Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (AB289861)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NPTXR+NPTX2+NPTX1 with ab289861 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab289861 for 30 mins at room temperature.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (AB289861)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling NPTXR+NPTX2+NPTX1 with ab289861 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control : no staining on human liver. The section was incubated with ab289861 for 30 mins at room temperature.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (AB289861)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labelling NPTXR+NPTX2+NPTX1 with ab289861 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution (Green). Negative control (PMID : 9261167). No staining on rat skeletal muscle (fresh). The nuclear counterstain is DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (AB289861)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labelling NPTXR+NPTX2+NPTX1 with ab289861 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on rat cerebrum. The nuclear counterstain is DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (AB289861)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling NPTXR+NPTX2+NPTX1 with ab289861 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab289861 for 30 mins at room temperature.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (AB289861)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labelling NPTXR+NPTX2+NPTX1 with ab289861 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on mouse cerebrum (fresh). The nuclear counterstain is DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (AB289861)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling NPTXR+NPTX2+NPTX1 with ab289861 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab289861 for 30 mins at room temperature.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (AB289861)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labelling NPTXR+NPTX2+NPTX1 with ab289861 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution (Green). Negative control (PMID : 9261167). No staining on mouse skeletal muscle (fresh). The nuclear counterstain is DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) secondary antibody at 1/1000 dilution.

• WB

Supplier Data

Western blot - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (AB289861)

Blocking and diluting buffer and concentration : 5% NFDM/TBS.

Eposure times :

Lane1-2 : 180 seconds;
Lane3-8 : 70 seconds.

Samples are non-boiled as boiling may cause protein aggregates.
The observed MW are consistent with what has been described in the literature (PMID : 32847597, PMID : 32273328, PMID : 28440221)
Low expression : Hela, skeletal muscle, spleen (PMID : 32847597, PMID : 32273328, PMID : 28440221)
The bands nearby 40 kDa could be non-specific bands.

All lanes:

Western blot - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (ab289861) at 1/1000 dilution

Lane 1:

IMR-32 (Human neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 40 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Lane 6:

Rat brain tissue lysate at 20 µg

Lane 7:

Rat skeletal muscle tissue lysate at 40 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 53 kDa

false

• WB

Supplier Data

Western blot - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (AB289861)

Blocking and diluting buffer and concentration : 5% NFDM/TBS.

Eposure times : 5.5 seconds

All lanes:

Western blot - Anti-NPTXR + NPTX2 + NPTX1 antibody [EPR25684-9] (ab289861) at 1/1000 dilution

Lane 1:

His-tagged human NPTXR recombinant protein fragment 10ng

Lane 2:

His-tagged human NPTX1 full length recombinant protein (without signal peptide) 10ng

Lane 3:

Human NPTX2 full length recombinant protein (without signal peptide) 10ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false