This data was developed using ab289860, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labelling NPTXR with ab289860 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution (Green). No staining on mouse skeletal muscle. The nuclear counterstain is DAPI (Blue).

Negative control : (PMID : 9261167).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR antibody [EPR25684-3] - BSA and Azide free (AB289869)

This data was developed using ab289860, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling NPTXR with ab289860 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab289860 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is preabsorbed LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR antibody [EPR25684-3] - BSA and Azide free (AB289869)

This data was developed using ab289860, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling NPTXR with ab289860 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab289860 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is preabsorbed LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR antibody [EPR25684-3] - BSA and Azide free (AB289869)

This data was developed using ab289860, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling NPTXR with ab289860 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control : no staining on rat liver (PMID : 9261167). The section was incubated with ab289860 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is preabsorbed LeicaDS9800 (Bond™ Polymer Refine Detection).

• WB

Supplier Data

Western blot - Anti-NPTXR antibody [EPR25684-3] - BSA and Azide free (AB289869)

This data was developed using ab289860, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as a blocking and diluting buffer.

This antibody has no cross-reaction with human NPTX1 and NPTX2.

All lanes:

Western blot - Anti-NPTXR antibody [EPR25684-3] (<a href='/en-us/products/primary-antibodies/nptxr-antibody-epr25684-3-ab289860'>ab289860</a>) at 1/1000 dilution

Lane 1:

His-tagged human NPTXR recombinant protein 10ng

Lane 2:

His-tagged human NPTX1 recombinant protein 10ng

Lane 3:

Human NPTX2 recombinant protein 10ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 53 kDa

Observed band size: 30 kDa

false

Exposure time: 180s